茶叶香气成分中芳樟醇旋光异构体的分析

doi:10.13305/j.cnki.jts.2015.02.005

茶叶科学 ›› 2015, Vol. 35 ›› Issue (2): 137-144.doi: 10.13305/j.cnki.jts.2015.02.005

茶叶香气成分中芳樟醇旋光异构体的分析

杨停^1,2, 朱荫¹, 吕海鹏^1,*, 马成英¹, 张悦¹, 施江^1,2, 刘爽^1,2, 林智^1,*

1. 农业部茶树生物学与资源利用重点实验室;中国农业科学院茶叶研究所,杭州 310008;
2. 中国农业科学院研究生院,北京 100081

收稿日期:2014-09-18 修回日期:2014-12-02 出版日期:2015-04-15 发布日期:2019-08-23
通讯作者: *lvhaipeng@tricaas.com,linz@tricaas.com
作者简介:杨停,女,硕士研究生,主要从事茶叶加工品质化学研究。
基金资助:
浙江省自然科学基金（LY14C160002）、现代农业产业技术体系建设专项资金（CARS-23）、中国农业科学院科技创新工程（CAAS-ASTIP-2014-TRICAAS）

Analysis of Optical Isomers of Linalool in Tea Aromatic Components

YANG Ting^1,2, ZHU Yin¹, LYU Haipeng^1,*, MA Chengying¹, ZHANG Yue¹, SHI Jiang^1,2, LIU Shuang^1,2, LIN Zhi^1,*

1. Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2014-09-18 Revised:2014-12-02 Online:2015-04-15 Published:2019-08-23

摘要/Abstract

摘要： 芳樟醇是茶叶中含量很高的香气成分之一,具有左旋和右旋两种光学异构体,而这两者有着完全不同的香气品质。本研究利用手性色谱柱,采用顶空固相微萃取-气相色谱法对绿茶和红茶中芳樟醇旋光异构体的比例进行了分析。结果表明,绿茶和红茶中都同时存在3S-(+)-芳樟醇和3R-(-)-芳樟醇两种光学异构体。供试绿茶茶样中3S-(+)-芳樟醇与3R-(-)-芳樟醇相对含量的比值（S/R）介于2.07~14.05,平均比值为5.21。表明供试绿茶样品中芳樟醇以3S-(+)-芳樟醇为主要存在形式。此外,蒸青绿茶、炒青绿茶和烘青绿茶3种不同类型绿茶中芳樟醇的两种旋光异构体的组成比例也存在很大的差异,其中蒸青绿茶中的S/R比值最低（2.72）,烘青绿茶的S/R比值最高（8.05）,炒青绿茶的S/R比值（4.78）介于两者之间。供试红茶茶样中3S-(+)-芳樟醇与3R-(-)-芳樟醇相对含量的比值（S/R）介于0.65~0.82,平均比值为0.74。表明红茶样品中芳樟醇以3R-(-)-芳樟醇为主要存在形式。绿茶和红茶加工过程中3S-(+)-芳樟醇和3R-(-)-芳樟醇相对含量的比例变化研究表明,在绿茶加工过程中,杀青后S/R比值达到最高,为5.78;在红茶加工过程中,发酵2βh后S/R比值达到最高,为1.59。不同茶树品种鲜叶中芳樟醇旋光异构体的S/R比值有很大差异,检测发现有的茶树品种鲜叶中的芳樟醇以3R-(-)-芳樟醇为主,如高芽齐和英红9号等,其余5个茶树品种鲜叶中的芳樟醇则以3S-(+)-芳樟醇为主。

关键词: 绿茶, 红茶, 香气, 芳樟醇, 旋光异构体

Abstract: Linalool is one of the most important tea aroma components with high content, and it has two optical isomers, namely 3R-(-)-Linalool and 3S-(+)-Linalool, both show entirely different aroma qualities. In this study, a method using chiral chromatographic column and based on headspace solid-phase micro-extraction (HS-SPME) was developed to analyze 3R-(-)-Linalool and 3S-(+)-Linalool in green tea and black tea samples. Results showed that 3R-(-)-Linalool and 3S-(+)-Linalool isomers were existed in green and black tea. The ratio of the relative content of 3S-(+)-Linalool to that of 3R-(-)-Linalool in green tea ranged from 2.07 to 14.05, with an average ratio of the relative content of 5.21, while, the ratio varied greatly in steamed green tea, roasted green tea and baking green tea. The ratio of the relative content of 3S-(+)-Linalool and 3R-(-)-Linalool were the highest in baking green tea (8.05), with the lowest in steamed green tea (2.72), and 4.78 in roasted green tea. The ratio of the relative content of 3S-(+)-Linalool to that of 3R-(-)-Linalool in black tea ranged from 0.65 to 0.82, with an average ratio of the relative content of 0.74. The ratio of the two optical isomers during the processing of green and black tea was analyzed as well, with the highest found following de-enzyme stage during the processing of green tea and following 2 h fermentation of black tea (5.78 and 1.59 respectively). Fresh leaves of 12 tea cultivars were also analyzed. Moreover, it varied greatly in different tea cultivars.

Key words: green tea, black tea, aroma, linalool, optical isomers

中图分类号:

杨停, 朱荫, 吕海鹏, 马成英, 张悦, 施江, 刘爽, 林智. 茶叶香气成分中芳樟醇旋光异构体的分析[J]. 茶叶科学, 2015, 35(2): 137-144. doi: 10.13305/j.cnki.jts.2015.02.005.

YANG Ting, ZHU Yin, LYU Haipeng, MA Chengying, ZHANG Yue, SHI Jiang, LIU Shuang, LIN Zhi. Analysis of Optical Isomers of Linalool in Tea Aromatic Components[J]. Journal of Tea Science, 2015, 35(2): 137-144. doi: 10.13305/j.cnki.jts.2015.02.005.

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茶叶香气成分中芳樟醇旋光异构体的分析

Analysis of Optical Isomers of Linalool in Tea Aromatic Components

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