茶黄素与EGCG抑制体内外β-淀粉样蛋白1-42水平及其诱导的神经细胞氧化损伤

doi:10.13305/j.cnki.jts.2016.06.013

茶叶科学 ›› 2016, Vol. 36 ›› Issue (6): 655-662.doi: 10.13305/j.cnki.jts.2016.06.013

• • 上一篇

茶黄素与EGCG抑制体内外β-淀粉样蛋白1-42水平及其诱导的神经细胞氧化损伤

张静¹, 黄建安^1,2,3, 蔡淑娴¹, 易晓芹¹, 刘建军¹, 王英姿¹, 田丽丽¹, 刘仲华^1,2,3,*

1. 湖南农业大学茶学教育部重点实验室,湖南长沙 410128;
2. 国家植物功能成分利用工程技术研究中心,湖南长沙 410128;
3. 植物功能成分利用协同创新中心,湖南长沙 410128

收稿日期:2016-08-08 出版日期:2016-12-15 发布日期:2019-08-26
通讯作者: *,larkin-liu@163.com
作者简介:张静,女,博士,主要从事茶叶功能成分药理研究。
基金资助:
国家茶叶产业技术体系项目（CARS-23-11B）、湖南省植物功能成分利用协同创新项目（HNCR-2014003）、国家自然科学基金项目（31471590）、湖南省研究生科研创新项目（CX2015B245）

Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ₄₂ in vivo and in vitro

ZHANG Jing¹, HUANG Jian'an^1,2,3, CAI Shuxian¹, YI Xiaoqin¹, LIU Jianjun¹, WANG Yingzi¹, TIAN Lili¹, LIU Zhonghua^1,2,3,*

1. Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, China;
2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
3. Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Changsha 410128, China

Received:2016-08-08 Online:2016-12-15 Published:2019-08-26

摘要/Abstract

摘要： 通过模拟生理条件体外实验,将β-淀粉样蛋白1-42（Aβ₄₂）与EGCG和4种茶黄素单体分别按照1︰1和1︰5比例进行孵育,采用硫黄素T荧光检测β-折叠结构的生成量,结果表明,EGCG与茶黄素均能明显降低β-折叠结构生成,从而抑制Aβ₄₂聚集;此外,通过建立20 μmol·L^-1Aβ₄₂诱导人神经母细胞瘤SH-SY5Y细胞损伤模型,将不同浓度的EGCG或茶黄素（10、50、100 μmol·L^-1）处理后,MTT法检测细胞存活率,同时,检测细胞内活性氧（ROS）、谷胱甘肽过氧化物酶（GSH-Px）及丙二醛（MDA）等抗氧化指标,结果表明,EGCG与茶黄素可抑制因Aβ₄₂诱导SH-SY5Y细胞活力下降及氧化损伤。体内实验中,通过10 mg·kg^-1·d^-1的EGCG或茶黄素处理快速老化模型小鼠（SAMP8）,10周后检测小鼠血清Aβ₄₂及晚期糖基化终末产物（AGEs）含量,结果表明,EGCG和茶黄素可显著降低SAMP8小鼠血清中Aβ₄₂及AGEs含量。本研究表明了茶黄素和EGCG可抑制Aβ₄₂聚集纤维化及Aβ₄₂导致的神经氧化损伤,从而对阿尔茨海默病具有一定保护作用。

关键词: EGCG, 茶黄素, β-淀粉样蛋白1-42, 晚期糖基化终末端产物, SH-SY5Y细胞, SAMP8小鼠

Abstract: In this study, amyloid β-protein Aβ₄₂ was incubated with EGCG, TF, TF-3-G, TF-3'-G, TF-DG at the ratio of 1:1 and 1:5 under simulated physiological condition. The contents of β-sheets after incubation were tested by thioflavin T fluorometric assay. It was found that EGCG and theaflavins could suppress the formation of β-sheet structure, thereby inhibiting aggregations of Aβ₄₂. In addition, an SH-SY5Y cell damage model was established and MTT assay was used to examine cell viabilities when 20 μmol·L^-1 Aβ₄₂ was incubated with different concentrations of EGCG and theaflavins (10, 50, 100 μmol·L^-1). Intracellular reactive oxygen species (ROS) levels, antioxidant enzyme activities (GSH-Px) and MDA levels were also measured. The results showed EGCG and theaflavins treatments could alleviate the Aβ₄₂ induced oxidative damage to neural cells by reducing ROS and increasing the activity of GSH-Px. To study the in vivo protective effects of EGCG and theaflavins, SAMP8 mice were treated with 10 mg·kg-1·d^-1 EGCG or theaflavins respectively by gavage for 10 weeks, and the contents of Aβ₄₂ and AGEs in mouse serum were determined. The results showed that EGCG and theaflavins significantly decreased the contents of Aβ₄₂ and AGEs in SAMP8 serum. In summary, EGCG and theaflavins could inhibit the aggregations of Aβ₄₂ and reduce oxidative injury induced by Aβ₄₂ in neural cells, thereby exerting protective effects on AD.

Key words: EGCG, theaflavins, amyloid-β1-42, advanced glycation end products, SH-SY5Y cells, SAMP8 mice

中图分类号:

TS272
Q946.8

张静, 黄建安, 蔡淑娴, 易晓芹, 刘建军, 王英姿, 田丽丽, 刘仲华. 茶黄素与EGCG抑制体内外β-淀粉样蛋白1-42水平及其诱导的神经细胞氧化损伤[J]. 茶叶科学, 2016, 36(6): 655-662. doi: 10.13305/j.cnki.jts.2016.06.013.

ZHANG Jing, HUANG Jian'an, CAI Shuxian, YI Xiaoqin, LIU Jianjun, WANG Yingzi, TIAN Lili, LIU Zhonghua. Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ₄₂ in vivo and in vitro[J]. Journal of Tea Science, 2016, 36(6): 655-662. doi: 10.13305/j.cnki.jts.2016.06.013.

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茶黄素与EGCG抑制体内外β-淀粉样蛋白1-42水平及其诱导的神经细胞氧化损伤

Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ₄₂ in vivo and in vitro

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茶黄素与EGCG抑制体内外β-淀粉样蛋白1-42水平及其诱导的神经细胞氧化损伤

Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ42 in vivo and in vitro

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Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ₄₂ in vivo and in vitro