聚乙二醇胁迫下茶树叶片的蛋白质组分析

doi:10.13305/j.cnki.jts.2009.2.001

茶叶科学 ›› 2009, Vol. 29 ›› Issue (2): 79-88.doi: 10.13305/j.cnki.jts.2009.2.001

• • 下一篇

聚乙二醇胁迫下茶树叶片的蛋白质组分析

郭春芳^1,2, 孙云³, 赖呈纯³, 张木清^1,*

1. 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州 350002;
2. 福建教育学院,福建福州 350025;
3. 福建农林大学园艺植物生物工程研究所,福建福州 350002

收稿日期:2008-07-25 修回日期:2008-11-07 出版日期:2009-04-15 发布日期:2019-09-06
通讯作者: ^* zmuqing@163.com
作者简介:郭春芳（1965— ）,男,福建厦门人,博士,副教授,主要从事植物生理与分子生物学研究。
基金资助:
福建省自然科学基金资助项目（B0510025）和福建省教育厅科技计划资助项目（JA05333）

Proteomic Analysis of Tea Leaf under Polyethylene Glycol Stress

GUO Chun-fang^1,2, SUN Yun³, LAI Cheng-chun³, ZHANG Mu-qing^1,*

1. Key Laboratory of Eco-physiology and Genetic Improvement for Sugarcane under Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Fujian Institute of Education, Fuzhou 350025, China;
3. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2008-07-25 Revised:2008-11-07 Online:2009-04-15 Published:2019-09-06

摘要/Abstract

摘要： 应用差异蛋白质组学方法分析了铁观音茶树幼苗在聚乙二醇（Polyethylene Glycol,PEG）胁迫下叶片蛋白质组的变化。对18个蛋白质点在PEG胁迫下的变化特点进行了分析,并对有差异表达的16个蛋白质点进行了质谱分析,共鉴定出14个蛋白质点,代表了10种不同的蛋白,其中5个点是同一个蛋白(1,5-二磷酸核酮糖羧化酶/加氧酶大亚基,RubisoLSU),另9个蛋白是PEG胁迫响应蛋白,包括光合系统II 23 kDa多肽、叶绿体伴侣蛋白21(ch-Cpn21)、磷酸丙糖异构酶(TPI)、三磷酸腺苷合酶β亚基、S-腺苷甲硫氨酸合成酶（SAMS1）、磷脂酰乙醇胺结合蛋白(CEN)、新生多肽相关复合物α亚基(α-NAC)、20 S蛋白酶体α亚基(PAE2)、翻译起始因子5A(eIF5A)等,这些蛋白质分别参与了叶绿体组成、糖代谢、能量代谢、硫代谢、蛋白质代谢、信号转导、基因表达调节和细胞程序性死亡等生命活动过程。

关键词: 茶树, 聚乙二醇胁迫, 蛋白质组, 双向电泳, 质谱

Abstract: Two-dimensional electrophoresis(2-DE) were carried out to study the proteomic change in of tea leaves in responsive to polyethylene glycol(PEG) stress, and expression profiles of 18 different proteins were analyzed under PEG stress. Sixteen different proteins were identified by MS/MS using 4700 Proteomics Analyzer(Applied Biosystems, USA),and 14 proteins were identified and represented 10 kinds of proteins. In these proteins, 5 were the same protein—ribulose 1, 5-bisphosphate carboxylase/oxygenase large subunit(RubisoLSU), other 9 proteins were response proteins to PEG stress including photosystem II 23 kDa polypeptide,chloroplast chaperonin 21, triose phosphate isomerase(TPI), ATP synthase subunit, S-adenosylmethionine synthetase 1(SAMS1), centroradialis(CEN), nascent polypeptide associated complex alpha chain(α-NAC), 20 S proteasome alpha subunit E2(PAE2) and translation initiation factor 5A(eIF5A). These proteins will play an important role in many life activities of plant, such as chloroplast composition, sugar metabolism, energy metabolism, sulfur metabolism, protein metabolism, signal transduction, gene expression regulation and programmed cell death.

Key words: tea(Camellia sinensis), PEG stress, proteome, two-dimensional electrophoresis(2-DE), mass spectrometry

中图分类号:

S571.1
Q51

郭春芳, 孙云, 赖呈纯, 张木清. 聚乙二醇胁迫下茶树叶片的蛋白质组分析[J]. 茶叶科学, 2009, 29(2): 79-88. doi: 10.13305/j.cnki.jts.2009.2.001.

GUO Chun-fang, SUN Yun, LAI Cheng-chun, ZHANG Mu-qing. Proteomic Analysis of Tea Leaf under Polyethylene Glycol Stress[J]. Journal of Tea Science, 2009, 29(2): 79-88. doi: 10.13305/j.cnki.jts.2009.2.001.

参考文献

[1] O'Farrell PH.High-resolution 2-dimensional electrophoresis of proteins[J]. J Biol Chem, 1975, 250: 4007~4021.
[2] Jiang Y, Huang B.Protein alterations in response to water stress and ABA in Tall fescue[J]. Crop Science, 2002, 42(1): 202~207.
[3] 张洁, 谢惠民, 吕树作, 等. 水分胁迫条件下冬小麦幼苗应答蛋白的表达及其与品种抗旱性的关系[J]. 麦类作物学报, 2007, 27(2) :303~308.
[4] Nover L, Bharti K, Doring P, et al. Arabidopsis and the heat stress transcription factor world: how many heat stress transcriptionfactors do we need?[J]. Cell Stress Chaperones, 2001, 6(3): 177~189.
[5] 朱友林, 吴健胜, 王金生. 水稻对白叶枯病菌抗性相关蛋白的双向电泳分析[J]. 中国农业科学, 2000, 33(4): 91~93.
[6] 霍晨敏, 赵宝存, 葛荣朝, 等. 小麦耐盐突变体盐胁迫下的蛋白质组分析[J]. 遗传学报, 2004, 31(12): 1408~1414.
[7] Franklin E C, Johnie N J, Roy G C, et al. Changes in cotton root proteins correlated with resistance to root knot nematode development[J]. The Journal of Cotton Science, 1997, 1: 38~47.
[8] 林金科, 郑金贵, 袁明, 等. 外源诱导提高茶树EGCG含量过程的蛋白质组差异分析[J]. 茶叶科学, 2005,25(2): 109~115.
[9] Bradford MM.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 1976, 72: 248~254.
[10] 郭尧君. 蛋白质电泳实验技术[M]. 北京: 科学出版社, 2005: 107~108.
[11] Serdler A.The extrinsic polypeptides of photosystem II[J]. Biochim Biophys Atca, 1996, 1277: 35~60.
[12] Laudenbach DE, Herbert SK, McDowell C, et al. Cytochrome e-553 is not required for photosynthetic activity in the cyanobacterum Synechococcus[J]. Plant Cell, 1990, 2: 913~924.
[13] Riccardi F, Gazeau P, Vienne D, et al. Protein changes in response to progressive water deficit in maize: Quantitative variation and polypeptide identification[J]. Plant Physiol, 1998, 117: 1253~1263.
[14] Tezara W, Mitchell V J, Driscoll S D, et al. Water stress inhibits plantphotosynthesis by decreasing coupling factor and ATP[J]. Nature, 1999, 401(6756): 914~917.
[15] Zhao CF, Wang JQ, Cao ML, et al. Proteomic changes in rice leaves during development of field-grown rice plants[J]. Proteomics, 2005, 5: 961~972.
[16] Desimone M, Henke A, Wagner E.Oxidative stress induces partial degradation of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase in isolated chloroplasts of barley[J]. Plant Physiol, 1996, 111: 789~796.
[17] 蔡秋华, 张积森, 郭春芳, 等. 高等植物体内多胺的生理功能及其分子生物学研究进展[J]. 福建教育学院学报, 2006, 7(10): 118~124.
[18] Mayne M B, Coleman J R, Bluwald E.Differential expression during drought conditioning of a root-specfic S-adnosyl methionine synthetase from jack pine (Pinus banksiasna Lamb.) seedling[J]. Plant Cell Environ, 1996, 19: 958~966.
[19] Nonami H,Boyer J S.Wall extensibility and cell hydraulic conductivity decrease in enlarging stem tissues at low water potentials[J]. Plant physiol, 1990, 93: 1610~1619.
[20] Bonfield M J, Barker J J, Perry A C, et al. Function from structure? The crystal structure of human phosphatidylethano-lamine-binding protein suggests a role in membrane signal transduction[J]. Structure, 1998, 6: 1245~1254.
[21] Gibbons R, Adeaya-Osiguwa S A, Fraser L R. A mouses sperm decapacilation factor receptor is phosphatidylethanolemine- binding protein 1[J]. Reproduction, 2005, 130(4): 497~508.
[22] Reimann B, Bradsher J, Franke J, et al. Initial characterisation of the nascent polypeptide- associated complex in yeast[J]. Yeast, 1999: 15: 397~407.
[23] Rospert S, Dubaquie Y, Gautschi M.Nascent- polypeptide- associated complex[J]. Cell Mol Life Sci, 2002, 59: 1632~1639.
[24] 刘建祥. 干旱诱导水稻雄性不育的生理和蛋白质组学研究[D]. 杭州: 浙江大学, 2003.
[25] Wang TW,Lu L, Ghang CG, et al. Pleiotropic effects of suppressing deoxyhypusinesynthase expression in Arabidopsis thaliana[J]. Plaat Mol Biol, 2003, 52: 1223~1235.
[26] Xu A, Jao DL, Chen KY.Indentification of mRNA that binds to eukaryotic initiation factor 5A by affinity co-purification and differential display[J]. Biochem J, 2004, 384: 585~590.
[27] Moyle R, Fairhairn DJ, Ripi J, et al. Developing pineapple fruit has a small transcriptome dominated by metallothionein[J]. J Exp Bot, 2005, 409: 101~112.

聚乙二醇胁迫下茶树叶片的蛋白质组分析

Proteomic Analysis of Tea Leaf under Polyethylene Glycol Stress

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