小贯小绿叶蝉取食诱导抗、感茶树品种挥发物的释放

doi:10.13305/j.cnki.jts.2020.06.008

茶叶科学 ›› 2020, Vol. 40 ›› Issue (6): 795-806.doi: 10.13305/j.cnki.jts.2020.06.008

小贯小绿叶蝉取食诱导抗、感茶树品种挥发物的释放

任倩倩¹, 庄明珠¹, 蔡晓明², 边磊², 罗宗秀², 李兆群², 尤民生³, 陈宗懋^2,*, 金珊^1,*

1.茶学福建省高等学校重点实验室,福建农林大学园艺学院,福建福州 350002;
2.中国农业科学院茶叶研究所,浙江杭州 310008;
3.应用生态研究所,福建农林大学植物保护学院,福建福州 350002

收稿日期:2020-06-22 修回日期:2020-08-26 出版日期:2020-12-15 发布日期:2020-12-10
通讯作者: *zmchen2006@163.com;jinshan0313@163.com
作者简介:任倩倩,女,硕士研究生,主要从事茶叶质量安全研究。
基金资助:
闽台作物有害生物生态防控国家重点实验室开放课题（SKL20190010）、国家重点研发计划项目（2016YFE0102100）、国家茶叶质量安全工程技术研究中心开放课题（KIG17004A）

The Release of Volatiles in Resistant and Susceptible Tea Cultivars under Empoasca Onukii Feeding

REN Qianqian¹, ZHUANG Mingzhu¹, CAI Xiaoming², BIAN Lei², LUO Zongxiu², LI Zhaoqun², YOU Minsheng³, CHEN Zongmao^2,*, JIN Shan^1,*

1. College of Horticulture, Key Laboratory of Tea Science, Fujian Agricultural and Forest university, Fuzhou 350002, China;
2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
3. Institute of Applied Ecology, Agricultural and Forest university, Fuzhou 350002, China

Received:2020-06-22 Revised:2020-08-26 Online:2020-12-15 Published:2020-12-10

摘要/Abstract

摘要： 为研究不同抗性水平茶树应对小贯小绿叶蝉取食诱导的挥发物释放及相关代谢机制,以抗虫茶树品种举岩（JY）和感虫茶树品种恩标（EB）为试验材料,利用动态顶空收集法结合GC-MS技术检测茶树在小贯小绿叶蝉取食不同时间（6、12、24、36、48、72βh）释放的主要挥发物组分,并结合转录组数据分析主要挥发物合成途径上的相关基因的表达水平及其调控趋势。结果表明,在健康状态下,茶树释放的挥发性物质较少;在虫害后不同时间段的茶样中检测到顺-β-罗勒烯（β-Ocimene）、(E)-4,8-二甲基-1,3,7-壬三烯[(E)-4,8-dimethyl-1,3,7-nonatriene,DMNT]、芳樟醇（Linalool）、法尼烯（Farnesene）等10种主要挥发物。其中,单萜类物质在虫害诱导的感虫品种茶树上含量高,倍半萜类物质在虫害诱导的抗虫品种茶树中含量高。转录组数据显示,小贯小绿叶蝉取食诱导抗、感茶树品种中调控萜类合成的关键基因均被明显激活。调控单萜类物质合成的相关基因在感虫茶树品种中的表达量相对较高,而调控倍半萜类物质合成的相关基因在抗虫品种中的表达量与感虫品种差异不显著。本研究结果为茶树抗虫机制和小贯小绿叶蝉绿色防控提供一定的理论依据。

关键词: 茶树, 挥发物, 小贯小绿叶蝉, 萜类物质, 基因调控

Abstract: In order to study the volatile release and the relative metabolism induced by small green leafhoppers (Empoasca onukii) feeding in tea plants, the resistant tea cultivar (JY) and susceptible tea cultivar (EB) were used as materials. The time course (6, 12, 24, 36, 48, 72βh) of tea volatile compounds released by tea plants under E. onukii feeding were collected and detected by using dynamic headspace collection method and GC-MS technology. An integrative analysis of the metabolism and transcriptome data was then performed, and the target genes in the synthetic pathway of the main volatiles were screened and analyzed. The results show that healthy tea plants released less volatiles. While ten main volatiles such as β-Ocimene, DMNT, Linalool, Farnesene, etc. were identified in tea plants under E. onukii feeding. Among them, the contents of monoterpenoids were higher in the susceptible tea cultivar EB, and sesquiterpenoids were higher in the resistant tea cultivar JY. Furthermore, transcriptome data show that the key genes in terpene synthesis pathway were up-regulated in both cultivars after E. onukii feeding. The expression levels of genes related to the synthesis of monoterpenoids were relatively higher in susceptible tea cultivar than those in resistant cultivar. However, no significant expression differences were identified in genes related to sesquiterpenes synthesis in resistant and susceptible tea cultivars. The results of this study provided a theoretical basis for the tea resistance mechanism and green control of leafhopper in tea plantation.

Key words: tea plant, volatiles, Empoasca onukii, terpenoids, gene regulation

中图分类号:

任倩倩, 庄明珠, 蔡晓明, 边磊, 罗宗秀, 李兆群, 尤民生, 陈宗懋, 金珊. 小贯小绿叶蝉取食诱导抗、感茶树品种挥发物的释放[J]. 茶叶科学, 2020, 40(6): 795-806. doi: 10.13305/j.cnki.jts.2020.06.008.

REN Qianqian, ZHUANG Mingzhu, CAI Xiaoming, BIAN Lei, LUO Zongxiu, LI Zhaoqun, YOU Minsheng, CHEN Zongmao, JIN Shan. The Release of Volatiles in Resistant and Susceptible Tea Cultivars under Empoasca Onukii Feeding[J]. Journal of Tea Science, 2020, 40(6): 795-806. doi: 10.13305/j.cnki.jts.2020.06.008.

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小贯小绿叶蝉取食诱导抗、感茶树品种挥发物的释放

The Release of Volatiles in Resistant and Susceptible Tea Cultivars under Empoasca Onukii Feeding

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