丝网印刷碳电极传感器检测茶叶中痕量铅研究

doi:10.13305/j.cnki.jts.2008.02.005

茶叶科学 ›› 2008, Vol. 28 ›› Issue (2): 93-100.doi: 10.13305/j.cnki.jts.2008.02.005

丝网印刷碳电极传感器检测茶叶中痕量铅研究

赵广英, 吴艳燕

浙江工商大学食品与生物工程学院,浙江省食品安全重点实验室杭州 310035

收稿日期:2007-07-25 修回日期:2007-12-07 出版日期:2008-04-15 发布日期:2019-09-16
作者简介:赵广英（1960–）,女,教授,研究方向为食品质量安全快速检测,食品卫生微生物检验,E-mail：zhaogy-user@163.com
基金资助:
国家自然科学基金（No.30571623）; 浙江省重中之重学科(No. Z05-29); 浙江省食品质量安全重点实验室开放项目（No. Z04-179）

Determination of Trace Lead in Tea with Screen-printed Carbon Electrodes

ZHAO Guang-ying, WU Yan-yan

Food safety key lab of Zhejiang, College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035

Received:2007-07-25 Revised:2007-12-07 Online:2008-04-15 Published:2019-09-16

摘要/Abstract

摘要： 建立了同位镀汞法修饰的丝网印刷碳电极电化学方波溶出伏安法快速检测茶叶中的铅的方法。详细描述了建立该系统检测方法的条件优化过程,得到了一系列最佳参数：最佳的镀汞液浓度4×10^-4 mol/L,沉积电位-1.1 V,电位增量3 mV,方波频率10 Hz,方波幅度0.05 V,pH值5,沉积时间280 s,平衡时间30 s。在优选条件下获得的方法灵敏度、线性范围和检测限分别为22.7 nA/(μg/L),10~225 μg/L (r=0.9986)和0.74 μg/L(S/N=3)。研究了对多种重金属元素存在条件下120 μg/L的铅检测的干扰情况。本试验对样品前处理方法研究发现,采用家用微波炉、聚四氟乙烯密封增压微波消化罐消化茶叶,用标准加入法对分析样品进行检测,与国标方法相比较,无显著偏差。该方法灵敏、准确、快速适合于茶叶中痕量铅的测定。

关键词: 方波溶出伏安法, 丝网印刷碳电极, 茶叶

Abstract: The square wave anodic stripping voltammetric (SWASV) methodology using a screen printed carbon electrode (SPCE) in situ plated in mercury (Ⅱ) was developed by the authors to determine lead in tea. A detailed description of the systematic laboratory test carried out to optimize its performance is reported. A series of optimum parameter are as follows Hg²⁺concentration: 4×10^-4 mol/L, Plating potential: -1.1 V, E_step: 3 mV, Frequency: 10 Hz, Amplitude: 60 mV, pH: 5, plating time: 280 s, balance time: 30 s. Under the optimized analytical condition, the obtained sensitivity, linearity, and detection limit are 22.7 nA/(μg/L), 10~225 μg/L (r=0.9986), and 0.74 μg/L(S/N=3),respectively. The interference effect was thoroughly studied with various metals in the determination of 120 μg/L Pb²⁺. Tea was digested using domestic microwave oven and PTFE sealed and pressurized microwave digestion tank. The standard method of analysis was used to detect the samples and comprised with the GB method, no significant deviation was found. The method was sensitive, accurate, fast and suitable for the determination of trace lead in tea.

Key words: square wave anodic stripping voltammetry, screen printed carbon electrode, tea

中图分类号:

TS272
O657.8

赵广英, 吴艳燕. 丝网印刷碳电极传感器检测茶叶中痕量铅研究[J]. 茶叶科学, 2008, 28(2): 93-100. doi: 10.13305/j.cnki.jts.2008.02.005.

ZHAO Guang-ying, WU Yan-yan. Determination of Trace Lead in Tea with Screen-printed Carbon Electrodes[J]. Journal of Tea Science, 2008, 28(2): 93-100. doi: 10.13305/j.cnki.jts.2008.02.005.

参考文献

[1] 陈天金, 魏益民, 潘家荣.食品中铅对人体危害的风险评估[J]. 中国食物与营养, 2007, 2: 15~18.
[2] 姜红艳, 龚淑英. 茶叶中铅含量现状及研究动态[J]. 茶叶, 2004, 30(4): 220~222.
[3] 励建荣, 陆海霞, 季静冰. 浙江省部分地区绿茶中重金属含量的调查和研究[J]. 中国食品学报, 2004,(01):88~89.
[4] 傅明, 袁智能, 黄志强, 等. 火焰原子吸收光谱法测定茶叶中的铅[J]. 光谱实验室, 2002(01): 65~67.
[5] Subbiah Seenivasan, Natarajan Manikandan, Narayanan Nair Muraleedharan, et al. Heavy metal content of black teas from south India[J]. Food Control Heavy metal content of black teas from south India[J]. Food Control, 2007, Available online.
[6] M D Ioannidou, G A Zachariads, A N Anthemidis, et al. Direct determination of toxic trace metals in honey and sugars using inductively coupled plasma atomic emission sectrometry[J]. Talanta 2005, (65): 92~97.
[7] Zaijun Li, Jian Tang, Jiaomai Pan.The deter- mination of lead in preserved food by spectropho- tometry with dibromo hydroxyphenyl porphyrin[J]. Food Control, 2004(15): 565~570.
[8] Eric Fischer, Constant M.G, van den Berg. Anodic stripping voltammetry of lead and cadmium using a mercury film electrode and thiocyanate[J]. Analytica Chimica Acta, 1999, 385(1~3): 273~280.
[9] Slavka Stankovic, Dragana Čičkarić, Jelena Marković.Determination of Pb and Cd in water by potentiometric stripping analysis (PSA)[J]. Desali- nation, 2007, 213(1~3): 282~287.
[10] E. Shams, H. Abdollahi, M. Yekehtaz, et al. H-point standard addition method in the analysis by differential pulse anodic stripping voltammetry: Simultaneous determination of lead and tin[J]. Talanta, 2004, 63(2, 28): 359~364.
[11] Helena M. Carapuça, Sandra C.C. Monterroso et al. Simultaneous determination of copper and lead in seawater using optimised thin-mercury film electrodes in situ plated in thiocyanate media[J]. Talanta, 2004, 64(2,8): 566~569.
[12] Sandra C.C. Monterroso, Helena M. Carapuca, João E. J. Simão et al. Optimisation of mercury film deposition on glassy carbon electrodes: evaluation of the combined effects of pH, thiocyanate ion and deposition potential[J]. Analytica Chimica Acta, 2004, 503(2,9): 203~212.
[13] GB-T5009.12–2003 食品中铅的测定[S].
[14] 许钢. 微波消解──原子吸收法测定植物中的微量金属元素[J]. 无锡轻工大学学报, 1999(02): 41~46.
[15] 孔祥虹, 李建华, 李蓉. 微波消化技术在蔬菜微量元素测定中的应用[J]. 理化检验.化学分册, 2004(02): 91~92.

丝网印刷碳电极传感器检测茶叶中痕量铅研究

Determination of Trace Lead in Tea with Screen-printed Carbon Electrodes

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