溴虫氟苯双酰胺影响灰茶尺蠖成虫合成与识别性信息素的能力

doi:10.13305/j.cnki.jts.2024.04.006

摘要/Abstract

摘要： 灰茶尺蠖（Ectropis grisescens Warren）是重要的茶树害虫,广泛分布于我国主要茶产区,干扰其性信息素通讯是防治该类害虫的有效方式。使用亚致死剂量的溴虫氟苯双酰胺处理灰茶尺蠖成虫,利用风洞测试发现雄蛾对性信息素诱芯表现出活跃、起飞、接触行为发生的占比分别下降了13.79%、44.23%、60.00%,且反应发生时间显著延长。进一步的电生理分析发现,处理组雄蛾对0.01βmg·L^-1的性信息素组分Z3,Z6,Z9-18:H的触角电位反应下降了54.57%。另外,当雌蛾受到溴虫氟苯双酰胺处理,GC-MS分析结果显示,雌蛾性腺性信息素主成分Z3,Z6,Z9-18:H和Z3,epo6,Z9-18:H的含量分别下降了21.76%和34.71%,雌蛾合成性信息素的能力显著削弱。雌蛾经溴虫氟苯双酰胺处理,参与性信息素合成的潜在基因Egri-CYP340BD1表达量显著下降。研究结果表明,溴虫氟苯双酰胺处理能显著干扰灰茶尺蠖成虫的性信息素通讯,将为茶园尺蠖类害虫行为调控提供技术参考。

关键词: 灰茶尺蠖, 溴虫氟苯双酰胺, 性信息素识别, 行为调控

Abstract: The tea grey geometrid Ectropis grisescens Warren is a devastating chewing pest in tea plantations throughout China, and interfering with the sex pheromone recognition communication between female and male moths becomes an effective method to manage this insect pest. In the present study, the sublethal dose of broflanilide was used to treat adult E. grisescens. The results of wind tunnel tests reveal that the broflanilide-treated male adults showed a declined percentage of the behavioral responses, including excitation, oriented flight, and source contact. However, the corresponding durations of behavioral responses in male moths were significantly increased. Further studies using electrophysiological assays demonstrate that the electroantennogram responses of broflanilide-treated male moths to the sex pheromone of Z3,Z6,Z9-18:H at 0.01 mg·mL^-1 were decreased by 54.57%. Besides, when female moths were treated with a sublethal dose of broflanilide, the gas chromatography-mass spectrometry (GC-MS) analyses demonstrates that the major sex pheromone components, Z3,Z6,Z9-18:H and Z3,epo6,Z9-18:H, in the female pheromone gland were decreased by 21.76% and 34.71% respectively compared with the control. This result reveals the suppression of sex pheromone biosynthesis in broflanilide-treated female moths. Further study by qRT-PCR analysis reveals that a cytochrome P450 monooxygenase of Egri-CYP340BD1 enriched in pheromone gland was significantly up-regulated in broflanilide-treated female moths. Taken together, this study demonstrates that broflanilide treatment would manipulate the courtship of this insect pest, and the results would contribute to the design of insect-behavior-modifying technology in novel pest management.

Key words: Ectropis grisescens, broflanilide, sex pheromone recognition, behavior modification

中图分类号:

徐长霞, 罗宗秀, 马龙. 溴虫氟苯双酰胺影响灰茶尺蠖成虫合成与识别性信息素的能力[J]. 茶叶科学, 2024, 44(4): 618-626. doi: 10.13305/j.cnki.jts.2024.04.006.

XU Changxia, LUO Zongxiu, MA Long. Effects of Broflanilide on the Biosynthesis and Recognition of Sex Pheromone in the Tea Grey Geometrid Ectropis grisescens[J]. Journal of Tea Science, 2024, 44(4): 618-626. doi: 10.13305/j.cnki.jts.2024.04.006.

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