植物激素对茶树春季新梢生长发育的调控作用研究

doi:10.13305/j.cnki.jts.2023.03.002

茶叶科学 ›› 2023, Vol. 43 ›› Issue (3): 335-348.doi: 10.13305/j.cnki.jts.2023.03.002

植物激素对茶树春季新梢生长发育的调控作用研究

李聪聪^1,2, 王浩乾^1,2, 叶玙璠^1,2, 陈瑶¹, 任恒泽¹, 李宇腾^1,2, 郝心愿^1,*, 王新超¹, 曹红利^2,3, 岳川^2,3,*

1.中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部特种经济动植物生物学与遗传育种重点实验室,浙江杭州 310008;
2.福建农林大学园艺学院,福建福州 350002;
3.西南大学食品科学学院,重庆 400715

收稿日期:2023-02-15 修回日期:2023-03-20 出版日期:2023-06-15 发布日期:2023-06-29
通讯作者: *haoxy@tricaas.com;yccyyx@163.com
作者简介:李聪聪,女,硕士研究生,主要从事茶树生物技术方面的研究。
基金资助:
国家自然科学基金项目（32172632,31972461）、中国农业科学院基本科研业务费专项（Y2021PT07）、农业科技创新工程（CAAS-ASTIP-2021-TRICAAS）

Study on the Regulation Roles of Plant Hormones on the Growth and Development of Tea Shoots in Spring

LI Congcong^1,2, WANG Haoqian^1,2, YE Yufan^1,2, CHEN Yao¹, REN Hengze¹, LI Yuteng^1,2, HAO Xinyuan^1,*, WANG Xinchao¹, CAO Hongli^2,3, YUE Chuan^2,3,*

1. Tea Research Institute, Chinese Academy of Agriculture Sciences/National Center for Tea Improvement/Key Laboratory of Biology, Genetics and breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China;
2. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
3. College of Food Science, Southwest University, Chongqing 400715, China

Received:2023-02-15 Revised:2023-03-20 Online:2023-06-15 Published:2023-06-29

摘要/Abstract

摘要： 激素在植物生长发育调控中发挥重要作用,为明确不同激素对茶树新梢生长发育的影响,挖掘参与调控这一过程的主要通路和关键基因,以龙井43为试材,在茶树处于萌动期时分别进行100 μmol·L^-1脱落酸（ABA）、100 μmol·L^-1赤霉素（GA₃）和100 μmol·L^-1吲哚-3-乙酸（IAA）喷施处理,观察新梢萌发表型,并对处理后第7天的新梢进行转录组测序分析。结果显示,外源ABA处理抑制新梢生长,处理7 d后芽长极显著短于对照;GA₃和IAA处理则具有促进作用,GA₃处理7 d芽长极显著长于对照,IAA处理14 d芽长极显著长于对照。转录组分析表明,ABA处理新梢中的氨基酸生物合成通路、GA₃处理的氧化磷酸化通路和光合作用通路及IAA处理的类黄酮生物合成通路是差异基因富集的主要通路;植物激素通路、光合作用通路相关的GAI、PSBO2、PSBQ-2和PSBP-1可能是参与新梢生长发育的关键基因。对部分候选基因的表达模式进行实时荧光定量验证,表明转录组分析结果可靠。以上研究明确了激素影响茶树新梢生长发育的主要通路和关键基因,将为全面揭示茶树新梢生长发育调控机制提供理论依据。

关键词: 茶树, 脱落酸, 赤霉素, 吲哚-3-乙酸, 转录组, 生长发育

Abstract: Hormones play important roles in the regulation of plant growth and development. In order to clarify the effects of different hormones on the growth and development of tea shoots in spring, and to identify the main pathways and key genes involved, tea cultivar ‘Longjing 43’ was used as the experimental materials, and treated with 100 μmol·L^-1 ABA, 100 μmol·L^-1 GA₃ and 100 μmol·L^-1 IAA respectively in the sprouting period. The phenotypic characteristics of buds were determined, and the buds at the 7th day after treatments were investigated using RNA-Seq technique. The results show that exogenous ABA treatment inhibited the germination and growth of shoots, and the length of shoots was significantly shorter than the control after 7 days after treatment. On the other hand, GA₃ and IAA treatments had a promoting effect, and the bud length was significantly extended on the 7th day after GA₃treatment and on the 14th day after IAA treatment. RNA-Seq analysis indicates differentially expressed genes were mainly enriched in amino acid biosynthesis pathway under ABA treatment, oxidative phosphorylation pathway and photosynthesis pathway under GA₃ treatment, and flavonoid biosynthesis pathway under IAA treatment. GAI, PSBO2, PSBQ-2 and PSBP-1 related to plant hormone and photosynthesis pathways might be the key genes involved in shoot growth and development. The real-time fluorescence quantitative PCR results of some candidate genes were consistent with the RNA-Seq results. The above studies identified the main pathways and key genes involved in the hormone regulation on tea shoot growth and development, which provided a theoretical basis for deeply revealing the regulation mechanism of tea shoot growth and development.

Key words: Camellia sinensis, ABA, GA₃, IAA, RNA-Seq, growth and development

中图分类号:

李聪聪, 王浩乾, 叶玙璠, 陈瑶, 任恒泽, 李宇腾, 郝心愿, 王新超, 曹红利, 岳川. 植物激素对茶树春季新梢生长发育的调控作用研究[J]. 茶叶科学, 2023, 43(3): 335-348. doi: 10.13305/j.cnki.jts.2023.03.002.

LI Congcong, WANG Haoqian, YE Yufan, CHEN Yao, REN Hengze, LI Yuteng, HAO Xinyuan, WANG Xinchao, CAO Hongli, YUE Chuan. Study on the Regulation Roles of Plant Hormones on the Growth and Development of Tea Shoots in Spring[J]. Journal of Tea Science, 2023, 43(3): 335-348. doi: 10.13305/j.cnki.jts.2023.03.002.

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植物激素对茶树春季新梢生长发育的调控作用研究

Study on the Regulation Roles of Plant Hormones on the Growth and Development of Tea Shoots in Spring

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