茶叶黄酮类物质的双水相系统纯化及抗氧化研究

doi:10.13305/j.cnki.jts.2012.04.003

茶叶科学 ›› 2012, Vol. 32 ›› Issue (4): 289-296.doi: 10.13305/j.cnki.jts.2012.04.003

茶叶黄酮类物质的双水相系统纯化及抗氧化研究

吴祥庭¹, 王爱银², 周化斌¹, 李国兴², 徐姗姗¹

1. 浙江温州大学生命与环境科学学院,浙江温州 325000;
2. 浙江温州大学化学与材料工程学院,浙江温州 325000

收稿日期:2011-12-30 修回日期:2012-03-20 出版日期:2012-08-15 发布日期:2019-09-05
作者简介:吴祥庭（1965— ）,男,浙江东阳人,副教授,主要从事食品生物工程方向的研究。E-mail: wuxt222@163.com
基金资助:
浙江省科技厅项目（No. 2010C32005）

Study on Extraction by Aqueous Two-phase System and Anti-oxidation of Flavonoids from Tea

WU Xiang-ting¹, WANG Ai-yin², ZHOU Hua-bin¹, LI Guo-xing², XU Shan-shan¹

1. School of Life and Environmental Science, Wenzhou University, Wenzhou 325000, China;
2. College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325000, China

Received:2011-12-30 Revised:2012-03-20 Online:2012-08-15 Published:2019-09-05

摘要/Abstract

摘要： 采用PEG/盐的双水相系统纯化茶叶黄酮,探究萃取机理及其抗氧化活性。以茶叶为原料,茶叶黄酮得率为指标;采用单因素和响应面法试验设计,研究PEG的种类和用量、盐的种类和用量、温度、pH对茶叶黄酮提取率的影响,根据Box-Behnken中心组合方法进行3因素（Na₂SO₄的质量分数、PEG600、MgCl₂添加量）3水平的试验设计,进行响应面（RSM）分析。通过分析还原力和DPPH体系,评估不同浓度黄酮的抗氧化活性,并与参照抗氧化剂BHT和Vc进行比较。结果显示最佳条件：pH为5,萃取温度30℃,5mL 15% Na₂SO₄,6g PEG600,MgCl₂的添加量为2.9%,萃取率为96.31%。本方法所形成双水相体系,操作简便,萃取率高,方法重现性好;茶叶黄酮有较强的铁还原能力和清除DPPH自由基能力,茶叶黄酮是一个潜在的天然安全的有效的抗氧化剂。

关键词: 茶叶, 黄酮, 双水相体系, 响应面法, 抗氧化

Abstract: To explore antioxidation capability and the extraction mechanism of the separation-purification of flavonoids from tea in aqueous two-phase systems, flavonoids from tea was taken as an index, to optimize the conditions for the extraction of flavonoids from tea, the effect of each factor such as weight and amounts of PEG, kinds and amounts of salt, temperature, pH have been studied by single factor and response surface methodology. Based on the single factor experiments, a three-factor (the amount of Na₂SO₄, PEG600, MgCl₂) and three-level experiment design has been developed by Box-Behnken central composite design method. The antioxidant activity of different concentration flavonoids were assessed through reducing power assay, Scavenging effects on DPPH radicals and results were compared to reference antioxidant BHT and Vc. The result showed that the optimum conditions as follows, pH 5, the extracting temperature 30℃, the amount of 5mL Na₂SO₄ 15%, the amount of 6g PEG600, the amount of MgCl₂ 2.9%, the extraction field 96.31%. The two aqueous phase system for flavonoid extraction is easy to get good reproductivity, and applied in mass production. The flavonoids from tea showed strong antioxidant activity. The flavonoids from tea are a potential product of natural safe and effective antioxidant.

Key words: tea, flavonoid, aqueous two-phase system, response surface analysis, anti-oxidation

中图分类号:

TS272
Q946.8

吴祥庭, 王爱银, 周化斌, 李国兴, 徐姗姗. 茶叶黄酮类物质的双水相系统纯化及抗氧化研究[J]. 茶叶科学, 2012, 32(4): 289-296. doi: 10.13305/j.cnki.jts.2012.04.003.

WU Xiang-ting, WANG Ai-yin, ZHOU Hua-bin, LI Guo-xing, XU Shan-shan. Study on Extraction by Aqueous Two-phase System and Anti-oxidation of Flavonoids from Tea[J]. Journal of Tea Science, 2012, 32(4): 289-296. doi: 10.13305/j.cnki.jts.2012.04.003.

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茶叶黄酮类物质的双水相系统纯化及抗氧化研究

Study on Extraction by Aqueous Two-phase System and Anti-oxidation of Flavonoids from Tea

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