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茶叶科学 ›› 2010, Vol. 30 ›› Issue (6): 475-481.doi: 10.13305/j.cnki.jts.2010.06.012

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普洱茶提取物抗氧化及其对Na2S2O3诱导HUVEC损伤的保护作用

王岳飞1, 罗子华1, 邬新荣2, 陈红波1,*, 徐平1, 杨贤强1   

  1. 1. 浙江大学茶学系,浙江 杭州 310029;
    2. 浙江省茶叶集团有限公司,浙江 杭州 310016
  • 收稿日期:2010-03-08 修回日期:2010-08-19 发布日期:2019-09-11
  • 通讯作者: chenhbo212@163.com
  • 作者简介:王岳飞(1968— ),男,浙江天台人,博士,主要从事茶叶生物化学与茶终端产品研究开发。

Antioxidtive Activities of Pu-erh Tea Extracts and Their Protective Effect on HUVEC Damage Induced by Na2S2O3

WANG Yue-fei1, LUO Zi-hua1, WU Xin-rong2, CHEN Hong-bo1,*, XU Ping1, YANG Xian-qiang1   

  1. 1. Department of Tea Science, Zhejiang University, Hangzhou 310029, China;
    2. Zhejiang Tea Group CO.LTD, Hangzhou 310016, China
  • Received:2010-03-08 Revised:2010-08-19 Published:2019-09-11

摘要: 利用普洱茶加工过程中废弃的碎茶灰为原料制备提取物,分析其主要化学成分组成,以ABTS和FRAP体系评价了普洱生茶、普洱熟茶提取物的抗氧化活性,并研究了这两种提取物对Na2S2O3诱导人脐静脉血管内皮细胞(HUVEC)损伤的保护作用。结果表明,普洱熟茶提取物中主要化学成分除咖啡碱外均低于普洱生茶提取物,其中酯型儿茶素含量减少最多。同时,普洱熟茶提取物对ABTS+清除能力和铁离子还原能力都较普洱生茶提取物作用弱,而对HUVEC损伤的保护作用却强于普洱生茶提取物,并在特定浓度下与茶多酚相当。

关键词: 普洱茶, 抗氧化, HUVEC, 保护作用

Abstract: Extracts of raw and ripe Pu-erh tea were prepared from tea dust during processing. The antioxidtive activities of extracts were evaluated by ABTS and FRAP systems. The potentially protective effects of extracts on human umbilical vascular endothelial cells(HUVEC) were investigated in Na2S2O3-induction model. The results showed that the extract of raw Pu-erh tea had stronger antioxidtive activity than that of ripe Pu-erh tea in both test systems. But extract of ripe Pu-erh tea showed a less protective effect than that of extract of raw Pu-erh tea on the HUVEC cell damage induced by Na2S2O3, and relevent with tea polyphenols under certain concentrations.

Key words: Pu-erh tea, antioxidant, HUVEC, protective effect

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