表没食子儿茶素没食子酸酯增强卡莫司汀抗肿瘤作用的体外研究

doi:10.13305/j.cnki.jts.2007.04.007

茶叶科学 ›› 2007, Vol. 27 ›› Issue (4): 302-306.doi: 10.13305/j.cnki.jts.2007.04.007

表没食子儿茶素没食子酸酯增强卡莫司汀抗肿瘤作用的体外研究

黄秀华¹, 张丹², 郝津², 杨足君¹, 任正隆^{1, *}

1. 电子科技大学生命科学与技术学院分子生物学实验室,四川成都 610051;
2. 成都恩威集团四川恩威中医药研究开发有限公司药理室,四川成都,610041

收稿日期:2007-05-24 修回日期:2007-08-27 出版日期:2007-12-25 发布日期:2019-09-11
通讯作者: *任正隆,renzl@uestc.edu.cn
作者简介:黄秀华（1970— ）,女,重庆梁平人,博士生,从事抗菌抗肿瘤和基因毒性方面研究。
基金资助:
电子科技大学杰出人才引进项目JC901002

(-)-epigallocatechin Gallate (EGCG) Enhance the Anticancer Activity Induced by Carmustine in vitro

HUANG Xiu-hua¹, ZHANG Dan², HAO Jin², YANG Zu-jun¹, REN Zheng-long^{1, *}

1. The Laboratory of Molecular Biology, College of Life Sicence and Technology, University of Electronic Science and Technology, Chengdu 610051, China;
2. The Laboratory of Pharmacology, Sichuan Enwei Institute of Traditional Chinese Medicine, Enwei Group, Chengdu 610041, China

Received:2007-05-24 Revised:2007-08-27 Online:2007-12-25 Published:2019-09-11

摘要/Abstract

摘要： 采用MTT法及Comet assay(彗星实验分析方法)研究了表没食子儿茶素没食子酸酯（EGCG）与卡莫司汀联用对人肺癌细胞A549的生长抑制率及DNA损伤的影响,结果表明：联合应用EGCG能降低卡莫司汀对人肺癌细胞A549的IC₅₀。卡莫司汀单用对人肺癌细胞A549的IC₅₀为187.46βμg/ml,当用无毒浓度25βμg/ml和50βμg/ml的EGCG与卡莫司汀联用时,卡莫司汀对人肺癌细胞A549的IC₅₀分别下降为139.56βμg/ml和154.02βμg/ml。EGCG还能增强卡莫司汀引起的人肺癌细胞A549 DNA的损伤,彗星尾矩值由单用时的19.02±14.60上升为联用时的33.07±10.47。结果说明EGCG能增强卡莫司汀对人肺癌细胞A549造成的DNA损伤,从而增强其抗肿瘤活性。

关键词: EGCG, 卡莫司汀, DNA损伤, 彗星实验, IC₅₀

Abstract: The effect of (-)-epigallocatechin gallate (EGCG) combined with carmustine on the growth inhibition and DNA damage of human lung carcinoma cell line A549 were evaluated by MTT assay and Comet assay repectively. The results showed that 50% inhibitory concentration (IC₅₀) of carmustine to A549 human lung carcinoma cell line was decreased when combined with EGCG. The 50% inhibitory concentration (IC₅₀) of carmustine alone to human lung carcinoma cell line A549 was 187.46βμg/ml, When the carmustine is combine with EGCG(25βμg/ml and 50βμg/ml, the concentration under which EGCG has no growth inhibition to A549), however, the IC₅₀ value to A549 cell line decreased to 139.56βμg/ml and 154.02βμg/ml respectively. EGCG also enhanced carmustine-induced DNA damage in A549 cell line by Comet assay. The tail moment increased from 19.02±14.60 to 33.07±10.47. So, EGCG can enhance carmustine-induced DNA damage in A549 cell line, hence enhance the anticancer activity of carmustine.

Key words: EGCG, carmustine, DNA damage, comet assay, IC₅₀

中图分类号:

黄秀华, 张丹, 郝津, 杨足君, 任正隆. 表没食子儿茶素没食子酸酯增强卡莫司汀抗肿瘤作用的体外研究[J]. 茶叶科学, 2007, 27(4): 302-306. doi: 10.13305/j.cnki.jts.2007.04.007.

HUANG Xiu-hua, ZHANG Dan, HAO Jin, YANG Zu-jun, REN Zheng-long. (-)-epigallocatechin Gallate (EGCG) Enhance the Anticancer Activity Induced by Carmustine in vitro[J]. Journal of Tea Science, 2007, 27(4): 302-306. doi: 10.13305/j.cnki.jts.2007.04.007.

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表没食子儿茶素没食子酸酯增强卡莫司汀抗肿瘤作用的体外研究

(-)-epigallocatechin Gallate (EGCG) Enhance the Anticancer Activity Induced by Carmustine in vitro

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