茶树中葡萄糖-6-磷酸脱氢酶基因（CsG6PDHs）的克隆与表达分析

doi:10.13305/j.cnki.jts.20210622.002

茶叶科学 ›› 2021, Vol. 41 ›› Issue (4): 497-510.doi: 10.13305/j.cnki.jts.20210622.002

茶树中葡萄糖-6-磷酸脱氢酶基因（CsG6PDHs）的克隆与表达分析

王彦丁¹, 王缓¹, 李娜娜², 王璐², 郝心愿², 王玉春³, 丁长庆², 杨亚军², 王新超^2,*, 钱文俊^1,*

1.青岛农业大学,山东青岛 266109;
2.中国农业科学院茶叶研究所/国家茶树改良中心/农业部茶树生物学与资源利用重点实验室,浙江杭州 310008;
3.浙江农林大学,浙江杭州 311300

收稿日期:2020-08-10 修回日期:2020-09-23 出版日期:2021-08-15 发布日期:2021-08-12
通讯作者: *xcw575@tricaas.com;qau-WenjunQian@qau.edu.cn
作者简介:王彦丁,女,本科,主要从事茶树遗传育种研究。
基金资助:
国家自然科学基金（31800588）、青岛农业大学科研启动基金（1118025）、青岛农业大学大学生创新创业训练项目

Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis

WANG Yanding¹, WANG Huan¹, LI Nana², WANG Lu², HAO Xinyuan², WANG Yuchun³, DING Changqing², YANG Yajun², WANG Xinchao^2,*, QIAN Wenjun^1,*

1. College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China;
2. Tea Research Institute of the Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China;
3. College of Agriculture and Food Science, Zhejiang A&F University, Hangzhou 311300, China;

Received:2020-08-10 Revised:2020-09-23 Online:2021-08-15 Published:2021-08-12

摘要/Abstract

摘要： 葡萄糖-6-磷酸脱氢酶（Glucose-6-phosphate dehydrogenase,G6PDH,EC1.1.1.49）是戊糖磷酸途径中的关键限速酶,在植物逆境胁迫响应和生长发育中具有重要作用。然而,目前有关G6PDH在茶树中的研究尚处空白。在茶树中克隆到3个CsG6PDHs基因,分别命名为CsG6PDH1（MW025829）、CsG6PDH2（MW025830）、CsG6PDH4（MW025831）。聚合进化树结果显示,CsG6PDH1和CsG6PDH4均为质体型蛋白,而CsG6PDH2为胞质型蛋白。表达分析发现,CsG6PDHs在不同组织中均有表达;低温或炭疽菌侵染条件下,CsG6PDH1和CsG6PDH4均被抑制表达;冷驯化期间,CsG6PDH2和CsG6PDH4在不同品种中均上调表达;此外,CsG6PDHs在茶芽休眠和萌发过程中均不同程度上调表达。以上结果表明,CsG6PDHs在茶树生长发育和逆境胁迫响应过程中发挥着重要作用,这为后续深入研究G6PDH在茶树中的功能奠定了理论基础。

关键词: 葡萄糖-6-磷酸脱氢酶, 茶树, 表达分析, 低温, 冷驯化

Abstract: As a key rate-limiting enzyme in pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PDH, EC1.1.1.49) plays an important role in plant growth and development, and also in responding to environmental stresses. However, the function of G6PDH in tea plants has not yet been reported. In our study, 3 G6PDH genes were cloned from tea plant, named as CsG6PDH1 (MW025829), CsG6PDH2 (MW025830) and CsG6PDH4 (MW025831), respectively. Phylogenetic analysis shows that CsG6PDH1 and CsG6PDH4 belong to plastid protein, while CsG6PDH2 belongs to cytoplasmic protein. Quantitative analysis shows that the expressions of CsG6PDHs varied in different tissues. Under cold or Colletotrichum infection treatments, the time course expressions of both CsG6PDH1 and CsG6PDH4 were gradually reduced. Besides, both CsG6PDH1 and CsG6PDH4 were induced by cold acclimation in different tea cultivars. In addition, the expressions of CsG6PDHs were up-regulated during bud dormancy and flush periods. Consequently, our results implicate that CsG6PDHs are widely participated in tea plant growth and development, and also involved in responding to abiotic and biotic stresses. This study provided a theoretical basis for in-depth study of the function of CsG6PDHs in tea plants.

Key words: glucose-6-phosphate dehydrogenase, Camellia sinensis, expression analysis, cold, cold acclimation

中图分类号:

王彦丁, 王缓, 李娜娜, 王璐, 郝心愿, 王玉春, 丁长庆, 杨亚军, 王新超, 钱文俊. 茶树中葡萄糖-6-磷酸脱氢酶基因（CsG6PDHs）的克隆与表达分析[J]. 茶叶科学, 2021, 41(4): 497-510. doi: 10.13305/j.cnki.jts.20210622.002.

WANG Yanding, WANG Huan, LI Nana, WANG Lu, HAO Xinyuan, WANG Yuchun, DING Changqing, YANG Yajun, WANG Xinchao, QIAN Wenjun. Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis[J]. Journal of Tea Science, 2021, 41(4): 497-510. doi: 10.13305/j.cnki.jts.20210622.002.

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茶树中葡萄糖-6-磷酸脱氢酶基因（CsG6PDHs）的克隆与表达分析

Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis

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