外源油菜素内酯对茶树光合特性的影响

doi:10.13305/j.cnki.jts.2015.06.005

摘要/Abstract

摘要： 24-表油菜素内酯（EBR）是一种重要的植物激素,在植物对多种逆境的抵抗中发挥着重要的作用。本试验以田间龙井43、清明早和香菇寮白毫茶树品种为试验材料,研究了外源EBR处理对茶树叶片光合特性、Rubisco与FBPase活性及其相关基因表达量的影响。结果表明外源EBR（0.1βmg·L^-1）处理后,龙井43、清明早和香菇寮白毫的叶片净光合速率分别提高了49.06%、45.49%和92.34%;最大羧化速率（Vc_max）分别提高了21.82%、21.68%和33.47%,最大再生速率（J_max）分别提高了17.16%、23.86%和23.23%;Rubisco和FBPase活性显著提高（P<0.05）;Rubisco和FBPase相关基因表达量显著（P<0.05）提高。可见,外源EBR可以通过调控基因表达提高茶树叶片中Rubisco和FBPase的活性以及Rubisco最大羧化速率和RuBP的最大再生速率,从而促进茶树光合碳固定,提高茶树的光合速率。

关键词: 茶树, 2-4表油菜素内酯, 光合特性, 基因表达

Abstract: 24-epibrassinolide (EBR) is an important plant hormone, which plays an important role in a variety of adverse resistance in plants. The effects of exogenous EBR on photosynthesis, Rubisco and FBPase activity and the expression of related-gene from tea leaves (Camellia sinensis cv. ‘Longjing 43’, ‘Xiangguliaobaihao’, and ‘Qingmingzao’) under field condition were investigated. It was showed that after exogenous EBR (0.1βmg·L^-1) treatment: Net photosynthetic rate of these three cultivars increased by 49.06%, 45.49% and 92.34% respectively. Vc_max increased by 21.82%, 21.68 % and 21.68%, and J_max increased by 17.16%, 23.86% and 23.86%, respectively. The activity of Rubisco and FBPase increased significantly. Furthermore, the expression of Rubisco and FBPase related-gene increased significantly. These results suggested that application of exogenous EBR could enhance Vc_max, J_max and the activity of Rubisco and FBPase effectively, which ultimately promoted the photosynthetic carbon fixation, and the photosynthetic rate of tea plants.

Key words: tea plant (Camellia sinensis), 24-epibrassinolide, photosynthetic characteristics, gene expression

中图分类号:

李治鑫, 李鑫, 范利超, 韩文炎. 外源油菜素内酯对茶树光合特性的影响[J]. 茶叶科学, 2015, 35(6): 543-550. doi: 10.13305/j.cnki.jts.2015.06.005.

LI Zhixin, LI Xin, FAN Lichao, HAN Wenyan. Effects of Exogenous 24-epibrassinolide on the Photosynthetic Characteristics of Tea Plants (Camellia sinensis)[J]. Journal of Tea Science, 2015, 35(6): 543-550. doi: 10.13305/j.cnki.jts.2015.06.005.

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