L-茶氨酸改善来曲唑诱导的多囊卵巢综合征大鼠临床症状

茶叶科学 ›› 2021, Vol. 41 ›› Issue (6): 831-842.

L-茶氨酸改善来曲唑诱导的多囊卵巢综合征大鼠临床症状

黄秋萍¹, 谢晨阳¹, 李新宇¹, 金斌¹, 曾榛^1,2, 钱波^1,3, 戴玲^6,*, 宋家乐^1,4,5,7,*

1.桂林医学院公共卫生学院,广西桂林 541199;
2.中南大学湘雅公共卫生学院,湖南长沙 410000;
3.厦门大学公共卫生学院,福建厦门 361102;
4.广西卫生健康委员会全生命周期健康保健研究实验室,广西桂林 541199;
5.广西环境暴露组学与全生命周期健康重点实验室,广西桂林 541199;
6.桂林医学院大学生心理健康与咨询中心,广西桂林 541199;
7.桂林医学院第二附属医院临床营养科,广西桂林 541109

收稿日期:2021-03-25 修回日期:2021-06-22 出版日期:2021-12-15 发布日期:2021-12-09
通讯作者: * 305743680@qq.com;songjiale@glmc.edu.cn
作者简介:黄秋萍,女,本科,主要从事营养与食品卫生学方面的研究。
基金资助:
人社部“高层次留学人才回国资助计划”（人社厅函[2019]160号）、广西高等学校千名中青年骨干教师培育计划资助（桂教人[2018]18号）、广西高校中青年教师科研基础能力提升项目（2018KY0401）、桂林医学院引进人才科研启动基金（04010150001）、2020年国家级大学生创新创业训练计划项目（202010601031）

L-Theanine Ameliorated Clinical Symptoms in Letrozole-Induced Polycystic Ovary Syndrome Rats

HUANG Qiuping¹, XIE Chenyang¹, LI Xinyu¹, JIN Bin¹, ZENG Zhen^1,2, QIAN Bo^1,3, DAI Ling^6,*, SONG Jiale^1,4,5,7,*

1. School of Public Health, Guilin Medical University, Guilin 541199, China;
2. Xiangya School of Public Health, Central South University, Changsha 410000, Hunan;
3. School of Public Health, Xiamen University, Xiamen 361102, China;
4.Guangxi Health Commission Key Laboratory of Entire Lifecycle Health and Care, Guilin 541199, China;
5. Guangxi Key Laboratory of Enviromental Exposomics and Entire Lifecycle Health, Guilin 541199, China;
6. Center of Mental Health Education and Counseling, Guilin Medical University, Guilin 541199, China;
7. Department of Clinical Nutrition, The Second Affiliated Hospital of Guilin Medical College, Guilin 541109, China

Received:2021-03-25 Revised:2021-06-22 Online:2021-12-15 Published:2021-12-09

摘要/Abstract

摘要： 为探究L-茶氨酸（L-theanine,LTA）对来曲唑诱导的多囊卵巢综合征（Polycystic ovarian syndrome,PCOS）大鼠临床症状的改善效果。将28只雌性SD大鼠按每组7只随机分为正常对照组、PCOS模型组、PCOS+低剂量LTA组和PCOS+高剂量LTA组。建模阶段,除正常对照组以生理盐水灌胃外,其余3组持续灌胃来曲唑28 d诱导PCOS模型。干预阶段,正常对照组和PCOS模型组以生理盐水持续灌胃,两个LTA干预组大鼠分别持续灌胃对应剂量LTA干预30 d。结果显示,与PCOS模型组相比,LTA干预组大鼠血清中睾酮（Testosterone,T）、黄体生成素（Luteinizing hormone,LH）、胰岛素（Insulin,INS）、甘油三酯（Triglyceride,TG）、总胆固醇（Total cholesterol,TC）、低密度脂蛋白（Low-density lipoprotein cholesterol,LDL-C）、脂多糖（Lipopolysaccharide,LPS）水平及胰岛素抵抗指数（HOMA-IR）均显著降低（P<0.05）,高密度脂蛋白（High-density lipoprotein cholesterol,HDL-C）水平均显著升高（P<0.05）,黄体生成素与卵泡刺激素的比值（LH/FSH）、空腹血糖（Fasting plasma glucose,FPG）和体重均有所下降（P>0.05）;低剂量LTA干预组大鼠血清雌二醇（Estradiol,E2）显著升高（P<0.05）;高剂量LTA干预组大鼠卵泡刺激素（Follicle stimulating hormone,FSH）显著升高（P<0.05）;LTA干预组大鼠的发情周期紊乱、卵巢组织多囊样病变较PCOS模型组均有一定程度的改善。结果表明,LTA干预能有效调节PCOS大鼠性激素分泌、恢复发情周期规律变化和改善其卵巢多囊样病变,同时显著改善PCOS大鼠胰岛素抵抗（Insulin resistance,IR）、血脂代谢紊乱并抑制异常水平的脂多糖（Lipopolysaccharide,LPS）分泌。

关键词: L-茶氨酸, 多囊卵巢综合征, 高雄激素血症, 胰岛素抵抗

Abstract: To explore the improvement effect of L-theanine (LTA) on the clinical symptoms of letrozole-induced polycystic ovary syndrome (PCOS) rats, twenty-eight female SD rats were randomly divided into control group, PCOS group, PCOS+low-dose LTA group, and PCOS+high-dose LTA group with 7 rats in each group. In the modeling stage, except for the control group, the other three groups were administered letrozole continuously for 28 days to induce the PCOS model. During the intervention phase, the normal control group and the PCOS group were given continuous gastric administration with normal saline, and the rats in the two LTA groups were given continuous gastric administration with the corresponding dose of LTA intervention for 30 days. The results show that compared with the PCOS group, the serum testosterone (T), luteinizing hormone (LH), insulin (INS), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), lipopolysaccharide (LPS) levels and homeostasis model assessment-insulin resistance (HOMA-IR) index in the LTA intervention group were significantly reduced (P<0.05), high-density lipoprotein cholesterol (HDL-C) was significantly increased (P<0.05), ratio of luteinizing hormone to follicle stimulating hormone (LH/FSH), fasting plasma glucose (FPG) and body weight were decreased (P>0.05). The serum estradiol (E2) of rats in the low-dose LTA intervention group was significantly increased (P<0.05). Follicle stimulating hormone (FSH) of mice in the high-dose LTA intervention group was significantly increased (P<0.05). At the same time, rat estrous cycle disorder and polycystic lesions of ovarian tissue were improved to a certain extent compared with PCOS group. The results show that LTA intervention can effectively regulate the secretion of sex hormones in PCOS rats, restore the regular changes of the estrus cycle, improve their ovarian polycystic lesions, and significantly improve insulin resistance (IR) and blood lipid metabolism disorders in PCOS rats and inhibit abnormal levels of lipopolysaccharide (LPS) secretion.

Key words: L-theanine, polycystic ovary syndrome, hyperandrogenemia, insulin resistance

中图分类号:

S571.1
R373

黄秋萍, 谢晨阳, 李新宇, 金斌, 曾榛, 钱波, 戴玲, 宋家乐. L-茶氨酸改善来曲唑诱导的多囊卵巢综合征大鼠临床症状[J]. 茶叶科学, 2021, 41(6): 831-842.

HUANG Qiuping, XIE Chenyang, LI Xinyu, JIN Bin, ZENG Zhen, QIAN Bo, DAI Ling, SONG Jiale. L-Theanine Ameliorated Clinical Symptoms in Letrozole-Induced Polycystic Ovary Syndrome Rats[J]. Journal of Tea Science, 2021, 41(6): 831-842.

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