基于高通量测序的贮藏白牡丹表面细菌多样性分析

doi:10.13305/j.cnki.jts.2020.04.008

茶叶科学 ›› 2020, Vol. 40 ›› Issue (4): 519-527.doi: 10.13305/j.cnki.jts.2020.04.008

基于高通量测序的贮藏白牡丹表面细菌多样性分析

陈佳佳^1,2,4, 胡雲飞^2,3,4, 沈诗钰^1,2,4, 王志华^1,2,4, 周喆^1,2,4, 唐琴^1,2,4, 孙威江^1,2,3,4,*

1.福建农林大学园艺学院,福建福州 352000;
2.福建省行业技术开发基地,福建福州 350002;
3.福建农林大学安溪茶学院,福建泉州 362400;
4.福建省茶产业工程技术研究中心,福建福州 350002

收稿日期:2019-12-23 修回日期:2020-03-02 出版日期:2020-08-15 发布日期:2020-08-18
通讯作者: *swj8013@126.com
作者简介:陈佳佳,女,硕士研究生,主要从事茶叶加工方面的研究,1160576736@qq.com。
基金资助:
国家重点研发计划项目（2016YFF0201904-03）、国家自然科学基金项目（31770732）、福建农林大学茶产业链科技创新与服务体系建设

Analysis of Bacterial Diversity of Stored White Peony by High-Throughput Sequencing

CHEN Jiajia^1,2,4, HU Yunfei^2,3,4, SHEN Shiyu^1,2,4, WANG Zhihua^1,2,4, ZHOU Zhe^1,2,4, TANG Qin^1,2,4, SUN Weijiang^1,2,3,4,*

1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 352000, China;
2. Industrial Technology Development Base in Fujian Province, Fuzhou 350002, China;
3. Anxi College of Tea Science, Fujian Agriculture and Forestry University, Quanzhou 362400, China;
4. Tea Industry Engineering Technology Research Center of Fujian Province, Fuzhou 350002, China

Received:2019-12-23 Revised:2020-03-02 Online:2020-08-15 Published:2020-08-18

摘要/Abstract

摘要： 为分析贮藏白牡丹细菌多样性,利用高通量测序对11个不同贮藏年份白牡丹细菌进行16 S rDNA检测。结果表明,贮藏茶样的优势菌门为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、厚壁菌门（Firmicutes）等,优势菌纲为α-变形菌纲（Alphaproteobacteria）、γ-变形菌纲（Gammaproteobacteria）、鞘脂杆菌纲（Sphingobacteriia）等,优势菌属为鞘氨醇单胞菌属（Sphingomonas）、甲基杆菌属（Methylobacterium）、土地杆菌属（Pedobacter）等。不同贮藏年份白牡丹细菌组成结构相似,丰度存在差异;贮藏年份对白牡丹表面细菌组成未有显著影响,而贮藏环境影响细菌多样性。

关键词: 高通量测序, 白牡丹, 细菌, 多样性

Abstract: To investigate the diversity of the bacterial communities in stored white peony, 16 S rDNA of bacteria collected from 11 samples of different storage durations were analyzed by high-throughput sequencing. The results show that the species richness and diversity of DB13 were the highest. While in all stored white peony, the dominant phylum were Proteobacteria, Bacteroidetes and Firmicutes. The dominant class were Alphaproteobacteria, Gamma proteobacteria and Sphingobacteriia. And the dominant bacterial species at genus level include: Sphingomonas, Methylobacterium and Pedobacter. The composition structures of samples were similar, but there was difference in their abundance. Storage duration had no significant effect on the bacteria composition of white peony, but environments played important roles.

Key words: high-throughput sequencing, white peony, bacteria, diversity

中图分类号:

S571.1

陈佳佳, 胡雲飞, 沈诗钰, 王志华, 周喆, 唐琴, 孙威江. 基于高通量测序的贮藏白牡丹表面细菌多样性分析[J]. 茶叶科学, 2020, 40(4): 519-527. doi: 10.13305/j.cnki.jts.2020.04.008.

CHEN Jiajia, HU Yunfei, SHEN Shiyu, WANG Zhihua, ZHOU Zhe, TANG Qin, SUN Weijiang. Analysis of Bacterial Diversity of Stored White Peony by High-Throughput Sequencing[J]. Journal of Tea Science, 2020, 40(4): 519-527. doi: 10.13305/j.cnki.jts.2020.04.008.

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基于高通量测序的贮藏白牡丹表面细菌多样性分析

Analysis of Bacterial Diversity of Stored White Peony by High-Throughput Sequencing

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