茯砖茶改善2型糖尿病小鼠代谢紊乱的效果研究

茶叶科学 ›› 2022, Vol. 42 ›› Issue (1): 63-75.

茯砖茶改善2型糖尿病小鼠代谢紊乱的效果研究

代昕玥^1,2, 葛炳钢^1,2, 张旭雯^1,2, 刘文武^1,4, 段继春⁵, 傅冬和^1,2,3,*

1.湖南农业大学茶学教育部重点实验室,湖南长沙 410128;
2.国家植物功能成分利用工程技术研究中心,湖南长沙 410128;
3.湖南省植物功能成分利用协同创新中心,湖南长沙 410128;
4.湖南省农业科学院茶叶研究所,湖南长沙 410125;
5.湖南德康茶业科技有限公司,湖南长沙 410001

收稿日期:2021-08-31 修回日期:2021-09-17 出版日期:2022-02-15 发布日期:2022-02-18
通讯作者: *40086713@qq.com
作者简介:代昕玥,女,硕士研究生,主要从事茶叶功能成分利用相关研究,974644744@qq.com。
基金资助:
国家重点研发计划（2018YFC1604403）、国家自然科学基金（32002095、32172217）

Effect of Fu Brick Tea on Improving Metabolic Disorders in Type 2 Diabetes Mice

DAI Xinyue^1,2, GE Binggang^1,2, ZHANG Xuwen^1,2, LIU Wenwu^1,4, DUAN Jichun⁵, FU Donghe^1,2,3,*

1. Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
3. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
4. Tea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China;
5. Hunan Dekang Tea Technology Co., Ltd, Changsha 410001, China

Received:2021-08-31 Revised:2021-09-17 Online:2022-02-15 Published:2022-02-18

摘要/Abstract

摘要： 为探究茯砖茶能否通过调节肠道微生物群改善2型糖尿病（T2DM）的代谢紊乱,建立T2DM小鼠模型,给予400 mg·kg^-1茯砖茶水提取物灌胃干预,观测T2DM小鼠的饮食量、饮水量、空腹体重、空腹血糖、糖耐量等日常指标,血清中胰岛素（INS）、总胆汁酸（TBA）、总胆固醇（TC）、总甘油三酯（TG）、低密度脂蛋白（LDL-C）、高密度脂蛋白（HDL-C）、白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）水平,以及胰腺、回肠的病理学变化,并对小鼠粪便进行了肠道菌群16 S rRNA测序。结果显示,茯砖茶显著改善了小鼠的糖脂代谢紊乱和炎症,明显修复了T2DM导致的胰腺和回肠损伤。茯砖茶能显著降低T2DM引起的疣微菌门相对丰度异常增加,恢复肠道微生物群多样性。茯砖茶能增加肠道厚壁菌门、Eubacterium_coprostanoligenes_group、肠杆菌属、Acetatifactor、双歧杆菌属、Prevotella_9、Faecalibacterium、A2、Coriobacteriaceae及Eggerthellaceae等有益菌,说明茯砖茶可通过增加肠道有益菌群,调节肠道菌群失调,进而改善T2DM的代谢紊乱。

关键词: 茯砖茶, 2型糖尿病, 肠道菌群

Abstract: To investigate whether Fu brick tea (FBT) water extract can attenuate metabolic disorders in Type 2 Diabetes (T2DM) by regulating gut microbiota, a T2DM mouse model was established with streptozocin and 400 mg·kg^-1 FBT water extract administration. Diet, and water consumption, body weight, fasting glucose and glucose tolerance in mice were observed. Serum levels of insulin (INS), total bile acid (TBA), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) were examined. Histopathological changes of ileum and pancreas were also observed. Furthermore, fecal samples were analyzed by 16 S rRNA gene sequencing. The results show that FBT reduced the serum lipid metabolism levels, blood glucose, and inflammatory cytokines. Simultaneously, FBT treatment significantly ameliorated pathological changes in the pancreas and ileum. Moreover, the diversity, structure and composition of T2DM-disrupted gut microbiota were restored by the supplementation of FBT. T2DM-induced increase in the relative abundance of Verrucomicrobia was remarkably restored by FBT. FBT increased the growth of many key beneficial bacteria, including Firmicutes, Bifidobacterium, Enterorhabdus, Prevotella_9, A2, Acetatifactor, Eubacterium_coprostanoligenes_group, Faecalibacterium, Coriobacteriaceae and Eggerthellaceae. Collectively, the study showed that FBT might alleviate dysbacteriosis and metabolic disorders in T2DM by increasing beneficial flora.

Key words: Fu brick tea, type 2 diabetes mellitus, gut microbiota

中图分类号:

代昕玥, 葛炳钢, 张旭雯, 刘文武, 段继春, 傅冬和. 茯砖茶改善2型糖尿病小鼠代谢紊乱的效果研究[J]. 茶叶科学, 2022, 42(1): 63-75.

DAI Xinyue, GE Binggang, ZHANG Xuwen, LIU Wenwu, DUAN Jichun, FU Donghe. Effect of Fu Brick Tea on Improving Metabolic Disorders in Type 2 Diabetes Mice[J]. Journal of Tea Science, 2022, 42(1): 63-75.

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