钾营养对茶树EGCG生物合成的调控作用研究

doi:10.13305/j.cnki.jts.2024.06.012

茶叶科学 ›› 2024, Vol. 44 ›› Issue (6): 887-900.doi: 10.13305/j.cnki.jts.2024.06.012

钾营养对茶树EGCG生物合成的调控作用研究

杨楠^1,3, 李转^1,3, 刘玫辰^1,3, 马骏杰^1,3, 石云桃^1,3, 魏湘凝^1,3, 林阳顺², 毛宇源^1,3,*, 高水练^1,2,3,*

1.福建农林大学园艺学院/安溪茶学院,福建福州 350002;
2.福建日春实业有限公司泉州市特级人才创新实验室,福建泉州 362000;
3.福建省高校茶树绿色栽培与加工协同创新中心,福建泉州 362406

收稿日期:2024-07-14 修回日期:2024-09-23 出版日期:2024-12-15 发布日期:2025-01-08
通讯作者: *772862359@qq.com;gaoshuilian@126.com
作者简介:杨楠,女,硕士研究生,从事茶叶功能成分化学与综合利用研究。
基金资助:
福建日春实业有限公司泉州市特级人才创新实验室创新基金项目（RCTL2024001）、福建农林大学科技创新专项基金项目（KFB22109XA）、福建张天福茶叶发展基金会科技创新基金项目（FJZTF01）

Studies on the Regulation of EGCG Biosynthesis in Tea Plants by Potassium Nutrition

YANG Nan^1,3, LI Zhuan^1,3, LIU Meichen^1,3, MA Junjie^1,3, SHI Yuntao^1,3, WEI Xiangning^1,3, LIN Yangshun², MAO Yuyuan^1,3,*, GAO Shuilian^1,2,3,*

1. College of Horticulture & Anxi College of Tea Science, Fujian Agriculture Forestry University, Fuzhou 350002, China;
2. Quanzhou Special Talent Innovation Laboratory of Fujian Richun Industry Co., Ltd., Quanzhou 362000, China;
3. Fujian Collaborative Innovation Center for Green Cultivation and Processing of Tea Tree in Colleges and Universities, Quanzhou 362406, China

Received:2024-07-14 Revised:2024-09-23 Online:2024-12-15 Published:2025-01-08

摘要/Abstract

摘要： 表没食子儿茶素没食子酸酯（EGCG）是茶叶中重要的滋味和保健功能成分。前人已发现钾营养影响茶树EGCG的生物合成,而其生物合成的调控作用机制目前尚不明确。以黄棪一年生茶苗为试验对象,设置5个处理组（K1~K5）,即浇用K₂SO₄的浓度依次为0.4、0.6、0.8、1.0、1.2 mmol∙L^-1。转录组学和代谢组学联合分析结果表明,低钾处理（K1）的茶树新梢中黄酮含量显著积累,EGCG含量达到最高水平,与高钾处理（K5）相比差异达显著水平;EGCG合成路径上的相关代谢物苯丙氨酸、肉桂酸与对香豆酸在K5处理中上调,而类黄酮途径的下游代谢产物（二氢槲皮素、二氢杨梅素、无色飞燕草色素、表没食子儿茶素）则在K1、K2处理中上调。在钾营养的影响下,EGCG的生物合成受到一系列结构基因CsCHI、F3′5′H、CsF3H（CSS0019002）、CsANS、CsANR、Csaro DE、CsSCPL和转录因子（MYB306）的正向调控以及CsPAL、CsC4H、Cs4CL、CsCHS、CsF3H（CSS0016177）、CsDFR（CSS0011557）和转录因子（NAC83）的负向调控。钾营养会通过影响茶树中关键基因的表达对EGCG的合成进行调控,从而影响EGCG的含量。研究结果为钾营养调控茶树的EGCG生物合成提供了科学依据。

关键词: 钾, 表没食子儿茶素没食子酸酯, 生物合成, 茶树

Abstract: Epigallocatechin gallate (EGCG) is an important flavor and health functional component in tea. Previous studies have found that EGCG biosynthesis in tea plants is affected by potassium nutrition, but the regulatory mechanism of its biosynthesis is currently unclear. This study used one year old tea seedlings of Huangdan as the experimental object, and set up 5 treatment groups (K1-K5), with K₂SO₄ concentrations of 0.4, 0.6, 0.8, 1.0 mmol∙L^-1 and 1.2 mmol∙L^-1 for irrigation, respectively. The joint analysis of transcriptomics and metabolomics shows that, under low-potassium treatment (K1), the flavonoid contents in the new shoots of tea plants accumulated significantly and the EGCG content reached the highest level, and the difference reached a significant level compared with that of the high potassium treatment (K5). The related metabolites of phenylalanine, cinnamic acid and p-coumaric acid on the EGCG synthesis pathway were up-regulated in the K4 or K5 treatments, whereas the downstream metabolites of the flavonoid pathway (dihydroquercetin, dihydromyricetin, colorless delphinidin pigment and epigallocatechin) were up-regulated in the K1 and K2 treatments. Under the influence of potassium nutrition, EGCG biosynthesis was positively regulated by a series of structural genes CsCHI, F3′5′H, CsF3H (CSS0019002), CsANS, CsANR, Csaro DE, CsSCPL, and transcription factor (MYB306), as well as negatively regulated by CsPAL, CsC4H, Cs4CL, CsCHS, CsF3H (CSS0016177), CsDFR (CSS0011557) and transcription factor (NAC83). It is thus clear that potassium nutrition regulates EGCG synthesis by affecting the expressions of key genes in tea plants, thereby affecting EGCG content. This study provided a scientific basis for the regulation of EGCG biosynthesis in tea plants by potassium nutrition.

Key words: potassium, EGCG, biosynthesis, tea plant

中图分类号:

S571.1
Q946

杨楠, 李转, 刘玫辰, 马骏杰, 石云桃, 魏湘凝, 林阳顺, 毛宇源, 高水练. 钾营养对茶树EGCG生物合成的调控作用研究[J]. 茶叶科学, 2024, 44(6): 887-900. doi: 10.13305/j.cnki.jts.2024.06.012.

YANG Nan, LI Zhuan, LIU Meichen, MA Junjie, SHI Yuntao, WEI Xiangning, LIN Yangshun, MAO Yuyuan, GAO Shuilian. Studies on the Regulation of EGCG Biosynthesis in Tea Plants by Potassium Nutrition[J]. Journal of Tea Science, 2024, 44(6): 887-900. doi: 10.13305/j.cnki.jts.2024.06.012.

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钾营养对茶树EGCG生物合成的调控作用研究

Studies on the Regulation of EGCG Biosynthesis in Tea Plants by Potassium Nutrition

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