小檗碱对不同茶树炭疽菌的抑菌活性及作用机制

doi:10.13305/j.cnki.jts.2022.03.006

茶叶科学 ›› 2022, Vol. 42 ›› Issue (3): 367-375.doi: 10.13305/j.cnki.jts.2022.03.006

小檗碱对不同茶树炭疽菌的抑菌活性及作用机制

张志鹏, 程庆华, 谢靖康, 万宇鹤, 童华荣, 陈应娟^*

西南大学食品科学学院茶学系/食品科学与工程国家级实验教学示范中心,重庆 400715

收稿日期:2021-12-20 修回日期:2022-03-16 出版日期:2022-06-15 发布日期:2022-06-17
通讯作者: *cyj8@swu.edu.cn
作者简介:张志鹏,男,本科在读,主要从事茶树栽培与病虫防控相关研究,2072312650@qq.com。
基金资助:
重庆市技术创新与应用发展专项重点项目（cstc2021jscx-tpyzxX0010）、大学生创新创业训练项目（202110635062和S202110635293）、中央高校基本业务费重点项目（XDJK2020B046）

The Antifungal Effect and Mechanism of Berberine on Different Colletotrichum Species Causing Tea Brown Blight Disease

ZHANG Zhipeng, CHENG Qinghua, XIE Jingkang, WAN Yuhe, TONG Huarong, CHEN Yingjuan^*

National Demonstration Center for Experimental Food Science and Technology Education, Department of Tea Science, College of Food Science, Southwest University, Chongqing 400715, China

Received:2021-12-20 Revised:2022-03-16 Online:2022-06-15 Published:2022-06-17

摘要/Abstract

摘要： 为研究小檗碱在茶炭疽菌（Colletotrichum）引起的茶树叶部病害防治中的应用前景,以松针炭疽菌（C. fioriniae）、喀斯特炭疽菌（C. karstii）、重庆炭疽菌（C. chongqingense）、山茶炭疽菌（C. camelliae）和胶孢炭疽菌（C. gloeosporioides）5种不同茶炭疽菌为研究对象,研究了小檗碱对不同炭疽菌的抑菌活性,并比较了其抑菌活性差异。研究结果表明,小檗碱对C. camelliae和C. chongqinggense的抑制效果最好,在质量浓度为12 mg·mL^-1时达到100%抑菌率,其次是C. gloeosporioides、C. karstii和C. fioriniae,其抑菌中浓度（Concentration for 50% of maximal effect,EC₅₀）分别为2.828、3.288、4.164、4.778、5.104 mg·mL^-1。显微镜镜检发现,小檗碱对5种炭疽菌的菌丝和分生孢子均存在明显影响,随小檗碱浓度的增加,部分菌丝出现不规则膨大现象,分生孢子形态也出现扭曲变形;生物活性检测发现,小檗碱处理后,不同炭疽菌的细胞结构均出现不同程度损伤,细胞膜的通透性增大、细胞氧化应激反应增强。研究结果明确了小檗碱对茶树炭疽菌的抑菌活性及应用前景,为炭疽菌引起的茶树病害的防治提供了新的途径及理论依据。

关键词: 小檗碱, 茶炭疽菌, 抑菌活性, 作用机制

Abstract: To explore the application prospects of berberine on the prevention and control of tea diseases, the antifungal activity of berberine against five different Colletotrichum species (C. fioriniae, C. karstii, C. chongqinggense, C. camelliae and C. gloeosporioides) were studied and the differences in antifungal activities were compared. The results show that berberine has the best inhibitory effect on both C. camelliae and C. chongqinggense, reaching 100% at the concentration of 12 mg·mL^-1, followed by C. gloeosporioides, C. karstii and C. fioriniae. The EC₅₀ (Concentration for 50% of maximal effect) of the five Colletotrichum species were 2.828, 3.288, 4.164, 4.778 mg·mL^-1 and 5.104 mg·mL^-1, respectively. The conidial and hyphal morphology of five Colletotrichum species under the treatment of berberine were examined, showing that berberine had a significant impact on the mycelial and conidial growth of five Colletotrichum species. With the increase of berberine concentration, part of hyphae expanded irregularly and the conidia grew abnormally. Results of the biological activity of Colletotrichum species show that the cell structure was damaged, the permeability of cell membrane was increased and the cell oxidative stress response was enhanced. Results of this study firstly confirm the antifungal activity and application prospects of berberine against Colletotrichum species, which provided a new approach and theoretical basis for the prevention and control of tea disease caused by Colletotrichum.

Key words: berberine, Colletotrichum, antifungal activity, mechanism

中图分类号:

张志鹏, 程庆华, 谢靖康, 万宇鹤, 童华荣, 陈应娟. 小檗碱对不同茶树炭疽菌的抑菌活性及作用机制[J]. 茶叶科学, 2022, 42(3): 367-375. doi: 10.13305/j.cnki.jts.2022.03.006.

ZHANG Zhipeng, CHENG Qinghua, XIE Jingkang, WAN Yuhe, TONG Huarong, CHEN Yingjuan. The Antifungal Effect and Mechanism of Berberine on Different Colletotrichum Species Causing Tea Brown Blight Disease[J]. Journal of Tea Science, 2022, 42(3): 367-375. doi: 10.13305/j.cnki.jts.2022.03.006.

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小檗碱对不同茶树炭疽菌的抑菌活性及作用机制

The Antifungal Effect and Mechanism of Berberine on Different Colletotrichum Species Causing Tea Brown Blight Disease

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