顶空固相微萃取结合GC/MS分析普洱大叶种乔木茶花香气成分

doi:10.13305/j.cnki.jts.2016.02.008

摘要/Abstract

摘要： 运用全自动顶空固相微萃取技术（Headspace solid-phase microextraction,HS-SPME）对云南普洱地区生产的大叶种乔木茶花样品中的挥发性成分进行提取,并采用气相色谱-质谱技术（Gas chromatography-mass spectrometry, GC/MS）进行定性定量分析;同时与该类茶树制成的普洱生茶的挥发性成分进行了比较。结果表明,从普洱大叶种乔木茶花和普洱生茶中鉴定出挥发性成分分别为9种和67种,主要以碳氢化合物和醇类挥发性化合物为主。通过对比发现,普洱大叶种乔木茶花中的挥发性成分均存在于普洱生茶的挥发性成分之中,这些成分均具有一定香气特性。本研究表明,普洱大叶种乔木茶花可作为独立饮品,有类似于茶叶的香味感受,或者可与茶叶混合以提高或改善茶叶产品的香气风味。

关键词: 茶花, 普洱生茶, 顶空固相微萃取（HS-SPME）, 挥发性成分, 气相色谱-质谱（GC/MS）

Abstract: The volatile components of tea flower (Camellia sinensis var. assamica), which was collected from tea garden in Pu-erh district, were extracted by fully automated headspace solid-phase microextraction (HS-SPME), identified by gas chromatography-mass spectrometry (GC/MS), and compared with the volatile components of Pu-erh green tea from same tea garden. The results showed that 9 and 67 kinds of volatile components were identified in tea flower and Pu-erh raw tea, respectively. Among them, hydrocarbons and alcohols were two major volatile components. Comparison showed that all volatile components of the tea flower were also found in Pu-erh green tea. Our results suggests that the tea flower may have similar aroma to tea leaves, and can also be mixed with tea leaves to improve the aromatic flavor.

Key words: tea flower, Pu-erh raw tea, HS-SPME, volatile components, GC/MS

中图分类号:

TS272
Q946.8

王晨, 吕世懂, 廉明, 孟庆雄. 顶空固相微萃取结合GC/MS分析普洱大叶种乔木茶花香气成分[J]. 茶叶科学, 2016, 36(2): 175-183. doi: 10.13305/j.cnki.jts.2016.02.008.

WANG Chen, LÜ Shidong, LIAN Ming, MENG Qingxiong. Analysis of Aroma Components in Big-leaf Tea Plant (Camellia sinensis var. assamica) Flower Using Headspace Solid-phase Microextraction Coupled with GC/MS[J]. Journal of Tea Science, 2016, 36(2): 175-183. doi: 10.13305/j.cnki.jts.2016.02.008.

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