EGCG和茶氨酸对细胞氧化损伤的协同保护和修复作用研究

doi:10.13305/j.cnki.jts.2014.03.005

茶叶科学 ›› 2014, Vol. 34 ›› Issue (3): 239-247.doi: 10.13305/j.cnki.jts.2014.03.005

EGCG和茶氨酸对细胞氧化损伤的协同保护和修复作用研究

刘晓慧¹, 揭国良¹, 林康², 吴媛媛¹, 王岳飞¹, 屠幼英^1,*

1. 浙江大学农业与生物技术学院茶学系,浙江杭州 310058;
2. 景宁畲族自治县,浙江丽水 323500

收稿日期:2013-08-16 修回日期:2013-12-10 出版日期:2014-06-15 发布日期:2019-09-03
通讯作者: *youytu@zju.edu.cn
作者简介:刘晓慧（1985— ）,女,山东鄄城人,博士研究生,主要从事茶叶深加工及保健功能方面的研究。
基金资助:
景宁茶资源生化特征与惠明白茶提高免疫力活性功能研究（H20130018）、浙江大学与Philips茶叶合作项目（I2010007Q）

Research on Synergistic Protection and Repairing Effects of EGCG and Theanine in Oxidative Damaged Cells

LIU Xiaohui¹, JIE Guoliang¹, LIN Kang², WU Yuanyuan¹, WANG Yuefei¹, TU Youying^1,*

1. Department of Tea Science, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China;
2. Jingning She Autonomous County, Lishui 323500,China

Received:2013-08-16 Revised:2013-12-10 Online:2014-06-15 Published:2019-09-03

摘要/Abstract

摘要： 研究了表没食子儿茶素没食子酸酯（EGCG）和茶氨酸两种茶叶主要活性成分单独和协同清除2, 2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐（ABTS）、1, 1-二苯基-2-三硝基苯肼（DPPH）和羟自由基的效果,并通过建立细胞氧化损伤模型,测定两种化合物对氧化损伤细胞的氧化水平和抗氧化酶活性的影响。结果表明：EGCG和茶氨酸对ABTS和羟自由基的清除有协同增效作用,对DPPH自由基无协同作用;在细胞水平,EGCG和茶氨酸可协同降低细胞内活性氧自由基及脂质过氧化水平,提高谷胱甘肽过氧化物酶的活性,即两者共同作用可协同降低细胞所受的氧化损伤,提高细胞抗氧化能力。

关键词: EGCG, 茶氨酸, 抗氧化, 协同作用

Abstract: This paper investigated the scavenging capacities of EGCG and theanine, the two main active components in tea, alone or synergistically on ABTS, DPPH and hydroxyl radicals in vitro were investigated. The oxidative injury experimental model of mouse macrophage (RAW 264.7) by H₂O₂ was established for measuring the oxidative level and antioxidative enzyme activities by EGCG and theanine. The results showed that EGCG and theanine showed synergistic effect on the scavenging of ABTS and hydroxyl radical except for the DPPH radical. EGCG and theanine could synergistically reduce the contents of reactive oxygen species and lipid peroxidation, and enhance the activity of GSH-Px on RAW 264.7, which meant that the combination of above two compounds could synergistically reduce the oxidative damage of the cells through improving the antioxidant capacity.

Key words: EGCG, theanine, antioxidant, synergistic effect

中图分类号:

刘晓慧, 揭国良, 林康, 吴媛媛, 王岳飞, 屠幼英. EGCG和茶氨酸对细胞氧化损伤的协同保护和修复作用研究[J]. 茶叶科学, 2014, 34(3): 239-247. doi: 10.13305/j.cnki.jts.2014.03.005.

LIU Xiaohui, JIE Guoliang, LIN Kang, WU Yuanyuan, WANG Yuefei, TU Youying. Research on Synergistic Protection and Repairing Effects of EGCG and Theanine in Oxidative Damaged Cells[J]. Journal of Tea Science, 2014, 34(3): 239-247. doi: 10.13305/j.cnki.jts.2014.03.005.

参考文献 30

[1]	Matsuoka K, lsowa N, Yoshimura T, et a1. Green tea polyphenol blocks H2O2-induced interleukin-8 production from human alveolar epithelial cells[J]. Cytokine, 2002, 18: 266-273.
[2]	Wu Y Y, Li W, Xu Y, et al. Evaluation of the antioxidant effects of four main theaflavin derivatives through chemiluminescence and DNA damage analyses[J]. Journal of Zhejiang University-Science B (Biomed & Biotechnol), 2011, 12(9): 744-751.
[3]	Chung S Yang, Xin Wang, Gang Lu, et al. Cancer prevention by tea: animal studies, molecular mechanisms and human relevance[J]. Cancer, 2009, 9: 429-439.
[4]	刘国辉, 谢鼎华, 朱纲华, 等. EGCG对过氧化氢造成的螺旋神经节细胞损害的抑制作用[J]. 听力学及言语疾病杂志, 2004, 5(12): 317-320.
[5]	杨志辉, 杨华, 刘仲华. EGCG清除自由基和抑制血液脂质过氧化作用的研究[J]. 湖南农业科学, 2009(5): 18-20.
[6]	苏畅, 刘银花, 卢章显. 表没食子儿茶素没食子酸酯对人肝细胞氧化损伤的保护作用[J]. 安徽农业科学, 2009, 37(6): 2561-2563.
[7]	胡秀芳, 柴立红, 陈集双, 等. 氧化应激对Vero细胞的损伤及EGCG的保护作用[J]. 茶叶科学, 2003, 23(2): 105-109.
[8]	Li G L, Kang J J, Yao X X, et al. The component of green tea, L-theanine protects human hepatic L02 cells from hydrogen peroxide-induced apoptosis[J]. European Food Research Technology, 2011, 233: 427-435.
[9]	吴琳琳, 常忠义, 高红亮, 等. 抗氧化剂TBHQ、VE和TP对罗非鱼非漂洗鱼糜的抗氧化作用[J]. 西北农林科技大学学报, 2012, 40(4): 185-190.
[10]	范柳萍, 鲁璐, 刘元法, 等. 高温煎炸棕榈油体系抗氧化剂的选择[J]. 中国油脂, 2012, 37(3): 40-43.
[11]	王莹. 茶多酚的抗氧化和抑菌活性及其增效剂[J]. 生物学杂志, 2007, 24(5): 54-56.
[12]	王红芹. 茶多酚和维生素E对肉仔鸡抗氧化性能的影响[J]. 安徽农业科学, 2012, 40(8): 4588-4594.
[13]	陈玉香, 刘阳, 周道玮. 茶多酚对豆油及猪油的抗氧化作用[J]. 食品科学, 2001, 22(11): 27-29.
[14]	李清钊, 关维俊, 刘英莉, 等. 茶多酚联合维生素C对阿霉素肾毒性的保护作用[J]. 工业卫生与职业病, 2003, 29(5): 273-275.
[15]	Li W, Wu J X, Tu Y Y.Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells[J]. Journal of Zhejiang University-Science B (Biomed & Biotechnol), 2010, 11(6): 458-464.
[16]	Jeong Y M, Choi Y G, Kim D S, et al. Cytoprotective effect of green tea extract and quercetin against hydrogen peroxide-induced oxidative stress[J]. Archives of Pharmacal Research, 2005, 28: 1251-1256.
[17]	Afzal M, Safer A M, Al-Bloushi S.CoQ9 potentiates green tea antioxidant activities in Wistar rats[J]. Bio Factors, 2005, 25: 255-259.
[18]	Ai Yoto, Mao Motoki, Sato Murao, et al. Effects of L-theanine or caffeine intake on changes in blood pressure under physical and psychological stresses[J]. Journal of Physiological Anthropology, 2012, 31: 28.
[19]	Owen G, Nobre A C, Rycroft J, et al. Theanine, a natural constituent in tea, and its effect on mental state[J]. Nutrition and Dietetics, 2006, 63: 44-45.
[20]	Lu K, Gray M A, Oliver C, et al. The acute effects of L-theanine in comparison with alprazolam on anticipatory anxiety in humans[J]. Human Psychopharm, 2004, 19: 457-465.
[21]	Yasuhiro TAKAGI, Shigekazu KURIHARA, Natsumi HIGASHI, et al. Combined Administration of L-Cystine and L-Theanine Enhances Immol/Lune Functions and Protects against Influenza Virus Infection in Aged Mice[J]. Journal of Veterinary Medical Science, 2010, 72(2): 157-165.
[22]	Sun L Y, Tian X, Gou L S, et al. Beneficial synergistic effects of concurrent treatment with theanine and caffeine against cerebral ischemia-reperfusion injury in rats[J]. Canadian Journal of Physiology and Pharmacology, 2013, 91(7): 562-569.
[23]	Zheng G D, Sayama K, Okubo T, et al. Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice[J]. In Vivo, 2004, 18(1): 55-62.
[24]	刘晓慧, 孟庆, 李伟, 等. 茶多酚类化合物与维生素协同抗氧化机理研究进展[J]. 热带作物学报, 2012, 33(12): 2305-2310.
[25]	Dudonne S, Vitrac X, Coutiere P, et al. Comparative Study of Antioxidant Properties and Total Phenolic Content of 30 Plant Extracts of Industrial Interest Using DPPH, ABTS, FRAP, SOD, and ORAC Assays[J]. Journal of Agricultural and Food Chemistry, 2009, 57(5): 1768-1774.
[26]	李贵荣, 杨胜圆. 党参多糖的提取及其对活性氧自由基的清除作用[J]. 化学世界, 2001, 42(8): 21-22.
[27]	Tang X, Yao K, Zhang L, et al. Honokiol inhibits H< sub> 2</sub> O< sub> 2</sub>-induced apoptosis in human lens epithelial cells via inhibition of the mitogen-activated protein kinase and Akt pathways[J]. European Journal of Pharmacology, 2011, 650(1): 72-78.
[28]	Sugiyama T, SadzukaY. Enhancing effects of green tea components on the antitumor activity of adriamyc in against M5076 ovarian sarcoma[J]. Cancer Letters, 1998, 133(1): 19-26.
[29]	Sugiyama T, Sadzuka Y.Combination of theanine with doxorubicin inhibits hepatic metastasis of M5076 ovarian sarcoma[J]. Clinical Cancer Research, 1999, 5(2): 413-416.
[30]	Lu Y N, Zhang J S, Wan X C, et al. Intestinal transport of pure theanine and theanine in green tea extract: Green tea components inhibit theanine absorption and promote theanine excretion[J]. Food Chemistry, 2011(125): 277-281.

EGCG和茶氨酸对细胞氧化损伤的协同保护和修复作用研究

Research on Synergistic Protection and Repairing Effects of EGCG and Theanine in Oxidative Damaged Cells

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 30

相关文章 15

Metrics

本文评价

推荐阅读 10

[1]	陈珂, 王苑竹, 杨晓颖, 张冬英, 朱强强. 壳聚糖复合β-乳球蛋白负载EGCG纳米粒的制备及其对糖尿病小鼠血糖的影响[J]. 茶叶科学, 2022, 42(5): 731-739.
[2]	俞蓉欣, 郑芹芹, 陈红平, 张劲松, 张相春. 儿茶素生物医用纳米材料研究进展[J]. 茶叶科学, 2022, 42(4): 447-462.
[3]	张旖旎, 吉铮. 表没食子儿茶素没食子酸酯（EGCG）研究的文献计量分析[J]. 茶叶科学, 2022, 42(3): 423-434.
[4]	杨高中, 彭群华, 张悦, 施江, 林智, 吕海鹏. 厌氧处理对不同类型茶叶的氨基酸组成及生物活性的影响[J]. 茶叶科学, 2022, 42(2): 222-232.
[5]	马冰凇, 王佳菜, 徐成成, 任小盈, 马存强, 周斌星. 不同仓储期普洱茶（生茶）中酚类成分差异及其对体外抗氧化能力的影响[J]. 茶叶科学, 2022, 42(1): 51-62.
[6]	黄秋萍, 谢晨阳, 李新宇, 金斌, 曾榛, 钱波, 戴玲, 宋家乐. L-茶氨酸改善来曲唑诱导的多囊卵巢综合征大鼠临床症状[J]. 茶叶科学, 2021, 41(6): 831-842.
[7]	焦海珍, 邵陈禹, 陈建姣, 张晨禹, 陈佳豪, 李云飞, 沈程文. 重度遮阴及复光条件下茶树根系的生理响应及抗氧化酶活性动态变化[J]. 茶叶科学, 2021, 41(5): 695-704.
[8]	陈美艳, 刘芬, 林勇, 左高隆, 左颖鹏, 刘仲华. L-茶氨酸对CUMS抑郁大鼠海马和肠道损伤的干预作用研究[J]. 茶叶科学, 2021, 41(4): 511-524.
[9]	毛鹏, 王丽鸳, 白培贤, 韦康, 阮丽, 张亚真, 成浩. 类茶氨酸合成酶基因家族在番茄中的鉴定及表达研究[J]. 茶叶科学, 2021, 41(2): 173-183.
[10]	金可, 黄建安, 熊立瑰, 刘硕谦, 覃小洪, 彭靖, 李银花, 李娟. 黄化变异茶树石门黄叶理化分析及茶氨酸相关基因表达研究[J]. 茶叶科学, 2021, 41(1): 40-47.
[11]	张建勇, 陈琳, 崔宏春, 王伟伟, 薛金金, 熊春华, 江和源. PBD和RSM联用优化聚酯型儿茶素A化学合成技术参数[J]. 茶叶科学, 2020, 40(1): 51-62.
[12]	曾立, 肖文军. L-茶氨酸防治脑疾病研究进展[J]. 茶叶科学, 2019, 39(2): 193-202.
[13]	彭影琦, 肖文军, 张盛, 袁冬寅, 林玲, 周阳, 龚志华. L-茶氨酸对小鼠肠道形态结构及游离氨基酸的影响[J]. 茶叶科学, 2019, 39(1): 43-54.
[14]	曾琪, 任发政, 雷新根, 侯彩云. 白茶体外抗氧化与体内抗衰老作用的研究[J]. 茶叶科学, 2018, 38(6): 615-624.
[15]	魏吉鹏, 李鑫, 王朝阳, 李洋, 张兰, 沈晨, 颜鹏, 张丽平, 韩文炎. 外源水杨酸甲酯对高温胁迫下茶树光合作用和抗氧化酶的影响[J]. 茶叶科学, 2018, 38(4): 353-362.