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茶叶科学 ›› 2010, Vol. 30 ›› Issue (5): 336-342.doi: 10.13305/j.cnki.jts.2010.05.002

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普洱熟茶和生茶香气成分的提取和测定分析

洪涛1, 黄遵锡1,2,3,4, 李俊俊1,2,3, 唐湘华1,2,3,4, 慕跃林1,2,4, 许波1,2,3,4,*   

  1. 1.云南师范大学生命科学学院,云南 昆明 650092;
    2. 生物能源持续开发利用教育部工程研究中心,云南 昆明 650092;
    3. 云南省生物质能与环境生物技术重点实验室,云南 昆明 650092;
    4. 云南师范大学酶工程重点实验室,云南 昆明 650092
  • 收稿日期:2010-01-27 修回日期:2010-07-14 发布日期:2019-09-11
  • 作者简介:洪涛(1986— ),男,湖北武汉人,在读硕士研究生,主要从事普洱茶渥堆发酵方面的研究。
  • 基金资助:
    云南省社会发展科技计划应用基础研究专项(2008CD105)

Extraction and Analysis on the Aromatic Components of Pu-erh Ripe Tea and Raw Tea

HONG Tao1, HUANG Zun-xi1,2,3,4, LI Jun-jun1,2,3, TANG Xiang-hua1,2,3,4, MU Yue-lin1,2,4, XU Bo1,2,3,4,*   

  1. 1. School of Life Sciences, Yunnan Normal University, Kunming 650092, China;
    2. Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650092, China;
    3. Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650092, China;
    4. Key Laboratory of Enzymatic Engineering, Yunnan Normal University, Kunming 650092, China
  • Received:2010-01-27 Revised:2010-07-14 Published:2019-09-11

摘要: 以不同年份出品的普洱熟茶和生茶为研究对象,采用减压蒸馏萃取法(VDE),并用二氯甲烷和无水乙醚对各茶中香气成分进行提取,最后用GC-MS分析各年份茶中的香气成分。结果表明,不论是熟茶还是生茶,用二氯甲烷提取到的香气种类均要多于无水乙醚。从提取效果来看,二氯甲烷对碳氢化合物、杂氧化合物、含氮化合物、酮类香气物质的提取效果较好,而无水乙醚对酯类和酚类香气物质有较好的提取效果。熟茶在储存陈化过程中苯并噻唑和咖啡碱含量会增高,而邻苯二甲酸-异丁基-辛基酯和1,2,4-三甲氧基苯含量会降低。生茶在储存陈化过程中5-氨基-1-乙基吡唑、N-苯基-1-萘胺、1,2,3-三甲氧基苯和邻苯二甲酸-异丁基-辛基酯含量会增高,而(反式)角鲨烯、芳樟醇、β-异甲基紫罗兰酮和咖啡碱含量会降低。同时,熟茶中杂氧化合物类香气种类较多,主要有以1,2,3-三甲氧基苯为主的10种杂氧化合物。生茶中醇类香气物质则较熟茶丰富,主要有1-辛烯-3-醇、芳樟醇、2,6-二甲基环己醇、苯乙醇、雪松醇。

关键词: 普洱熟茶, 普洱生茶, 二氯甲烷, 无水乙醚, GC-MS分析

Abstract: The aromatic components from Pu-erh ripe and raw tea which storaged for different years extracted by methylene chloride and ethyl ether through vacuum distillation extraction, and then investigated by GC-MS. It showed that the aromatic compounds extracted by methylene chloride were more than ethyl ether no matter they were ripe tea or raw tea. Those compounds including hydrocarbons, heterocyclic oxygen compounds, nitrogenous compounds, ketones were extracted more by methylene chloride than those extracted by ethyl ether. However, more esters and phenolic compounds were extracted by ethyl ether. The contents of benzothiazole and caffeine increased during the process of ripe tea’s storage, but the contents of phthalic acid, isobutyl octyl ester and 1,2,4-trimethoxybenzene were decreased. The contents of 5-Amino-1-ethylpyrazole, N-phenyl-1-naphthalenamine, 1,2,3-trimethoxybenzene and phthalic acid-iso-butyl-octyl ester were increased during the storaging process of raw tea, but the contents of trans-squalene, linalool, β-iso-methyl ionone and caffeine were decreased. At the same time, there were many aromatic compounds of heterocyclic oxygen compounds in ripe tea. The main components of these 10 heterocyclic oxygen compounds included 1,2,3-trimethoxybenzene. The contents of alclhols aromatic components in raw tea, such as 1-octen-3-yl, linalool, cyclohexanol, 2,6-dimethylcyclohexanol, phenethyl alcohol and cedrol.

Key words: Pu-erh ripe tea, Pu-erh raw tea, methylene chloride, ethyl ether, GC-MS analysis

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