重度遮阴及复光条件下茶树根系的生理响应及抗氧化酶活性动态变化

doi:10.13305/j.cnki.jts.2021.05.005

茶叶科学 ›› 2021, Vol. 41 ›› Issue (5): 695-704.doi: 10.13305/j.cnki.jts.2021.05.005

重度遮阴及复光条件下茶树根系的生理响应及抗氧化酶活性动态变化

焦海珍^1,2,3, 邵陈禹^1,2,3, 陈建姣^1,2,3, 张晨禹^1,2,3, 陈佳豪^1,2,3, 李云飞^1,2,3, 沈程文^1,2,3,*

1.湖南农业大学茶学教育部重点实验室,湖南长沙 410128;
2.湖南农业大学国家植物功能成分利用工程技术研究中心,湖南长沙 410128;
3.湖南农业大学园艺学院,湖南长沙 410128

收稿日期:2021-02-02 修回日期:2021-03-09 出版日期:2021-10-15 发布日期:2021-10-12
通讯作者: *scw69@163.com
作者简介:焦海珍,女,硕士研究生,主要从事茶树生物技术与种质创新研究,894622040@qq.com。
基金资助:
中央引导地方科技发展专项（2019XF5041）、湖南省现代农业产业技术体系建设专项（湘财农指[2020]112号）

Dynamic Changes of Physiological Responses and Antioxidant Enzyme Activities in Tea Root System under Severe Shading and Removal of Shading

JIAO Haizhen^1,2,3, SHAO Chenyu^1,2,3, CHEN Jianjiao^1,2,3, ZHANG Chenyu^1,2,3, CHEN Jiahao^1,2,3, LI Yunfei^1,2,3, SHEN Chengwen^1,2,3,*

1. Lab of Tea Science of China Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
2. National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, China;
3. College of Horticulture, Hunan Agricultural University, Changsha 410128, China

Received:2021-02-02 Revised:2021-03-09 Online:2021-10-15 Published:2021-10-12

摘要/Abstract

摘要： 遮阴是改善夏秋茶品质的重要措施之一,关于遮阴对茶树鲜叶的影响已有诸多研究,但对茶树根系影响的研究还鲜见报道。通过盆栽试验,探讨湘妃翠和金萱在95%遮阴处理0、4、12 d及复光4 d的生理指标和抗氧化酶活性的动态变化。结果表明,遮阴持续12 d,茶树新发吸收根与一级侧根数增加,复光后4 d下降,根系活力变化趋势与此一致;遮阴后湘妃翠可溶性糖含量上升,复光后4 d湘妃翠和金萱可溶性糖分别显著上升5.82%和8.04%。重度遮阴4 d,两个品种的抗坏血酸过氧化物酶(APX)和过氧化物酶（POD）活性均较高;遮阴12 d时,超氧化物歧化酶（SOD）、APX活性较高,而复光后APX活性显著高于未遮阴。以上研究说明,茶树根系通过增加可溶性糖的含量进行渗透调节,增强根系活力以吸收和运输养分,并通过提高抗氧化酶活性清除自由基来响应重度遮阴环境。遮光率为95%的遮阴环境下,金萱更耐阴,而复光可能对茶树的根系造成一定程度胁迫。

关键词: 茶树, 遮阴, 根系活力, 抗氧化酶, 根系生理

Abstract: Shading is one of the important measures to improve the quality of summer and autumn tea. There were a lot of studies on the effect of shading on tea leaves, but there were few reports on the root system. In this study, a pot experiment was used to explore the dynamic changes of physiological indexes and antioxidant enzyme activities of ‘Xiangfeicui’ and ‘Jinxuan’ under 95% shading treatment. Data were collected after shading for 0, 4, 12 d and the 4 d after regaining light. The results show that the number of new absorbing roots and lateral roots of tea plants increased after shading for 12 d, and decreased on the 4th day after regaining light. The trends of root vigor changes were the same. The soluble sugar content of ‘Xiangfeicui’ increased after shading, and the soluble sugars of ‘Xiangfeicui’ and ‘Jinxuan’ increased by 5.82% and 8.04% on the 4th day after regaining light. On the 4th day of heavy shading, the APX and POD activities of the two cultivars reached the peaks. On the 12th day of shading, the activities of SOD and APX the highest. The APX activity of tea roots after regaining light was significantly higher than that without shading. This study reveals that the roots of tea plants were osmotically regulated by increasing the content of soluble sugars, enhancing root vigor to absorb and transport nutrients, and increasing antioxidant enzyme activities to scavenging free radicals in response to heavy shade environments. Under a shading environment (95%), ‘Jinxuan’ was more shade-tolerant, and regaining light might cause a certain degree of stress to the roots of tea plants.

Key words: Camellia sinensis, shading, root vigor, antioxidant enzyme, root physiology

中图分类号:

S571.1

焦海珍, 邵陈禹, 陈建姣, 张晨禹, 陈佳豪, 李云飞, 沈程文. 重度遮阴及复光条件下茶树根系的生理响应及抗氧化酶活性动态变化[J]. 茶叶科学, 2021, 41(5): 695-704. doi: 10.13305/j.cnki.jts.2021.05.005.

JIAO Haizhen, SHAO Chenyu, CHEN Jianjiao, ZHANG Chenyu, CHEN Jiahao, LI Yunfei, SHEN Chengwen. Dynamic Changes of Physiological Responses and Antioxidant Enzyme Activities in Tea Root System under Severe Shading and Removal of Shading[J]. Journal of Tea Science, 2021, 41(5): 695-704. doi: 10.13305/j.cnki.jts.2021.05.005.

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重度遮阴及复光条件下茶树根系的生理响应及抗氧化酶活性动态变化

Dynamic Changes of Physiological Responses and Antioxidant Enzyme Activities in Tea Root System under Severe Shading and Removal of Shading

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