外源水杨酸甲酯对高温胁迫下茶树光合作用和抗氧化酶的影响

doi:10.13305/j.cnki.jts.2018.04.003

摘要/Abstract

摘要： 近年来茶园高温灾害频发,而关于提高茶树耐热性的研究相对较少。本文以龙井43为试验材料,利用不同浓度水杨酸甲酯（MeSA）喷施茶苗后,在高温环境下（43℃）处理12βh,随后测定茶树叶片的净光合速率（Pn）,Rubisco最大羧化速率（V_c,_max）、RuBP最大再生速率（J_max）,电解质渗透率（EL）,丙二醛（MDA）含量以及抗氧化酶活性等相关指标。结果发现,1βmmol·L^-1 MeSA能够有效缓解高温导致的茶树Pn降低,维持V_c,_max和J_max稳定;高温导致茶树叶片EL和MDA含量迅速上升,而适当浓度的MeSA可显著降低高温环境下EL和MDA含量。此外,结果表明1βmmol·L^-1 MeSA能够提高APX和CAT活性,进而减少H₂O₂积累,减轻茶树细胞膜过氧化作用。综上所述,外源施用MeSA能够在一定程度上维持高温条件下植物叶片细胞光合系统稳定,提高茶树叶片的抗氧化酶活性,缓解氧化胁迫,最终提高茶树的耐热性。

关键词: 水杨酸甲酯, 茶树, 高温胁迫, 光合作用, 抗氧化酶

Abstract: In recent years, heat stress was more frequently occurred in tea gardens. However, few studies were focused on the approaches towards the improvement of heat tolerance in tea plants. In this study, Longjing 43 was used as experimental material to investigate the effects of various concentrations of MeSA on the net photosynthetic rate (Pn), the maximum carboxylation rate of Rubisco (V_c_,max), the maximum RuBP regeneration rate (J_max), electrolyte leakage (EL), MDA content and antioxidant enzyme activities in tea leaves under heat stress. Results showed that 1βmmol·L^-1 MeSA could significantly increase Pn, V_c,_max and J_max in tea leaves under heat stress. EL and MDA content in tea leaves increased significantly after heat stress, while the application of MeSA attenuated heat-induced increases in EL and MDA. In addition, the application of 1βmmol·L^-1 MeSA stimulated the activities of APX and CAT, leading to an efficient scavenging of hydrogen peroxide (H₂O₂) in tea leaves. In summary, we revealed that the applications of MeSA could improve photosynthetic capacity, strengthen the antioxidant system, reduce reactive oxygen species accumulation and lipid peroxidation in tea leaves under heat stress, and thus improve the tolerance of tea plants under heat stress.

Key words: salicylic acid, tea plant, heat stress, photosynthesis, antioxidant enzyme

中图分类号:

魏吉鹏, 李鑫, 王朝阳, 李洋, 张兰, 沈晨, 颜鹏, 张丽平, 韩文炎. 外源水杨酸甲酯对高温胁迫下茶树光合作用和抗氧化酶的影响[J]. 茶叶科学, 2018, 38(4): 353-362. doi: 10.13305/j.cnki.jts.2018.04.003.

WEI Jipeng, LI Xin, WANG Zhaoyang, LI Yang, ZHANG Lan, SHEN Chen, YAN Peng, ZHANG Liping, HAN Wenyan. Effects of Exogenous Salicylic Acid on Photosynthesis and Antioxidant Enzymes of Tea Plants under High Temperature[J]. Journal of Tea Science, 2018, 38(4): 353-362. doi: 10.13305/j.cnki.jts.2018.04.003.

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