茶树ACC氧化酶基因全长cDNA的克隆与表达分析

doi:10.13305/j.cnki.jts.2008.06.006

摘要/Abstract

摘要： ACC(1-氨基环丙烷-1-羧酸)氧化酶是植物乙烯合成过程中的关键酶之一,对乙烯的合成具有重要的调控作用。在茶树新梢cDNA文库测序所获得ESTs基础上,利用RT-PCR技术,克隆得到编码ACC氧化酶基因的全长序列,GenBank登录号为DQ904328。该基因长1 232 bp,编码320个氨基酸残基,预测分子量为36.2 kD,等电点5.41。多序列联配表明茶树ACC氧化酶具有高度保守区域,基于邻接法的进化树显示与柿树ACC氧化酶的亲缘关系最近。对经高、低温后的不同品种进行RT-PCR分析,结果发现ACC氧化酶基因的表达量与品种的抗逆性有一定的相关性。

关键词: 茶树, 乙烯, ACC氧化酶, 基因克隆, 表达分析

Abstract: 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase is an important enzyme and plays a critical regulatory role in the biosynthesis of ethylene. Based on previous ESTs sequencing results of the tender shoots cDNA library and RT-PCR technology, a full-length cDNA sequence coding ACC oxidase (ACO) of tea plant was cloned. The GenBank accession is DQ904328 in the NCBI. The ACO gene had 1 232 bp in length, encoding 320 amino acid residues with the putative molecular weight of 36.2 KD and the pI 5.41. The protein sequences of tea plant ACO aligned with those of other 12 plants showed highly conservative sequences in tea plant. Neighbor-Joining phylogenetic tree based on ACO sequence of tea plant and other 22 plants indicated that tea plant had very close relationship with that of Diospyros kaki. The ACO gene expression level of different cultivars after high and low temperature stresses were analyzed using RT-PCR method. The expression level has correlation with resistance of the cultivars to certain degree.

Key words: tea plant (Camellia sinensis), ethylene, ACC oxidase, gene cloning, expression analysis

中图分类号:

S571.1
Q523

张亚丽, 乔小燕, 陈亮. 茶树ACC氧化酶基因全长cDNA的克隆与表达分析[J]. 茶叶科学, 2008, 28(6): 459-467. doi: 10.13305/j.cnki.jts.2008.06.006.

ZHANG Ya-li, QIAO Xiao-yan, CHEN Liang. Molecular Cloning and Expression Analysis on ACC Oxidase Gene Full-length cDNA from Tea Plant[J]. Journal of Tea Science, 2008, 28(6): 459-467. doi: 10.13305/j.cnki.jts.2008.06.006.

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