茶氨酸合成酶基因的SNP挖掘和遗传定位

摘要/Abstract

摘要： 茶氨酸合成酶（Theanine synthetase, TS）基因是茶树茶氨酸代谢过程中的关键酶基因。本研究以氨基酸含量差异明显的亲本及其杂交所得F₁子代为研究材料,克隆TS基因的cDNA序列,挖掘其SNPs位点,并成功将杂合SNP位点定位在遗传连锁群上。研究结果显示,通过序列比对在亲本间检测到3个SNPs,验证得到1个杂合的位点SNP735。将此位点成功转化为dCAPS标记,该标记在子代中的基因型分离比为1∶1,利用该群体已构建的茶树遗传图谱进行遗传定位,将dCAPS标记定位在连锁群LG03上,相邻标记为TM299和TM517。联合此标记及其相邻标记与游离氨基酸总含量和茶氨酸含量进行统计分析,表明具有显著的相关性。

关键词: 茶树, TS, SNP, dCAPS标记, 定位, 茶氨酸

Abstract: The theanine synthetase (TS) gene is considered as the key functional gene for the synthesis of theanine in tea plant. In this study, an F₁ segregating population and its parents were used to detect single nucleotide polymorphism (SNP) in the TS gene for genetic mapping. According to the sequence alignment between the parents, three SNPs were found, and SNP735 was identified to be heterozygous in YS. SNP735 was subsequently transformed into dCAPS marker and used for genotyping in the F₁ population. The results showed that the segregation ratio of alleles at this SNP locus was close to 1:1. The dCAPS marker was mapped to LG03 at a position between TM299 and TM517 in the tea genetic map. Meanwhile, significant correlations between the dCAPS marker and the content of theanine and total amino acid were identified in this study.

Key words: tea plant, TS, SNP, dCAPS marker, mapping, theanine

中图分类号:

李小杰, 马建强, 姚明哲, 陈亮. 茶氨酸合成酶基因的SNP挖掘和遗传定位[J]. 茶叶科学, 2017, 37(3): 251-257.

LI Xiaojie, MA Jianqiang, YAO Mingzhe, CHEN Liang. SNP Detection and Mapping of Theanine Synthetase Gene in Tea Plant[J]. Journal of Tea Science, 2017, 37(3): 251-257.

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