普洱茶对非酒精性脂肪肝大鼠肠道脂肪吸收及粘膜屏障的干预研究

doi:10.13305/j.cnki.jts.2016.03.002

茶叶科学 ›› 2016, Vol. 36 ›› Issue (3): 237-244.doi: 10.13305/j.cnki.jts.2016.03.002

普洱茶对非酒精性脂肪肝大鼠肠道脂肪吸收及粘膜屏障的干预研究

朱晟易¹, 童钰铃¹, 赵奕¹, 吕海鹏², 林智², 沈国丽¹, 孙婷¹, 宋震亚^1,*

1. 浙江大学医学院附属第二医院,浙江杭州 310009;
2. 农业部茶树生物学与资源利用重点实验室,中国农业科学院茶叶研究所,浙江杭州 310008

收稿日期:2015-12-21 出版日期:2016-06-15 发布日期:2019-08-23
通讯作者: *,songzy@medmail.com.cn
作者简介:朱晟易,男,硕士研究生,主要从事非酒精性脂肪性肝病早期预防和治疗的研究。
基金资助:
中医药重点研究计划（2012ZZ011）

Effect of Pu-erh Tea on Long Chain Fatty Acid Metabolism and Expression of Tight Junction Proteins in the Rat Model of Non-alcoholic Fatty Liver Disease

ZHU Shengyi¹, TONG Yuling¹, ZHAO Yi¹, LÜ Haipeng², LIN Zhi², SHEN Guoli¹, SUN Ting¹, SONG Zhenya^1,*

1. The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou 310009, China;
2. Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China

Received:2015-12-21 Online:2016-06-15 Published:2019-08-23

摘要/Abstract

摘要： 研究了普洱茶调控非酒精性脂肪肝大鼠肠道对长链脂肪酸的吸收,并探讨了其可能机制。将36只SD大鼠随机分成正常对照组、脂肪肝模型组和普洱茶干预组,每组12只。实验第8周末处死所有大鼠,测定大鼠体质量、肝指数及血清血脂水平;采用定量PCR测定大鼠肠道黏膜脂质吸收相关蛋白细胞分化抗原36（Cluster of differentiation 36,CD36）、紧密连接相关蛋白如闭锁蛋白（Occludin）、紧密连接蛋白（Zonula occludens 1,ZO-1）和肿瘤坏死因子α（Tumor necrosis factor-α, TNF-α）基因表达水平。研究结果表明,与模型组相比,普洱茶组大鼠体质量和血清LDL-C水平分别下降了16.12%和42.59%;此外,普洱茶组大鼠小肠组织CD36、TNF-α表达水平明显降低,而Occludin、ZO-1表达水平明显升高。研究发现,普洱茶对NAFLD大鼠肠道长链脂肪酸吸收及肠道紧密连接蛋白有一定程度的调控作用,尤其是对NAFLD早期发生发展中游离脂肪酸过度沉积的“第一次打击”有防治价值。

关键词: 普洱茶, 非酒精性脂肪性肝病, 脂质代谢, 紧密连接

Abstract: In this study, the influence of Pu-erh tea on the absorption of long chain fatty acids in non-alcoholic fatty liver disease (NAFLD) rats was analyzed. Moreover, the possible interventional mechanism was also discussed. Thirty-six SD rats were randomly divided into three groups, normal group (NG), NAFLD group, and Pu-erh tea group (PTG), 12 rats for each group. All rats were killed after 8 weeks. Rat weight, liver index, serum lipid level, intestinal lipid absorption protein (CD36), tight junction proteins (Occludin, ZO-1), and TNF-α mRNA were measured.

Key words: Pu-erh tea, nonalcoholic fatty liver disease (NAFLD), lipid metabolism, tight junction

中图分类号:

朱晟易, 童钰铃, 赵奕, 吕海鹏, 林智, 沈国丽, 孙婷, 宋震亚. 普洱茶对非酒精性脂肪肝大鼠肠道脂肪吸收及粘膜屏障的干预研究[J]. 茶叶科学, 2016, 36(3): 237-244. doi: 10.13305/j.cnki.jts.2016.03.002.

ZHU Shengyi, TONG Yuling, ZHAO Yi, LÜ Haipeng, LIN Zhi, SHEN Guoli, SUN Ting, SONG Zhenya. Effect of Pu-erh Tea on Long Chain Fatty Acid Metabolism and Expression of Tight Junction Proteins in the Rat Model of Non-alcoholic Fatty Liver Disease[J]. Journal of Tea Science, 2016, 36(3): 237-244. doi: 10.13305/j.cnki.jts.2016.03.002.

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普洱茶对非酒精性脂肪肝大鼠肠道脂肪吸收及粘膜屏障的干预研究

Effect of Pu-erh Tea on Long Chain Fatty Acid Metabolism and Expression of Tight Junction Proteins in the Rat Model of Non-alcoholic Fatty Liver Disease

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