欢迎访问《茶叶科学》,今天是

茶叶科学 ›› 2021, Vol. 41 ›› Issue (5): 669-680.doi: 10.13305/j.cnki.jts.20210914.001

• 研究报告 • 上一篇    下一篇

从肠道菌群改变探讨青砖茶对非酒精性脂肪肝的预防作用

周婷婷1, 陈桂婷1, 曹楠1, 何建刚2, 何功威2, 肖长义1,2, 李世刚1,2,*   

  1. 1.三峡大学医学院,湖北宜昌 443002;
    2.湖北长盛川青砖茶研究所,湖北宜昌 443000
  • 收稿日期:2021-02-05 修回日期:2021-04-23 出版日期:2021-10-15 发布日期:2021-10-12
  • 通讯作者: *fox201@163.com
  • 作者简介:周婷婷,女,硕士,主要从事中药有效性与安全性评价方面的研究。
  • 基金资助:
    湖北省区域创新发展科技专项(2020BGC015)、宜昌市科技研究与开发项目(A21-1-075)、三峡大学学位论文培优基金资助项目(2020SSPY112)

Preventive Effect of Green Brick Tea on Non-alcoholic Fatty Liver Disease via Gut Microbiota Changes

ZHOU Tingting1, CHEN Guiting1, CAO Nan1, HE Jiangang2, HE Gongwei2, XIAO Changyi1,2, LI Shigang1,2,*   

  1. 1. Medical College of China Three Gorges University, Yichang 443002, China;
    2. Changshengchuan Green-brick Tea Research Institute of Hubei Province, Yichang 443000, China
  • Received:2021-02-05 Revised:2021-04-23 Online:2021-10-15 Published:2021-10-12

摘要: 通过肠道菌群改变研究青砖茶(GBT)对小鼠非酒精性脂肪肝(NAFLD)的预防作用。C57BL/6小鼠随机分为5组,即正常对照组(NC),模型对照组(MC),阳性药物对照组(PC)以及青砖茶低剂量组(LD)、高剂量组(HD),高脂饲料喂养小鼠建立NAFLD模型,同时预防性给予低、高剂量青砖茶水提取物和阳性药物血脂康,分别测定小鼠的体重、食物利用率、肝重、肝指数、TC、LDL-C/HDL-C及ALT含量,HE染色和油红O染色观察肝组织病理切片,ELISA检测肝组织IL-1β、IL-18含量变化,16 S rDNA V3-V4区高通量测序分析肠道菌群变化,Spearman相关性分析方法分析菌群与NAFLD表型的相关性。与模型组比较,青砖茶组小鼠体重、食物利用率、肝重、肝指数、血清TC、LDL-C/HDL-C、ALT、肝组织TC、IL-1β、IL-18含量均有显著性降低,肝脏病变程度有所改善;肠道菌群分析及相关性分析显示Bacteroides物种丰度降低,且与NAFLD表型呈正相关,LactobacillusAlloprevotellaSaccharibacteria_genera_incertae_sedis物种丰度增加,且与NAFLD表型呈负相关,与NAFLD表型相关性最强的菌群为BacteroidesLactobacillus。青砖茶对NAFLD具有一定的预防作用,其作用可能与影响肠道菌群的改变有关。

关键词: 肠道菌群, 非酒精性脂肪肝, 青砖茶, 高通量测序, 相关性分析

Abstract: The preventive effect of green brick tea (GBT) on the mouse non-alcoholic fatty liver disease (NAFLD) model was studied by affecting changes in gut microbiota. C57BL/6 mice were randomly divided into 5 groups, including normal control group (NC), model control group (MC), positive drug control group (PC), low-dose group (LD) and high-dose group (HD) of GBT. A NAFLD model was established by feeding mice with high-fat diet, and supplemented with low and high doses of GBT water extract and positive drug (Xuezhikang) respectively. The body weight, food utilization efficiency, liver weight, liver index, TC, LDL-C/HDL-C and ALT contents of mice were determined. Liver tissue pathological sections were observed by HE staining and Oil Red O staining. ELISA method was used to detect changes of IL-1β and IL-18 in liver tissue. The changes of gut microbiota were analyzed by high-throughput sequencing in 16 S rDNA V3-V4 region, and Spearman's correlation analysis method was used to analyze the correlation between gut microbiota and NAFLD phenotype. Compared with the model control group, the body weight, food utilization efficiency, liver weight, liver index, serum TC, LDL-C/HDL-C, ALT, liver tissue TC, IL-1β, and IL-18 contents of mice in the GBT group were significantly reduced, and the degree of liver disease was improved. Gut microbiota analysis and correlation analysis show that the species abundance of Bacteroides decreased, and it was positively correlated with the NAFLD phenotype. The species abundance of Lactobacillus, Alloprevotella, and Saccharibacteria_genera_incertae_sedis increased, and they were negatively correlated with the NAFLD phenotype. Bacteroides and Lactobacillus had the strongest correlation with NAFLD phenotype. Green brick tea has a certain preventive effect on NAFLD, and its effect may be related to the changes in gut microbiota.

Key words: gut microbiota, NAFLD, green brick tea, high-throughput sequencing, correlation analysis

中图分类号: