质子泵在不同氮素形态调控茶树磷素吸收的功能研究

doi:10.13305/j.cnki.jts.2019.06.011

摘要/Abstract

摘要： 磷是植物生长发育的重要矿质营养元素之一,不同氮素形态均影响植物对磷素的吸收。植物细胞膜H⁺-ATPase在矿质营养元素吸收过程中具有重要调控作用,因此不同氮素形态调控茶树磷素吸收可能与细胞膜H⁺-ATPase相关。本研究采用二相分离法提取茶树根系质膜,并通过非损伤微测（NMT）、Western-blot等技术探究不同氮素形态对舒茶早根系磷素吸收和细胞膜H⁺-ATPase特征参数的影响。结果表明,铵态氮提高茶树对磷素的吸收;其茶树根系细胞膜电位、H⁺跨膜运输、H⁺-ATPase活性和蛋白表达均高于硝态氮处理;且细胞膜H⁺-ATPase专一抑制剂正钒酸钠（Na₃VO₄）显著减少不同氮素形态下茶树根系对磷素的吸收和富集。由此可见,茶树根系H⁺-ATPase可能参与不同氮素形态调控磷素的吸收。

关键词: 铵态氮, 硝态氮, 磷素吸收, 细胞膜H⁺-ATPase, 茶树

Abstract: Phosphorus is one of the most important mineral elements for plant growth and development, and different nitrogen forms can regulate phosphorus uptake. As is well-known that plant plasma membrane (PM) H⁺-ATPase plays an important role in nutrition uptake, it may also relate to the different nitrogen-modulated phosphorus uptake. In our study, PM H⁺-ATPase in tea roots were isolated by two-phase partitioning in aqueous dextranT-500 and polyethylene glycol, and the effect of different nitrogen forms on phosphorus uptake and the characteristic parameters of PM H⁺-ATPase in tea roots were examined by using non-invasive micro-test technique and Western-blot. It was found that ammonium significantly improved the phosphorus accumulation in tea plants as compared with nitrate nutrition. In addition, less depolarized PM potentials, higher net H⁺ flux, PM H⁺-ATPase activities and protein levels were found under ammonium treatment than those under nitrate nutrition treatment. Furthermore, vanadate (the PM H⁺-ATPase inhibitor) significantly decreased phosphorus accumulation in tea plants under ammonium and nitrate nutrition treatments, which further suggests that PM H⁺-ATPase may be involved in phosphorus accumulation in tea plants regulated by different nitrogen forms.

Key words: ammoniacal nitrogen, nitrate nitrogen, phosphorus absorption, PM H⁺-ATPase, tea plants

中图分类号:

陈林木, 陈景光, 王宁宁, 张显晨. 质子泵在不同氮素形态调控茶树磷素吸收的功能研究[J]. 茶叶科学, 2019, 39(6): 723-730. doi: 10.13305/j.cnki.jts.2019.06.011.

CHEN Linmu, CHEN Jingguang, WANG Ningning, ZHANG Xianchen. The Role of Plasma Membrane H⁺-ATPase on Nitrogen-regulated Phosphorus Uptake in Tea Plants[J]. Journal of Tea Science, 2019, 39(6): 723-730. doi: 10.13305/j.cnki.jts.2019.06.011.

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