茯砖茶对H2O2诱发LLC-PK1细胞损伤的保护作用

doi:10.13305/j.cnki.jts.2012.06.004

茶叶科学 ›› 2012, Vol. 32 ›› Issue (6): 539-542.doi: 10.13305/j.cnki.jts.2012.06.004

茯砖茶对H₂O₂诱发LLC-PK1细胞损伤的保护作用

宋家乐

釜山国立大学食品科学和营养学科,韩国釜山 609-735

收稿日期:2012-06-04 修回日期:2012-08-06 出版日期:2012-12-15 发布日期:2019-09-05
作者简介:宋家乐（1983— ）,男,江苏扬州人,博士,主要从事分子营养学、植物化学研究。E-mail: bio.paul@hotmail.com

Protective Effect of Fuzhuan Brick Tea on H₂O₂-Induced Cellular Oxidative Damage in LLC-PK1 Cells

SONG Jia-le

Department of Food Science and Nutrition, Pusan National University, Pusan 609-735, South Korea

Received:2012-06-04 Revised:2012-08-06 Online:2012-12-15 Published:2019-09-05

摘要/Abstract

摘要： 探讨茯砖茶乙醇提取物（FTE）对H₂O₂诱发猪肾近曲小管LLC-PK1上皮细胞氧化损伤的保护作用。以不同质量浓度（10~200μg/mL）的FTE预培养LLC-PK1细胞24h后,换用含500μmol/L H₂O₂的DMEM细胞培养液继续培养4h建立细胞氧化损伤模型。MTT法检测细胞生存率,硫代巴比妥酸比色法测定细胞内丙二醛（malondialdehyde, MDA）含量,比色法测定细胞内过氧化氢酶（catalase, CAT）,超氧化物歧化酶（superoxide dismutase, SOD）和谷胱甘肽过氧化物酶（glutathione peroxidase, GSH-px）含量。经不同浓度FTE预处理24h后,受损细胞的生存率上升,细胞内MDA生成量减少。同时,细胞内主要抗氧化酶（CAT、SOD和GSH-px）含量也较对照组增加,并呈剂量效应关系。结果提示,预先经FTE的保护可有效地对抗H₂O₂诱发的LLC-PK1细胞氧化损伤。

关键词: 茯砖茶, 氧化损伤, 抗氧化性, LLC-PK1细胞

Abstract: The aim of this study is to investigate the protective effect of ethanolic extract from Fuzhuan brick tea (FTE) on H₂O₂-induced oxidative damage in LLC-PK1 cells. Various concentration (10~200μg/mL) of FTE were pre-incubated with LLC-PK1 cells for 24h, and then exposed to H₂O₂(500μmol/L) for 4h. The cell viability was determined by MTT assay, contents of malondialdehyde (MDA) were determined by thiobarbituric acid reactive substances (TBARS) assay. Intracellular antioxidant enzyme activities, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were determined by colorimetric assay according to the protocols of the commercial kit. The cell viabilities of H₂O₂treated LLC-PK1 cells were increased, and the contents of MDA were decreased after 24h pre-incubated with different concentrations of FTE. In addition, the antioxidant enzymes (CAT, SOD and GSH-px) levels were also increased in FTE compared with H₂O₂ treated group. There was statistical significant difference between the FTE treated groups and untreated H₂O₂treated groups (all P<0.05). Those results suggested that pre-incubated with FTE showed a protective effect against H₂O₂-induced oxidative damage in LLC-PK1 cells.

Key words: Fuzhuan brick tea, oxidative damage, antioxidant, LLC-PK1 cells

中图分类号:

TS272.5⁺4

宋家乐. 茯砖茶对H₂O₂诱发LLC-PK1细胞损伤的保护作用[J]. 茶叶科学, 2012, 32(6): 539-542. doi: 10.13305/j.cnki.jts.2012.06.004.

SONG Jia-le. Protective Effect of Fuzhuan Brick Tea on H₂O₂-Induced Cellular Oxidative Damage in LLC-PK1 Cells[J]. Journal of Tea Science, 2012, 32(6): 539-542. doi: 10.13305/j.cnki.jts.2012.06.004.

[1]	代昕玥, 葛炳钢, 张旭雯, 刘文武, 段继春, 傅冬和. 茯砖茶改善2型糖尿病小鼠代谢紊乱的效果研究[J]. 茶叶科学, 2022, 42(1): 63-75.
[2]	黄翔翔, 谭婷, 禹利君, 王坤波, 黄建安, 徐诗钰, 刘仲华. 茯砖茶对葡聚糖硫酸钠诱导UC小鼠炎症及肠道微生物的影响[J]. 茶叶科学, 2021, 41(5): 681-694.
[3]	左高隆, 陈美艳, 林勇, 林海燕, 刘仲华, 吴文亮. 茯砖茶对NAFLD大鼠肠道黏膜屏障及肝损伤的保护作用研究[J]. 茶叶科学, 2019, 39(6): 631-640.
[4]	胡安恺, 姚立筠, 赵悦伶, 刘畅, 王岳飞, 徐平. 茯砖茶提取物对葡聚糖硫酸钠诱导小鼠炎症性肠病的保护作用研究[J]. 茶叶科学, 2019, 39(6): 641-651.
[5]	侯爱香, 颜道民, 孙静文, 郑旭, 李赛丹, 肖润花, 贝爽. 绿茶、红茶和茯砖茶水提物对肠道微生物体外发酵特性的影响[J]. 茶叶科学, 2019, 39(4): 403-414.
[6]	吴根梁, 侯爱香, 李珂, 李宗军. 陈年茯砖茶多酚类对老年人肠道菌群的影响研究[J]. 茶叶科学, 2018, 38(3): 319-330.
[7]	沈程文, 邓岳朝, 周跃斌, 齐冬晴, 贺群, 田双红, 林娟. 湖南茯砖茶品质特征及其香气组分研究[J]. 茶叶科学, 2017, 37(1): 38-48.
[8]	李适, 谌滢, 傅冬和, 刘仲华, 黄建安. 不同年份茯砖茶感官品质研究[J]. 茶叶科学, 2016, 36(5): 500-504.
[9]	赵仁亮, 谭吉慧, 卢秦华, 吴丹, 朱旗. 茯砖茶发花微生物生物学特性研究[J]. 茶叶科学, 2016, 36(2): 160-168.
[10]	贺国文, 钟桐生, 彭晓赟, 龙立平, 赵运林, 章卫民. 茯砖茶褐素组分特征及对α-葡萄糖苷酶活性抑制研究[J]. 茶叶科学, 2016, 36(1): 102-110.
[11]	吴香兰, 刘仲华, 曹丹, 金莉莎, 林勇. 茯砖茶对小鼠肠道免疫功能调节作用的研究[J]. 茶叶科学, 2013, 33(2): 125-130.
[12]	傅冬和, 刘仲华, 黄建安, 蔡汶莉. 茯砖茶降脂功能成分研究[J]. 茶叶科学, 2012, 32(3): 217-223.
[13]	王蝶, 黄建安, 叶小燕, 曹丹, 张健炜, 刘仲华. 茯砖茶减肥作用研究[J]. 茶叶科学, 2012, 32(1): 81-86.
[14]	关美玲, 刘仲华, 刘素纯, 陈栋, 黄建安, 李佳莲. 黑茶发花过程中冠突散囊菌对茶叶中游离氟含量的影响[J]. 茶叶科学, 2011, 31(5): 386-390.
[15]	徐小江, 黄建安, 肖力争, 袁勇, 肖文军. 茯砖茶及其配方对脂肪变性L-02肝细胞中TG含量的影响[J]. 茶叶科学, 2011, 31(3): 247-254.

茯砖茶对H₂O₂诱发LLC-PK1细胞损伤的保护作用

Protective Effect of Fuzhuan Brick Tea on H₂O₂-Induced Cellular Oxidative Damage in LLC-PK1 Cells

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10