茶树对硒吸收累积特性及其硒调控相关基因的表达分析

doi:10.13305/j.cnki.jts.20191202.002

摘要/Abstract

摘要： 本文采用沙培试验,从动态角度研究了不同时间和不同浓度下茶树对硒的吸收累积规律,并分析相关基因的表达特点。结果发现,茶树不同部位对硒的累积量存在较大差异,大部分硒保留在根部,在其体内的迁移率较低,硒的累积量和外源硒浓度、培养时间显著相关;当外源硒浓度在0~0.05 mmol·L^-1时,茶树长势良好,但当浓度高于0.10 mmol·L^-1时,茶树表现出中毒症状。荧光定量PCR分析发现,转录因子CsbHLH62及茉莉酸信号途径中的关键基因丙二烯环化氧化酶基因（CsAOC）和脂氧合酶基因（CsLOX6）受到高浓度硒的诱导表达,相关性分析表明,这3个基因的表达量与根系硒含量呈显著正相关。本研究表明,茶树各部位对硒的累积与外源硒浓度和培养时间显著相关,基因CsbHLH62、CsAOC和CsLOX6可能在该过程中发挥着重要作用。

关键词: 茶树, 硒, 吸收累积, 基因表达

Abstract: The concentration dependence and time course of selenium (Se) absorption and accumulation in tea plant were investigated under sand culture and the expressions of related genes were also analyzed. The results show that the absorption amounts of Se in different tissues of tea plant were remarkably different. Most of them were fixed by the roots, and low movement from roots to shoots was observed. Moreover, the accumulation of Se was significantly correlated with exogenous Se concentration and culture duration. Furthermore, tea plant grew well in the Se concentration from 0 to 0.05 mmol·L^-1, but when the concentration was higher than 0.10 mmol·L^-1, tea plant showed poisoning symptoms. Fluorescence quantitative PCR analysis indicates that allene oxide cyclase (CsAOC), lipoxygenase (CsLOX6) (key genes of jasmonic acid signaling pathway), as well as a basic helix-loop-helix transcription factor (CsbHLH62) could be obviously induced by high concentration of Se. The correlation analysis showes that the expressions of these genes were positively related with the Se content in roots. These results suggest that Se accumulation in different tissues of tea plant was significantly correlated with exogenous Se concentration and culture duration, and CsbHLH62, CsAOC, CsLOX6 might play important roles in this process.

Key words: tea plant, Se, absorption and accumulation, gene expression

中图分类号:

S571.1
Q52

曹丹, 马林龙, 刘艳丽, 龚自明, 金孝芳. 茶树对硒吸收累积特性及其硒调控相关基因的表达分析[J]. 茶叶科学, 2020, 40(1): 77-84. doi: 10.13305/j.cnki.jts.20191202.002.

CAO Dan, MA Linlong, LIU Yanli, GONG Ziming, JIN Xiaofang. Absorption and Accumulation Characteristics of Selenium in Tea Plant (Camellia sinensis) and Expression Analysis of Genes Related to Selenium Regulation[J]. Journal of Tea Science, 2020, 40(1): 77-84. doi: 10.13305/j.cnki.jts.20191202.002.

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