(+)-儿茶素缓解大鼠低氧性肺动脉高压的作用和机制

doi:10.13305/j.cnki.jts.2019.01.006

茶叶科学 ›› 2019, Vol. 39 ›› Issue (1): 55-62.doi: 10.13305/j.cnki.jts.2019.01.006

(+)-儿茶素缓解大鼠低氧性肺动脉高压的作用和机制

颜俊杰¹, 陈方政², 陈罗薇², 王恒³, 黄静雯¹, 金陆飞², 徐于惠¹, 袁琳波^4,*

1. 温州医科大学药学院,浙江温州 325000;
2. 温州医科大学第一临床学院,浙江温州 325000;
3. 温州医科大学眼视光学院,浙江温州 325000;
4. 温州医科大学生理学教研室浙江温州 325000

收稿日期:2018-05-04 修回日期:2018-06-27 出版日期:2019-02-15 发布日期:2019-07-17
作者简介:颜俊杰,男,在读本科生,药学;Email：395998611@qq.com。
基金资助:
浙江省大学生科技创新活动计划暨新苗人才计划（2017R413040）、2017年温州医科大学本专科学生科研立项课题（wyx2017101104）

The Effect and Mechanism of (+)-Catechins on Relieving Hypoxic Pulmonary Hypertension in Rats

YAN Junjie¹, CHEN Fangzheng², CHEN Luowei², WANG Heng³, HUANG Jingwen¹, JIN Lufei², XU Yuhui¹, YUAN Linbo^4,*

1. The pharmacology School, Wenzhou 325000, China;
2. The First Clinical School, Wenzhou 325000, China;
3. The Ophthalmology and Optometry School, Wenzhou 325000, China;
4 The Department of Physiology, Wenzhou Medical University, Wenzhou 325000, China

Received:2018-05-04 Revised:2018-06-27 Online:2019-02-15 Published:2019-07-17

摘要/Abstract

摘要： 研究了(+)-儿茶素对SD大鼠低氧性肺动脉高压（PAH）的影响及其机制。采用27只SD雄性大鼠按随机数字表法分为3组（对照组、缺氧模型组、(+)-儿茶素干预组）。通过检测大鼠平均肺动脉压（Mean pulmonary arterial pressure,mPAP）和肺血管阻力（Pulmonary vascular resistance,PVR）反应体内肺血流动力学变化;处死大鼠后测算右心室肥厚指数（Right ventricle hypertrophy index,RVHI）及肺动脉血管壁厚度和外周血管直径的比值（Vascular wall thickness of outer circumference ratio,WT%）;WB检测肺动脉内皮细胞一氧化氮合酶（eNOS）,用NO试剂盒检测NO表达量;用CCK-8法检测肺动脉平滑肌细胞增殖以确定细胞活力,利用WB检测分析肺动脉平滑肌细胞钙敏感受体（CaSR）、荧光探针法检测细胞内总Ca²⁺的浓度。研究结果表明,与缺氧模型组相比,(+)-儿茶素能够显著减缓肺缺氧诱导的肺动脉压力升高（n=9,P<0.05;）;通过逆转缺氧引起的肺内皮细胞一氧化氮合酶（eNOS）显著减少（n=3,P<0.05）和NO合成显著减少（n=5,P<0.05）,达到抑制肺动脉内皮血管收缩作用;通过下调缺氧诱导的平滑肌细胞内CaSR表达（n=3,P<0.05）,逆转缺氧的升钙效应（n=27,P<0.05）,达到抑制平滑肌细胞增殖的作用（n=30,P<0.05）。综上可见,(+)-儿茶素通过抑制肺血管收缩和血管平滑肌细胞增殖来缓解缺氧诱导的PAH,为PAH的治疗方法提供了新思路。

关键词: (+)-儿茶素, 肺动脉高压, 钙敏感受体, 一氧化氮

Abstract: To study the effects and mechanism of (+)-catechins ((+)-C) on hypoxia pulmonary arterial hypertension (PAH) in rats, twenty seven SD male rats were randomly divided into 3 groups with equalnumber (control group, hypoxia group and hypoxia+(+)-C group). Mean pulmonary arterial pressure (mPAP), pulmonary vascular resistance (PVR) were measured to indicate the hemodynamic changes in vivo. After executing the rats, the right ventricle hypertrophy index (RVHI) and the vascular wall thickness of outer circumference ratio (WT%) were calculated. The expression of nitric oxide synthase (eNOS) and NO in pulmonary artery endothelial cells was analyzed by WB and NO kit respectively. Cellular viability of pulmonary arterial smooth muscle cell was determined by CCK-8 assay. The expression of calcium sensing receptor (CaSR) and total intracellular Ca²⁺ were analyzed by WB and fluorescent probe. The results showed that (+)-C could decrease the pulmonary arterial pressure induced by hypoxia (n=9, P＜0.05). Increase eNOS in pulmonary endothelial cells (n=3, P＜0.05) and increase NO synthesis in pulmonary endothelial cells (n=5, P＜0.05). The expression of CaSR in smooth muscle cells induced by hypoxia was inhibited (n=3, P＜0.05), while the calcium concentration was induced by (+)-C (n＝27, P＜0.05). Vascular smooth muscle cell proliferation was inhibited by (+)-C (n=30, P＜0.05). In conclusion, (+)-C can alleviate hypoxia induced PAH by inhibiting pulmonary vasoconstriction and vascular smooth muscle cell proliferation, providing a new approach for the treatment of PAH.

Key words: (+)-C, pulmonary arterial hypertension, calcium sensing receptor, nitric oxide

中图分类号:

颜俊杰, 陈方政, 陈罗薇, 王恒, 黄静雯, 金陆飞, 徐于惠, 袁琳波. (+)-儿茶素缓解大鼠低氧性肺动脉高压的作用和机制[J]. 茶叶科学, 2019, 39(1): 55-62. doi: 10.13305/j.cnki.jts.2019.01.006.

YAN Junjie, CHEN Fangzheng, CHEN Luowei, WANG Heng, HUANG Jingwen, JIN Lufei, XU Yuhui, YUAN Linbo. The Effect and Mechanism of (+)-Catechins on Relieving Hypoxic Pulmonary Hypertension in Rats[J]. Journal of Tea Science, 2019, 39(1): 55-62. doi: 10.13305/j.cnki.jts.2019.01.006.

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(+)-儿茶素缓解大鼠低氧性肺动脉高压的作用和机制

The Effect and Mechanism of (+)-Catechins on Relieving Hypoxic Pulmonary Hypertension in Rats

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