L-茶氨酸对CUMS抑郁大鼠海马和肠道损伤的干预作用研究

doi:10.13305/j.cnki.jts.2021.04.005

茶叶科学 ›› 2021, Vol. 41 ›› Issue (4): 511-524.doi: 10.13305/j.cnki.jts.2021.04.005

L-茶氨酸对CUMS抑郁大鼠海马和肠道损伤的干预作用研究

陈美艳^1,2, 刘芬^1,2, 林勇^1,2,3,*, 左高隆^1,2, 左颖鹏^1,2, 刘仲华^1,2,3

1.湖南农业大学茶学教育部重点实验室,湖南长沙 410128;
2.国家植物功能成分利用工程技术研究中心,湖南长沙 410128;
3.湖南省植物功能成分利用协同创新中心,湖南长沙 410128

收稿日期:2020-11-02 修回日期:2021-03-13 出版日期:2021-08-15 发布日期:2021-08-12
通讯作者: *ly2005306@163.com
作者简介:陈美艳,女,硕士研究生,主要从事茶叶功能成分利用相关研究。
基金资助:
湖南省自然科学基金项目（2020JJ4351、2017JJ3106）、湖南省教育厅科学研究重点项目（19A223）、湖南省科技特派员创新创业项目（2020NK4260）

Intervention Effects of L-Theanine on the Damage of Hippocampus and Gut in CUMS Depressed Rats

CHEN Meiyan^1,2, LIU Fen^1,2, LIN Yong^1,2,3,*, ZUO Gaolong^1,2, ZUO Yingpeng^1,2, LIU Zhonghua^1,2,3

1. Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
3. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, China

Received:2020-11-02 Revised:2021-03-13 Online:2021-08-15 Published:2021-08-12

摘要/Abstract

摘要： 以SPF级SD雄性大鼠为实验动物,建立慢性不可预见轻度应激（Chronic unpredictable mild stress,CUMS）大鼠模型,给予不同剂量L-茶氨酸灌胃干预,观察L-茶氨酸对CUMS模型大鼠的抑郁样行为、海马和肠道组织病理变化,以及白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）、5-羟色胺（5-HT）和胰高血糖素样肽1（GLP-1）含量的影响,探讨L-茶氨酸干预对CUMS抑郁大鼠海马和肠道损伤及功能异常的作用。结果表明,相比模型组,L-茶氨酸各剂量组大鼠体质量、糖水偏好、横穿格数和站立次数、水中的活跃度、进入明箱次数及停留时间均有不同程度的改善,中剂量组（100 mg·kg^-1）达到显著水平;L-茶氨酸有效修复了CUMS造成的海马神经元损伤,降低了CUMS大鼠血清、结肠和盲肠中的IL-6和TNF-α含量,并减轻结肠、盲肠组织的损伤和炎症程度,且以中剂量组效果较好;L-茶氨酸能增加CUMS大鼠海马、大脑皮层以及结肠中5-HT、GLP-1的含量,部分条件下达到显著水平。由此可见,L-茶氨酸具有改善CUMS模型大鼠抑郁样行为、修复海马和肠道结构损伤以及功能异常等作用,其对“肠-脑轴”的调节可能是抗抑郁的作用机制。

关键词: L-茶氨酸, 抑郁样行为, 海马, 肠道损伤, 肠-脑轴

Abstract: Using SPF-grade SD male rats as experimental animals, a rat model of chronic unpredictable mild stress (CUMS) was established and different doses of L-theanine were given intragastrically for intervention. Then, the effects of L-theanine on the depression-like behaviors, histopathological changes and the levels of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), 5-hydroxytryptamine (5-HT) and glucagon-like peptide 1 (GLP-1) contents of hippocampus and gut were observed and evaluated, which aimed to explore the intervention effects of L-theanine on the structural damage and dysfunction of hippocampus and gut in CUMS depressed rats. The results show that, compared with the model group, pretreatment with different doses of L-theanine improved the body weight,sugar-water preference, number of crossing and rearing, activity in water, number of entering the light box and the duration time of ratsat different degrees, especially the middle-dose group (100 mg·kg^-1) reached a significant level. L-theanine effectively repaired hippocampal neuron damage caused by CUMS, reduced IL-6 and TNF-α levels in serum, colon and cecum and attenuated the damage and inflammation degree of the colon and cecum tissues, and the middle-dose group also exhibited better effect. L-theanine could increase the contents of 5-HT and GLP-1 in hippocampus, cerebral cortex and colon of CUMS rats, and reached significant levels under some conditions. These results indicate that L-theanine can effectively improve the depression-like behavior, structural damage and dysfunction of hippocampus and gut in CUMS model rats, and its regulation of ‘gut-brain axis’ may be the action mechanism of anti-depression.

Key words: L-theanine, depression-like behavior, hippocampus, gut damage, gut-brain axis

中图分类号:

S571
R749.4

陈美艳, 刘芬, 林勇, 左高隆, 左颖鹏, 刘仲华. L-茶氨酸对CUMS抑郁大鼠海马和肠道损伤的干预作用研究[J]. 茶叶科学, 2021, 41(4): 511-524. doi: 10.13305/j.cnki.jts.2021.04.005.

CHEN Meiyan, LIU Fen, LIN Yong, ZUO Gaolong, ZUO Yingpeng, LIU Zhonghua. Intervention Effects of L-Theanine on the Damage of Hippocampus and Gut in CUMS Depressed Rats[J]. Journal of Tea Science, 2021, 41(4): 511-524. doi: 10.13305/j.cnki.jts.2021.04.005.

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L-茶氨酸对CUMS抑郁大鼠海马和肠道损伤的干预作用研究

Intervention Effects of L-Theanine on the Damage of Hippocampus and Gut in CUMS Depressed Rats

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