低磷胁迫下茶树根系CS基因的克隆及表达分析

doi:10.13305/j.cnki.jts.2010.05.006

摘要/Abstract

摘要： 柠檬酸合成酶（Citrate Synthase, CS）是茶树体内合成柠檬酸的关键性酶。克隆了茶树根系柠檬酸合成酶基因片段,GenBank登录号为FJ814766,且利用半定量RT-PCR方法,研究该基因在低磷下的表达变化。结果表明,当供磷浓度为0（缺磷）时,根系CS基因的表达略有增强,这可能是茶树对低磷的一种适应机制。

关键词: 半定量RT-PCR, 低磷胁迫, 基因表达, 克隆, 茶树

Abstract: Citrate synthase(CS) is the key enzyme of producing citrate acid. CS gene fragments were cloned from tea roots. The GenBank accession is FJ814766 in the NCBI. Then the changes in gene expression in response to P supply were investigated by using semi-quantitative RT-PCR. The result showed that P deficiency increased the expressions of CS gene. This may be a tolerant mechanism of tea seedlings to P deficiency.

Key words: semi-quantitative RT-PCR, low P stress, gene expression, cloning, tea plant

中图分类号:

S571
Q523

林郑和, 陈常颂, 邬龄盛. 低磷胁迫下茶树根系CS基因的克隆及表达分析[J]. 茶叶科学, 2010, 30(5): 362-366. doi: 10.13305/j.cnki.jts.2010.05.006.

LIN Zheng-he, CHEN Chang-song, WU Ling-sheng. Cloning and Expression Analysis on Citrate Synthase Gene of Tea Root under Low Phosphorous Stress[J]. Journal of Tea Science, 2010, 30(5): 362-366. doi: 10.13305/j.cnki.jts.2010.05.006.

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