TF1与EGCG在Caco-2细胞中的吸收规律研究

doi:10.13305/j.cnki.jts.2015.03.005

茶叶科学 ›› 2015, Vol. 35 ›› Issue (3): 233-238.doi: 10.13305/j.cnki.jts.2015.03.005

TF₁与EGCG在Caco-2细胞中的吸收规律研究

孟庆, 屠幼英^*

浙江大学农业与生物技术学院茶学系,浙江杭州,310058

收稿日期:2015-01-14 修回日期:2015-04-13 出版日期:2015-06-15 发布日期:2019-08-23
通讯作者: *youytu@zju.edu.cn
作者简介:孟庆,男,博士研究生,主要从事茶叶生物化学研究。
基金资助:
福建省“2011协同创新中心”中国乌龙茶产业协同创新中心（培育）专项（2013-51）

Study on Absorption of Theaflavin and EGCG in Caco-2 Cell Model

MENG Qing, TU Youying^*

Department of tea science, Zhejiang University, Hangzhou 310058, China

Received:2015-01-14 Revised:2015-04-13 Online:2015-06-15 Published:2019-08-23

摘要/Abstract

摘要： 为了探究茶黄素单体（TF₁）与表没食子儿茶素没食子酸酯（EGCG）在生物体中的吸收规律,本实验建立了体外Caco-2单层细胞模型,模拟小肠对TF₁与EGCG的吸收,并研究了浓度和时间对吸收的影响。结果表明,在10~100βµmol·L^-1范围内,TF₁与EGCG在Caco-2单层细胞模型中的吸收呈现表观渗透系数Papp随浓度增加而上升的规律。但两者的外排率也呈现同样的规律,并且外排率上升的幅度均大于二者吸收率上升的幅度。由于TF₁与EGCG在细胞单层模型中的Papp均小于1×10^-6βcm·s^-1,说明两者都属于难吸收的药物,但是TF₁在Caco-2细胞模型中的吸收率高于EGCG。因其外排比均大于2,说明两者在细胞模型上的外排是被动转运。从吸收时间看,TF₁的外排规律与EGCG一致。

关键词: TF₁, EGCG, Caco-2细胞模型, 吸收规律

Abstract: In order to explore the absorption regularity of TF₁ and EGCG in the organism. This research used the Caco-2 cell monolayer model in vitro to simulate the absorption of TF₁ and EGCG in the small intestine. The influences of concentration and time on the absorption regularity of TF₁ and EGCG in Caco-2 cell monolayer model were investigated. The results illustrated that the absorption of TF₁ and EGCG in Caco-2 model showed that the apparent permeability coefficient raised with the increasing of concentrations of two compound in the range of 10~100βµmol·L^-1. The efflux rates of the TF₁ and EGCG showed the same rules as absorption. However, the increasing range of efflux rate was higher than that of absorption rate. The values of Papp about TF₁ and EGCG in the cell model were lower than 1×10^-6βcm·s^-1, which indicated that both of them belonged to the kind of drugs which were difficult to absorb. However, with comparing the absorption rate, TF₁ was higher than EGCG in this model. Both of efflux transport showed the passive process because the efflux rates of TF₁ and EGCG were larger than 2 and higher than in cell model. Because the efflux regulation of TF₁ was in accordance with EGCG by temporal variation, it suggested that both of them were the substrate of the same efflux protein.

Key words: TF₁, EGCG, Caco-2 model, absorption regularity

中图分类号:

孟庆, 屠幼英. TF₁与EGCG在Caco-2细胞中的吸收规律研究[J]. 茶叶科学, 2015, 35(3): 233-238. doi: 10.13305/j.cnki.jts.2015.03.005.

MENG Qing, TU Youying. Study on Absorption of Theaflavin and EGCG in Caco-2 Cell Model[J]. Journal of Tea Science, 2015, 35(3): 233-238. doi: 10.13305/j.cnki.jts.2015.03.005.

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TF₁与EGCG在Caco-2细胞中的吸收规律研究

Study on Absorption of Theaflavin and EGCG in Caco-2 Cell Model

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