传统SMB、Varicol和Partial-discard工艺分离纯化ECG和EGCG的比较研究

doi:10.13305/j.cnki.jts.2011.03.007

茶叶科学 ›› 2011, Vol. 31 ›› Issue (3): 201-210.doi: 10.13305/j.cnki.jts.2011.03.007

传统SMB、Varicol和Partial-discard工艺分离纯化ECG和EGCG的比较研究

黄永东^1,2,3, 江和源^1,2*, 江用文^1,2, 张建勇^1,2, 王斌^1,2,3, 白艳^1,2,3, 文志浩^1,2,3, 王伟伟^1,2,3

1. 中国农业科学院茶叶研究所,农业部茶及饮料植物产品加工与质量控制重点开放实验室,浙江杭州 310008;
2. 浙江省茶叶加工工程重点实验室,浙江杭州 310008;
3. 中国农业科学院研究生院,北京 100081

收稿日期:2010-12-02 修回日期:2011-01-14 出版日期:2011-06-15 发布日期:2019-09-06
通讯作者: jianghy@mail.tricaas.com
作者简介:黄永东（1987— ）,男,硕士研究生,主要从事天然产物化学、茶叶深加工等研究。
基金资助:
国家948项目（2011-Z10）、农业部留学基金和教育部留学基金

Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography

HUANG Yong-dong^1,2,3, JIANG He-yuan^1,2*, JIANG Yong-wen^1,2, ZHANG Jian-yong^1,2, WANG Bin^1,2,3, BAI Yan^1,2,3, WEN Zhi-hao^1,2,3, WANG Wei-wei^1,2,3

1. Tea Reasearch Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea and Beverage Crop Product Processing and Quality Control, Agriculture Ministry, Hangzhou 310008, China;
2. Key Laboratory of Tea Processing Engineering of Zhejiang Province, Hangzhou 310008, China;
3. Graduate School of Chinese Academy of Agriculture Sciences, Beijing 100081, China

Received:2010-12-02 Revised:2011-01-14 Online:2011-06-15 Published:2019-09-06

摘要/Abstract

摘要： 使用B型三带模拟移动床技术,以C₁₈键合硅胶为固定相,乙醇与水（20︰80,V/V）为流动相,分别采取传统SMB工艺、Varicol工艺和Partial-discard工艺分离98%的茶多酚（TP98）。以得到高纯度和高回收率的ECG和EGCG为目标,分别优化柱配置、切换时间、进样流速、冲洗流速。结果表明,采用Varicol工艺能得到纯度为91.33%的ECG,回收率为91.41%;采用Partial-discard工艺能得到纯度为90.12%的EGCG,回收率为97.83%。相对于传统模拟移动床,Varicol工艺具有提高产品纯度和回收率的优点,Partial-discard工艺具有提高收集液浓度和产品纯度的优点。

关键词: 模拟移动床, EGCG, ECG, Varicol工艺, Partial-discard工艺

Abstract: With C₁₈ bonded silica as stationary phase, ethanol and water (20︰80, V/V) as mobile phase, tea polyphenols were separated by traditional Simulated Moving Bed, Varicol process and Partial-discard process. Column configuration, switching time, feed flow rate, elution flow rate were optimized respectively in order to obtain epicatechin gallate (ECG) and epigallocatechin gallate(EGCG)with high purity and high recovery. Varicol process could be employed to separate 98% green tea polyphenols (TP98) to obtain ECG with 91.33% purity and 91.41% recovery. Partial-discard process could be adopted to separate TP98 to obtain EGCG with 90.12% purity and 97.83% recovery. Compared with traditional process, Varicol process has the advantages of higher purity and recovery of target product, while Partial-discard process shows the advantages of increasing the concentration of raffinate and the purity of target product.

Key words: simulated moving bed, EGCG, ECG, varicol, partial-discard

中图分类号:

TS272.4

黄永东, 江和源, 江用文, 张建勇, 王斌, 白艳, 文志浩, 王伟伟. 传统SMB、Varicol和Partial-discard工艺分离纯化ECG和EGCG的比较研究[J]. 茶叶科学, 2011, 31(3): 201-210. doi: 10.13305/j.cnki.jts.2011.03.007.

HUANG Yong-dong, JIANG He-yuan, JIANG Yong-wen, ZHANG Jian-yong, WANG Bin, BAI Yan, WEN Zhi-hao, WANG Wei-wei. Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography[J]. Journal of Tea Science, 2011, 31(3): 201-210. doi: 10.13305/j.cnki.jts.2011.03.007.

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传统SMB、Varicol和Partial-discard工艺分离纯化ECG和EGCG的比较研究

Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography

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