一株对茶丽纹象甲高毒力白僵菌菌株的筛选、鉴定与培养研究

doi:10.13305/j.cnki.jts.2021.01.009

摘要/Abstract

摘要： 茶丽纹象甲（Myllocerinus aurolineatus）是一种重要的茶树害虫,当前对其防治仍以化学手段为主。为寻找一种高效、安全和可持续的生物防治方法,本研究采用浸虫法从12株原寄主为鞘翅目昆虫的白僵菌Beauveria spp.中筛选出一株对茶丽纹象甲成虫高毒力的Bbr1552菌株。室内生物测定结果表明,用每毫升含5.0×10⁷个孢子的孢悬液（25℃）处理茶丽纹象甲成虫的LT₅₀值为4.49 d,处理7 d,茶丽纹象甲的累计校正死亡率为100%,僵虫率为69.44%,LC₅₀值为每毫升1.55×10⁶个孢子。对菌株Bbr1552进行形态学鉴定与rDNA-ITS序列和Bloc序列分析,明确其为布氏白僵菌Beauveria brongniartii。此外,对该菌株的最适培养基和培养温度研究发现,其最适生长和产孢培养基均为PPDA培养基;最适生长和产孢温度均为25℃。

关键词: 茶丽纹象甲, 布氏白僵菌, 毒力, 鉴定, 培养条件

Abstract: Myllocerinus aurolineatus (Coleoptera: Curculionidae) is one of the most important pests in tea plantation, and chemical control is the usual treatment method for this weevil at present. In order to explore alternative strategy for sustainable control of the weevil, dip method was employed to test the virulence of 12 isolates of Beauveria sp., which isolated from insects of Coleoptera. When inoculated with spore suspension (5.0×10⁷ spores·mL^-1), the strain Bbr1552 showed the highest virulence against the adults of M. aurolineatus, which caused the highest corrected mortality rates of 100%, shortest LT₅₀ of 4.49 d and highest cadaver rates of 69.44%. The LC₅₀ value was 1.55×10⁶ spores·mL^-1 on 7 d (25℃) after inoculation with Bbr1552. According to morphological identification and analysis of rDNA-ITS and Bloc sequences, the strain Bbr1552 was identified to be a B. brongniartii. Besides, the PPDA medium and 25℃ were the most optimal conditions for the colony growth and sporulation of the strain Bbr1552.

Key words: Myllocerinus aurolineatus, Beauveria brongniartii, virulence, identification, cultural condition

中图分类号:

王定锋, 李良德, 李慧玲, 张辉, 吴光远. 一株对茶丽纹象甲高毒力白僵菌菌株的筛选、鉴定与培养研究[J]. 茶叶科学, 2021, 41(1): 101-112. doi: 10.13305/j.cnki.jts.2021.01.009.

WANG Dingfeng, LI Liangde, LI Huiling, ZHANG Hui, WU Guangyuan. Screening, Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus[J]. Journal of Tea Science, 2021, 41(1): 101-112. doi: 10.13305/j.cnki.jts.2021.01.009.

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