茶树cpDNA测序及基于cpDNA序列的山茶属植物亲缘关系研究

doi:10.13305/j.cnki.jts.2014.04.008

茶叶科学 ›› 2014, Vol. 34 ›› Issue (4): 371-380.doi: 10.13305/j.cnki.jts.2014.04.008

茶树cpDNA测序及基于cpDNA序列的山茶属植物亲缘关系研究

陈春梅, 马春雷, 马建强, 刘声传, 陈亮^*

中国农业科学院茶叶研究所国家茶树改良中心,农业部茶树生物学与资源利用重点实验室,浙江杭州 310008

收稿日期:2014-05-04 修回日期:2014-05-27 出版日期:2014-08-15 发布日期:2019-09-03
通讯作者: *liangchen@mail.tricaas.com
作者简介:陈春梅（1987— ）,女,福建三明人,硕士研究生,主要从事茶树分子生物学研究。
基金资助:
国家茶叶产业技术体系项目（CARS-23）、国家自然科学基金项目（30901159、31170624、31100504）

Sequencing of Chloroplast Genome of Camellia sinensis and Genetic Relationship for Camellia Plants Based on Chloroplast DNA Sequences

CHEN Chunmei, MA Chunlei, MA Jianqiang, LIU Shengchuan, CHEN Liang^*

Tea Research Institute, Chinese Academy of Agricultural Sciences, National Center for Tea Improvement/ Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China

Received:2014-05-04 Revised:2014-05-27 Online:2014-08-15 Published:2019-09-03

摘要/Abstract

摘要： 叶绿体基因组在物种鉴定、系统进化分析及亲缘关系研究等领域应用前景广阔。本文应用Illumina高通量测序技术对龙井43（Camellia sinensis cv. Longjing 43）的叶绿体全基因组进行测序;利用叶绿体trnL-trnF序列研究茶树及其近缘植物的亲缘关系。结果表明,龙井43叶绿体基因组全长为157 096 bp,反向互补重复区（Inverted repeat, IR）为26 080 bp,小单拷贝区（Small single copy region, SSC）、大单拷贝区（Large single copy region, LSC）分别为18 283 bp、86 653 bp。共注释叶绿体基因133个,其中蛋白编码基因86个,rRNA基因8个,tRNA基因39个。对所选植物的trnL-trnF序列进行比对,序列长度变异范围为481~501 bp,序列最长为岔河大茶,最短为金花茶,基于此序列构建亲缘关系树,山茶属茶组植物聚成一个组。研究结果对茶树优良品种培育及山茶属植物亲缘关系的研究具有重要价值。

关键词: 茶树, 叶绿体基因组, 高通量测序, 亲缘关系

Abstract: Chloroplast genome sequences have comprehensive application prospects in species identification, phylogeny analyses and transgenic breeding. The complete chloroplast genome of Camellia sinensis cv. Longjing 43 was sequenced using Illumina sequencing technology. The study of genetic relationship for tea plant and its closely related species based on chloroplast DNA sequences trnL-trnF. The results showed that the chloroplast genome of ‘Longjing 43’ was found to be 157 096 bp in length which included a pair of inverted repeats (IRs) of 26 080 bp, separated by a small singlecopy region of 18 283 bp and a large single-copy region (LSC) of 86 653 bp. A total of 133 predicted genes including 86 protein-coding genes, 8 ribosomal RNA genes and 39 tRNA genes were identified. Sequence alignment of trnL-trnF for Plants were selected, the sequence of trnL-trnF ranged from 481 bp to 501 bp in length, the longest length of trnL-trnF region was 501 bp in C. tachangensis, the shortest was 481 bp in C. nitidissima. Phylogenetic analysis showed that all of the Sect. Thea being organized in the same clade. The results will play an important role for tea breeding and phylogenetic relationship study of tea plant and other Camellia species.

Key words: tea plant, chloroplast genome, high-throughput sequencing, genetic relationship

中图分类号:

S571.1
S326

陈春梅, 马春雷, 马建强, 刘声传, 陈亮. 茶树cpDNA测序及基于cpDNA序列的山茶属植物亲缘关系研究[J]. 茶叶科学, 2014, 34(4): 371-380. doi: 10.13305/j.cnki.jts.2014.04.008.

CHEN Chunmei, MA Chunlei, MA Jianqiang, LIU Shengchuan, CHEN Liang. Sequencing of Chloroplast Genome of Camellia sinensis and Genetic Relationship for Camellia Plants Based on Chloroplast DNA Sequences[J]. Journal of Tea Science, 2014, 34(4): 371-380. doi: 10.13305/j.cnki.jts.2014.04.008.

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茶树cpDNA测序及基于cpDNA序列的山茶属植物亲缘关系研究

Sequencing of Chloroplast Genome of Camellia sinensis and Genetic Relationship for Camellia Plants Based on Chloroplast DNA Sequences

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