EGCG抑制Nicotine诱导肺腺癌H1299细胞JAK2/STAT3 mRNA的表达研究

doi:10.13305/j.cnki.jts.2015.02.010

茶叶科学 ›› 2015, Vol. 35 ›› Issue (2): 171-178.doi: 10.13305/j.cnki.jts.2015.02.010

EGCG抑制Nicotine诱导肺腺癌H1299细胞JAK2/STAT3 mRNA的表达研究

高静¹, 禹利君^1,*, 李晓焕¹, 高鹏¹, 周清明^2,*

1. 湖南农业大学园艺园林学院茶学系茶学教育部重点实验室,湖南长沙 410128;
2. 湖南农业大学农学院烟草系,湖南长沙 410128

收稿日期:2014-08-13 修回日期:2014-12-08 出版日期:2015-04-15 发布日期:2019-08-23
通讯作者: *
作者简介:高静,女,茶学在读硕士,主要从事茶及其关键功能成分的应用开发研究。
基金资助:
湖南省教育厅重点科学研究项目（12A069）

Research on the Inhibitory Effect of EGCG on Nicotine in Inducing H1299 Cancer Cell JAK2/STAT3 mRNA Expression

GAO Jin¹, YU Lijun^1,*, LI Xiaohuan¹, GAO Peng¹, ZHOU Qingming^2,*

1. Key Lab of Tea Science of Ministry of Education, Tea Science Department, College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China;
2. Tobacco Science Department, College of Agriculture, Hunan Agricultural University, Changsha 410128, China

Received:2014-08-13 Revised:2014-12-08 Online:2015-04-15 Published:2019-08-23

摘要/Abstract

摘要： 为探索EGCG对Nicotine诱导肺癌细胞增殖的抑制作用,本研究通过MTT实验筛选出EGCG、Nicotine对肺腺癌细胞H1299的最佳作用浓度,利用实时荧光定量PCR技术检测EGCG、Nicotine对H1299细胞中Bax、Bcl-2、Jak2和Stat3基因mRNA相对表达量的变化。实验结果表明,EGCG对H1299细胞的半抑制浓度IC₅₀值约为32βμmol·L^-1（24βh）、15βμmol·L^-1（48βh）;1βμmol·L^-1的Nicotine对H1299细胞促增殖作用明显;以15βμmol·L^-1的EGCG预处理H1299细胞24βh可显著下调1βμmol·L^-1 Nicotine的促增殖作用（P<0.05）。1βμmol·L^-1的Nicotine处理H1299细胞可明显降低JAK2/STAT3信号通路中Bax基因mRNA的表达,增加Bcl-2、Jak2、Stat3基因的mRNA表达;15βμmol·L^-1 EGCG预处理H1299细胞可反向调控Nicotine诱导所致JAK2/STAT3信号通路中Jak2、Stat3和Bax、Bcl-2基因表达量的变化,结果具有显著性差异（P<0.05）。由此可知,EGCG对Nicotine诱导的肺腺癌H1299细胞增殖及JAK2/STAT3信号通路中促增殖基因mRNA的表达起抑制作用。

关键词: EGCG, Nicotine, H1299细胞, JAK2/STAT3 mRNA表达

Abstract: In order to explore the inhibitory effect of EGCG on nicotine which induced lung cancer cells proliferation, this study selected the optimal concentrations of EGCG and nicotine on lung adenocarcinoma cancer cell H1299 by MTT experiment, and detected mRNA relative expression levels of Bax, Bcl-2, Jak2 and Stat3 genes in H1299 cells using qRT-PCR technique. Experiment results showed that the IC₅₀ of EGCG on H1299 lung adenocarcinoma cells is about 32βμmol·L^-1 (treated 48βh) and 15βμmol·L^-1 (treated 24βh), nicotine obviously promotes the proliferation of H1299 lung adenocarcinoma cell at 1βμmol·L^-1 concentration treatment. Pretreatment of H1299 cells with 15βμmol·L^-1 EGCG (24βh) can significantly down-regulate the promotion of proliferation induced by 1βμmol·L^-1 Nicotine (P<0.05). Using 1βμmol·L^-1 nicotine to treat H1299 cells has showed that nicotine significantly reduce the mRNA expression level of Bax gene, increase the mRNA expression levels of Bcl-2, Jak2 and Stat3 genes in the JAK2/STAT3 signal pathway. Pretreatment of H1299 cells with 15βμmol·L^-1 EGCG (24βh) can be observed that EGCG significantly retroregulated the mRNA expression levels of Bax,Bcl-2,Jak2 and Stat3 genes in the JAK2/STAT3 signal pathway,and got the results with significant differences (P<0.05). In conclusion,EGCG plays important role in suppressing the survival rates of H1299 cells and mRNA expression level of JAK2/STAT3 signaling pathway induced by nicotine.

Key words: EGCG, nicotine, H1299 cell, JAK2/STAT3 mRNA expressions

中图分类号:

高静, 禹利君, 李晓焕, 高鹏, 周清明. EGCG抑制Nicotine诱导肺腺癌H1299细胞JAK2/STAT3 mRNA的表达研究[J]. 茶叶科学, 2015, 35(2): 171-178. doi: 10.13305/j.cnki.jts.2015.02.010.

GAO Jin, YU Lijun, LI Xiaohuan, GAO Peng, ZHOU Qingming. Research on the Inhibitory Effect of EGCG on Nicotine in Inducing H1299 Cancer Cell JAK2/STAT3 mRNA Expression[J]. Journal of Tea Science, 2015, 35(2): 171-178. doi: 10.13305/j.cnki.jts.2015.02.010.

参考文献

[1] S Park, SH Jee, HR Shin, et al. Attributable fraction of tobacco smoking on cancer using population-based nationwide cancer incidence and mortality data in Korea[J]. BMC Cancer, 2014, 14(1): 406-410.
[2] NL Guo, K Tosun, K Horn. Impact and interactions between smoking and traditional prognostic factors in lung cancer progression[J]. Lung Cancer, 2009, 66(3): 386-392.
[3] J Zhang, JX Ou, CX Bai. Tobacco smoking in China: prevalence, disease burden, challenges and future strategies[J]. Respirology, 2011, 16(8): 1165-1172.
[4] SS Pakhale, K Jayant, SV Bhide. Total particulate matter and nicotine in Indian bidis and cigarettes: a comparative study of standard machine estimates and exposure levels in smokers in Bombay[J]. Indian J Cancer, 1989, 26(4): 227-232.
[5] GD Byrd, JH Robinson, WS Caldwell, et al. Comparison of measured and FTC-predicted nicotine uptake in smokers[J]. Psychopharmacology (Berl), 1995, 122(2): 95-103.
[6] A Iwase, M Aiba, S Kira.Respiratory nicotine absorption in non-smoking females during passive smoking[J]. Int Arch Occup Environ Health, 1991, 63(2): 139-143.
[7] JA Turner, RW Sillett, MW McNicol. Effect of cigar smoking on carboxyhaemoglobin and plasma nicotine concentrations in primary pipe and cigar smokers and ex-cigarette smokers[J]. Br Med J, 1977, 2: 1387-1389.
[8] KC Brown, HE Perry, JK Lau, et al. Nicotine induces the up-regulation of the alpha7-nicotinic receptor(alpha7-nAChR) in human squamous cell lung cancer cells via the Sp1/GATA protein pathway[J]. J Biol Chem, 2013, 288(46): 33049-33059.
[9] X Ma, Y Jia, S Zu, et al. Alpha5 Nicotinic acetylcholine receptor mediates nicotine-induced HIF-1alpha and VEGF expression in non-small cell lung cancer[J]. Toxicol Appl Pharmacol, 2014, 278(2): 172-179.
[10] T Nishioka, J Guo, D Yamamoto, et al. Nicotine, through upregulating pro-survival signaling, cooperates with NNK to promote transformation[J]. J Cell Biochem, 2010, 109(1): 152-161.
[11] H Mai, WS May, F Gao, et al. A functional role for nicotine in Bcl2 phosphorylation and suppression of apoptosis[J]. J Biol Chem, 2003, 278(3): 1886-1891.
[12] VM Adhami, IA Siddiqui, N Ahmad, et al. Oral consumption of green tea polyphenols inhibits insulin-like growth factor-I-induced signaling in an autochthonous mouse model of prostate cancer[J]. Cancer Res, 2004, 64(23): 8715-8722.
[13] YD Jung, MS Kim, BA Shin, et al. EGCG, a major component of green tea, inhibits tumour growth by inhibiting VEGF induction in human colon carcinoma cells[J]. Br J Cancer, 2001, 84(6): 844-850.
[14] 刘礼, 谢纪文, 王熙, 等. EGCG联合不同剂量放疗对人肝癌细胞系HepG2中hTERT表达的影响[J]. 世界华人消化杂志, 2010, 18(18): 1873-1878.
[15] 王江红, 徐莉, 董扬, 等. EGCG联合紫杉醇对肺癌A549细胞增殖、凋亡的影响[J]. 重庆医科大学学报, 2012, 37(4): 306-310.
[16] WY Gong, JF Wu, BJ Liu, et al. Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro[J]. Int J Oncol, 2014, 44(5): 1561-1570.
[17] SH Tu, CY Ku, CT Ho, et al. Tea polyphenol (-)-epigallocatechin-3-gallate inhibits nicotine- and estrogen-induced alpha9-nicotinic acetylcholine receptor upregulation in human breast cancer cells[J]. Mol Nutr Food Res, 2011, 55(3): 455-466.
[18] MJ Kim, HJ Nam, HP Kim, et al. OPB-31121, a novel small molecular inhibitor, disrupts the JAK2/STAT3 pathway and exhibits an antitumor activity in gastric cancer cells[J]. Cancer Lett, 2013, 335(1): 145-152.
[19] U Rozovski, JY Wu, DM Harris, et al. Stimulation of the B-cell receptor activates the JAK2/STAT3 signaling pathway in chronic lymphocytic leukemia cells[J]. Blood, 2014, 123(24): 3797-3802.
[20] RY Liu, Y Zeng, Z Lei, et al. JAK/STAT3 signaling is required for TGF-beta-induced epithelial-mesenchymal transition in lung cancer cells[J]. Int J Oncol, 2014, 44(5): 1643-1651.

EGCG抑制Nicotine诱导肺腺癌H1299细胞JAK2/STAT3 mRNA的表达研究

Research on the Inhibitory Effect of EGCG on Nicotine in Inducing H1299 Cancer Cell JAK2/STAT3 mRNA Expression

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