茶树白化果皮超微结构观察及分子机制研究

doi:10.13305/j.cnki.jts.2022.06.009

茶叶科学 ›› 2022, Vol. 42 ›› Issue (6): 779-790.doi: 10.13305/j.cnki.jts.2022.06.009

茶树白化果皮超微结构观察及分子机制研究

汤榕津^1,2, 刘浩然^1,2, 刘丁丁^1,2, 张晨禹^1,2, 龚洋^1,2, 叶圆圆^1,2, 陈杰丹¹, 陈亮¹, 马春雷^1,*

1.中国农业科学院茶叶研究所/农业农村部特种经济动植物生物学与遗传育种重点实验室,浙江杭州 310008;
2.中国农业科学院研究生院,北京 100081

收稿日期:2022-09-29 修回日期:2022-11-10 出版日期:2022-12-15 发布日期:2023-01-04
通讯作者: * malei220@tricaas.com
作者简介:汤榕津,女,硕士研究生,主要从事茶树资源育种与遗传改良研究。
基金资助:
国家重点研发计划课题（2021YFD1200203）、浙江省农业（茶树）新品种选育重大科技专项子课题（2021C02067-6-1）、中国农业科学院茶叶研究所基本科研业务费（1610212022009）、浙江省自然科学基金（LQ20C160010）

The Ultrastructure and Molecular Mechanism of Albino Pericarp in Tea Plants

TANG Rongjin^1,2, LIU Haoran^1,2, LIU Dingding^1,2, ZHANG Chenyu^1,2, GONG Yang^1,2, YE Yuanyuan^1,2, CHEN Jiedan¹, CHEN Liang¹, MA Chunlei^1,*

1. Tea Research Institute of the Chinese Academy of Agricultural Science/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China;
2. Graduate School of Chinese Academy of Agriculture Science, Beijing 100081, China

Received:2022-09-29 Revised:2022-11-10 Online:2022-12-15 Published:2023-01-04

摘要/Abstract

摘要： 茶树白化果皮是茶树白化突变体中的稀有材料,目前还未有文献记载。以中茶129为对照,对云白1号白化果皮的超微结构进行观察,同时对两份材料的果皮、种子进行转录组分析。结果表明,与中茶129的绿色果皮相比,白化果皮细胞内的质体结构严重损坏,无法进一步发育形成叶绿体;富集分析表明,果皮差异基因主要集中在光合作用、碳水化合物代谢以及氨基酸代谢等通路中,说明在云白1号果皮中,叶绿体发育缺陷导致光合作用及其推进的碳代谢和氮代谢相关进程受到了严重影响;此外,两份材料的种子差异基因主要富集在碳水化合物代谢和脂质降解通路中,表明云白1号种子的碳协调分配方式不同于中茶129,这可能有助于维持白化果实种子的正常生长发育。相关结果可为深入研究茶树果实白化现象提供参考。

关键词: 茶树, 果皮, 白化, 转录组分析

Abstract: Albino pericarp is a rare mutation in tea plants, which has not been reported yet. Recently, among the albino tea mutants preserved in the China National Germplasm Tea Repository, a precious resource ‘Yunbai 1’ with albino pericarp were found. The ultrastructure of the albino pericarp of ‘Yunbai 1’ was compared with the control ‘Zhongcha 129’. The results indicate that compared with the green pericarp of ‘Zhongcha 129’, the plastid structure in albino pericarp cells of ‘Yunbai 1’ was seriously damaged and could not further develop into chloroplasts. Transcriptomic analysis shows that, the differentially expressed genes (DEGs) in pericarps of two cultivars were enriched in the pathways of photosynthesis, carbohydrate metabolism, amino acid metabolism, indicating that the defective chloroplasts in albino pericarp disrupted photosynthetic carbon metabolism and nitrogen metabolism. In addition, numerous DEGs of seeds between two cultivars were enriched in the pathways of carbohydrate metabolism and fatty acid degradation, revealing that the carbon allocation strategy in the seeds of ‘Yunbai 1’ was different from ‘Zhongcha 129’, which might be helpful for maintaining the normal growth and development of seeds. The related results could provide important reference for further study on the albinism of tea fruits.

Key words: tea plant, pericarp, albino, RNA-Seq

中图分类号:

S571.1

汤榕津, 刘浩然, 刘丁丁, 张晨禹, 龚洋, 叶圆圆, 陈杰丹, 陈亮, 马春雷. 茶树白化果皮超微结构观察及分子机制研究[J]. 茶叶科学, 2022, 42(6): 779-790. doi: 10.13305/j.cnki.jts.2022.06.009.

TANG Rongjin, LIU Haoran, LIU Dingding, ZHANG Chenyu, GONG Yang, YE Yuanyuan, CHEN Jiedan, CHEN Liang, MA Chunlei. The Ultrastructure and Molecular Mechanism of Albino Pericarp in Tea Plants[J]. Journal of Tea Science, 2022, 42(6): 779-790. doi: 10.13305/j.cnki.jts.2022.06.009.

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茶树白化果皮超微结构观察及分子机制研究

The Ultrastructure and Molecular Mechanism of Albino Pericarp in Tea Plants

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