两个EoNPV毒株对茶尺蠖和灰茶尺蠖的毒力差异

doi:10.13305/j.cnki.jts.2021.04.008

摘要/Abstract

摘要： 通过确定不同茶尺蠖核型多角体病毒（Ectropis obliqua nucleopolyhedrovirus,EoNPV）毒株对茶尺蠖（Ectropis obliqua）和灰茶尺蠖（Ectropis grisescens）毒力水平的差异,为有效提高茶尺蠖病毒的防效提供理论基础。采用浸渍法,测定EoNPV浙江毒株（EoNPV-ZJ）和江西毒株（EoNPV-JX）对茶尺蠖和灰茶尺蠖3龄幼虫的毒力水平;通过克隆测序,多重比较分析EoNPV-ZJ和EoNPV-JX毒株同源重复区（hrs）。结果表明,EoNPV-JX对灰茶尺蠖和茶尺蠖3龄幼虫14 d的LC₅₀分别为5.95×10⁶ PIB·mL^-1和3.14×10⁶ PIB·mL^-1,EoNPV-ZJ对灰茶尺蠖和茶尺蠖3龄幼虫14 d的LC₅₀分别为1.13×10⁷ PIB·mL^-1和5.04×10⁶ PIB·mL^-1。EoNPV-JX和EoNPV-ZJ的hr1大小均为1 795 bp,含有11个完全回文序列,hr3大小均为665 bp,含有3个完全回文序列,与已报道的安徽毒株（EoNPV-AH）无差异;而hr2差异较大,其中EoNPV-JX hr2为864 bp,含有7个完全回文序列,EoNPV-ZJ hr2为1 168 bp,含有12个完全回文序列,均少于EoNPV-AH的18个完全回文序列。综合分析显示,EoNPV不同毒株对茶尺蠖的毒力水平高于其近缘种灰茶尺蠖;EoNPV-JX毒株对灰茶尺蠖的毒力高于EoNPV-ZJ毒株,造成EoNPV不同毒株毒力差异的主要原因可能与其hr2序列回文序列个数相关。

关键词: 茶尺蠖核型多角体病毒, 毒株, 茶尺蠖, 灰茶尺蠖, 毒力, 同源重复区

Abstract: The virulence differences of Ectropis obliqua nucleopolyhedrovirus (EoNPV) from different isolates to Ectropis obliqua and Ectropis grisescens were analyzed, which provided a basis for effectively improving the control effect of EoNPV. The virulence of EoNPV Zhejiang strain (EoNPV-ZJ) and EoNPV Jiangxi strain (EoNPV-JX) to third instar larvae of E. obliqua and E. grisescens were determined by leaf dipping. The homologous regions (hrs) of EoNPV-ZJ and EoNPV-JX were further sequenced and analyzed by multiple sequence alignment. The results indicate that the 14 d LC₅₀ of EoNPV-JX to E. grisescens and E. obliqua were 5.95×10⁶ PIB·mL^-1 and 3.14×10⁶ PIB·mL^-1, respectively, while the 14 d LC₅₀ of EoNPV-ZJ to the two tea geometrid moths were 1.13×10⁷ PIB·mL^-1 and 5.04×10⁶ PIB·mL^-1, respectively. The hr1 of EoNPV-JX and EoNPV-ZJ were both 1 795 bp in size, containing 11 complete palindrome sequences, and hr3 were both 665 bp in size, containing 3 complete palindrome sequences, which were the same as EoNPV Anhui strain (EoNPV-AH). However, the hr2 regions of the three strains were different. The hr2 of EoNPV-JX was 864 bp in size and contained 7 complete palindrome sequences, and it was 1 168 bp in size and contained 12 complete palindrome sequences in EoNPV-ZJ, all of which were less than the 18 complete palindrome sequences of hr2 in EoNPV-AH. Our study suggests that the virulence of different strains of EoNPV to E. obliqua were higher than that of its related species, E. grisescens, while the toxicity of EoNPV-JX to E. grisescens was more than that of EoNPV-ZJ. The sensitivity of E. obliqua to EoNPV was higher than that of E. grisescens. The virulence of EoNPV might be related to the number of palindromes of hr2.

Key words: EoNPV, strain, Ectropis obliqua, Ectropis grisescens, virulence, homologous regions

中图分类号:

徐彬, 韩光杰, 祁建杭, 李传明, 徐健, 陆玉荣, 刘琴. 两个EoNPV毒株对茶尺蠖和灰茶尺蠖的毒力差异[J]. 茶叶科学, 2021, 41(4): 545-552. doi: 10.13305/j.cnki.jts.2021.04.008.

XU Bin, HAN Guangjie, QI Jianhang, LI Chuanming, XU Jian, LU Yurong, LIU Qin. Virulence Difference of Two Strains of EoNPV Isolates to Ectropis obliqua and Ectropis grisescens[J]. Journal of Tea Science, 2021, 41(4): 545-552. doi: 10.13305/j.cnki.jts.2021.04.008.

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