不同茶树品种中CsNUDX1基因催化功能、启动子结构及功能分析

doi:10.13305/j.cnki.jts.2023.05.001

摘要/Abstract

摘要： 香叶醇是茶树中重要的萜烯类化合物,在不同茶树品种中的积累存在较大差异。茶树核苷二磷酸水解酶（Nucleoside diphosphate hydrolase,Nudix）基因CsNUDX1-cyto可促进香叶醇及其糖苷在茶树中的积累。为探究该基因在不同茶树品种中的催化功能及调控差异,分析了7个茶树品种中香叶醇的积累差异及CsNUDX1-cyto时空表达变化,同时分析了该基因的催化功能及其启动子结构和功能差异。结果显示,CsNUDX1-cyto表达量与香叶醇含量变化呈显著正相关（r=0.805）;香叶醇在中国变种茶树嫩梢中的含量显著高于阿萨姆变种茶树。农杆菌介导的本氏烟草遗传转化体系表明,不同茶树品种的CsNUDX1-cyto均能促进香叶醇的生物合成。启动子活性分析显示,云抗10号茶树品种中CsNUDX1-cyto的启动子活性较弱;结构分析表明,云抗10号茶树品种CsNUDX1-cyto启动子在转录起始位点-33处有185碱基的序列插入,使得增强元件CAAT-box位于-133处（其他品种CAAT-box均位于-47处）。研究结果表明不同茶树品种中的CsNUDX1-cyto均能够促进香叶醇的生物合成,但启动子区域遗传多样性使得其表达水平有差异。

关键词: 茶树, 香叶醇, NUDX1基因, 功能分析, 启动子分析

Abstract: Geraniol is an important monoterpenoid in tea plants, and its accumulation varies greatly among different tea cultivars. The recent study shows that CsNUDX1-cyto is responsible for the production of geraniol and its glycosides in tea plants. In order to explore the differences in the catalytic function and regulation of CsNUDX1-cyto in different tea cultivars, this study analyzed the differences in the accumulation of geraniol and expression patterns of CsNUDX1-cyto, and analyzed the differences in the catalytic function, promoter structure and function of CsNUDX1-cyto in seven tea cultivars. The result shows that CsNUDX1-cyto expression was positively correlated with geraniol content (r=0.805). The content of geranyl in fresh leaves of Camellia sinensis var. sinensis (CSS) was significantly higher than that in C. sinensis var. assamica (CSA) cultivars. Agrobacterium tumefaciens-mediated genetic transformation system shows that CsNUDX1-cyto of different tea cultivars could promote the biosynthesis of geraniol. Analysis of promoter activity shows that CsNUDX1-cyto promoter had the weakest activity in ‘Yunkang 10’, and the structural analysis shows that the promoter of CsNUDX1-cyto in ‘Yunkang 10’ had an 185 base sequence insertion at the transcription start site -33, making the enhancing element CAAT-box located at -133 (CAAT-boxes in other cultivars were located at -47). The results of this study indicate that CsNUDX1-cyto in different tea cultivars could promote geraniol biosynthesis, but the genetic diversity of the promoter region results in differences in its expression level.

Key words: tea plant, geraniol, NUDX1 gene, function analysis, promoter analysis

中图分类号:

S571.1
Q52

杨霁虹, 周汉琛, 徐玉婕. 不同茶树品种中CsNUDX1基因催化功能、启动子结构及功能分析[J]. 茶叶科学, 2023, 43(5): 621-630. doi: 10.13305/j.cnki.jts.2023.05.001.

YANG Jihong, ZHOU Hanchen, XU Yujie. Catalytic Function, Promoter Structure and Functional Analysis of CsNUDX1-cyto in Different Tea Cultivars[J]. Journal of Tea Science, 2023, 43(5): 621-630. doi: 10.13305/j.cnki.jts.2023.05.001.

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