普洱茶茶褐素对大鼠肠道菌群的影响

doi:10.13305/j.cnki.jts.2016.03.005

摘要/Abstract

摘要： 本研究以普洱茶茶褐素（分子量>50 kDa,TB）为研究对象,通过灌胃茶褐素后分析大鼠体内微生物变化,揭示普洱茶茶褐素对肠道菌群的影响。结果显示,茶褐素能显著改善抗生素的脱污染作用,调整肠道菌群失调,且具有促进大鼠肠道乳酸杆菌、双歧杆菌增殖,抑制大肠杆菌、肠球菌生长的作用,并随时间延长,效果愈加明显;而微生物的大量繁殖可加速茶褐素的分解代谢。

关键词: 普洱茶茶褐素, 肠道菌群, 代谢, 大鼠

Abstract: In this study, theabrownin (Mw>50 kDa, TB) extracted from pu-erh tea was used as experimental material, and the effects of theabrownin to the intestinal flora of rat was studied. The results showed that the theabrownin had remarkable effects on the intestinal flora in rats. TB could significantly improve the de-pollution of antibiotics and adjust the intestinal flora imbalance. It was found that TB could promote the proliferation of Bifidobacterium and Lactobacillus and inhibit the proliferation of Escherichia coli and Enterococcus. The effects are more obvious with increasing culture time. In turn, microbes proliferate can accelerate the catabolism of theabrowns.

Key words: Pu-erh tea theabrownin, intestinal flora, metabolic, rat

中图分类号:

岳随娟, 刘建, 龚加顺. 普洱茶茶褐素对大鼠肠道菌群的影响[J]. 茶叶科学, 2016, 36(3): 261-267. doi: 10.13305/j.cnki.jts.2016.03.005.

YUE Suijuan, LIU Jian, GONG Jiashun. Effects of Theabrownin Extracted from Pu-erh Tea on the Intestinal Flora[J]. Journal of Tea Science, 2016, 36(3): 261-267. doi: 10.13305/j.cnki.jts.2016.03.005.

参考文献 24

[1]	蔡新, 张理珉, 杨善禧, 等. GB/T 22111—2008 地理标志产品普洱茶[S]. 北京: 中国标准出版社, 2008: 1-5.
[2]	薛志强, 李亚莉, 秘鸣, 等. 普洱茶保健功效研究进展[J]. 中国保健营养, 2012, 8(52): 2449-2451.
[3]	蒋成砚, 谢昆, 薛春丽, 等. 普洱茶多糖增强免疫功能研究[J]. 江苏农业科学,2012, 40(1): 257-258.
[4]	王秋萍, 龚加顺, 邹莎莎. 普洱茶发酵阶段色泽的变化及其与品质的关系[J]. 农业工程学报, 2010, 26(SuPP. l): 394-399.
[5]	张新富, 龚加顺, 周红杰, 等. 云南普洱茶中多酚类物质与品质的关系研究[J]. 食品科学, 2008, 29(4): 230-233.
[6]	秦谊, 龚加顺, 张惠芬, 等. 普洱茶茶褐素提取工艺及理化性质的初步研究[J]. 林产化学与工业, 2009, 29(5): 95-98.
[7]	谭超, 彭春秀, 高斌, 等. 普洱茶茶褐素类主要组分特征及光谱学性质研究[J]. 光谱学与光谱分析, 2012, 32(4): 1051-1056.
[8]	Gong JS, Peng CX, Chen T, et al.Effects of theabrownin from Pu-erh tea on the metabolism of serum lipids in rats: mechanism of action[J]. J Food Sci, 2010, 75(6): H182-H189.
[9]	高斌, 彭春秀, 龚加顺, 等. 普洱茶茶褐素对大鼠激素敏感性脂肪酶活性及其mRNA表达的影响[J]. 营养学报, 2010, 32(4): 362-366.
[10]	Madsen C.Functional foods in Europe. International developments in science and health claims[J]. Ann Nutr Metab, 2007, 51(3): 298-299.
[11]	Siro I, E Kapolna, B Kapolna, et al. Functional food Product development, marketing and consumer acceptance-a review[J]. Appetite, 2008, 51(3): 456-467.
[12]	Okubo T, Ishihara N, Oura A, et al.In vivo effects of tea polyphenol intake on human intestinal microflora and metabolism[J]. Biosci Biotech Biochem, 1992, 56(4): 588-591.
[13]	苗明三. 试验动物和动物试验技术[M]. 北京: 中国中医药出版社, 1997: 170-173.
[14]	施新猷. 动物试验方法[M]. 北京: 人民卫生出版社, 1980: 118-119.
[15]	中华人民共和国卫生部. 保健食品检验与评价技术规范[S]. 2003.
[16]	王力宁, 蔡晓静, 黄小琪, 等. 抗复感合剂对抗生素脱污染小鼠肠道菌群影响的研究[J]. 广西中医药, 2006, 29(6): 46-49.
[17]	龚加顺, 陈文品, 周红杰, 等. 云南普洱茶特征成分的功能与毒理学评价[J]. 茶叶科学, 2007, 27(3): 201-210.
[18]	敬明武, 葛宇杰, 刘科亮. 茶色素胶囊对大鼠辅助调节血脂的研究[J]. 中国现代医生, 2007, 45(5): 18-19.
[19]	骆非. 用抗生素使成熟小鼠正常菌丛无菌化的方法[J]. 微生物学免疫学译刊, 1987(S1).
[20]	范明远. 康白论文集[M]. 北京: 中国微生态学杂志社,1989: 101-110.
[21]	黄秀梨, 辛明秀, 夏立秋, 等. 微生物学实验指导[M]. 北京: 高等教育出版社, 2008: 53-60.
[22]	Jia W, Li H, Zhao L, et al.Gut microbiota: a potential new territory for drug targeting[J]. Nat Rev Drug Discov, 2008(7): 123-129.
[23]	Nicholson JK, Wilson ID.Understanding ‘global’ systems biology: metabonomies and the continuum of metabclism[J]. Nat Rev Drug Discov, 2003(2): 668-676.
[24]	申瑞玲, 王章存, 姚惠源. 燕麦β-葡聚糖对小鼠肠道菌群的影响[J]. 食品科学, 2005, 26(2): 208-212.