分散固相萃取-超高效液相色谱-串联质谱法测定不同茶类中2,4-表芸苔素内酯残留

doi:10.13305/j.cnki.jts.2018.06.005

Abstract

Abstract: An efficient method for analyzing 2,4-Epibrassinolide in different teas was developed based on ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Homogenized samples were extracted with acetonitrile solution, cleaned up with three sorbents including C₁₈, strong anion exchanger (SAX) and graphitized carbon black (GCB). The compound was separated on a HSS T3 column using electrospray Ionization (ESI) mode, detected by scheduled multiple reaction monitoring (SMRM) and quantified by matrix-matched external standard curves. The compound showed good linearity between the concentration of 0.8-800βμg·L^-1, with high significant correlation (R²>0.999). The limit of quantitation (LOQ, S/N=10) ranged from 0.55 to 1.46βμg·kg^-1. In addition, the spiked (20, 40, 200βμg·kg^-1) recoveries of tea samples were 75.5%-93.6%, and RSDs were 0.4%-7.0%. The method was accurately and sensitively applied to commercial tea samples, which fulfilled all requirement for the determination of 2,4-Epibrassinolide in tea samples.

Key words: tea, 2, 4-Epibrassinolide, brassinolide, brassinosteroids, ultra performance liquid chromatography- tandem mass spectrometry, dispersive solid phase extraction

CLC Number:

ZHU Li, CHEN Hongping, CHAI Yunfeng, MA Guicen, HAO Zhenxia, WANG Chen, LIU Xin, LU Chengyin. Determination of 2,4-Epibrassinolide in Different Teas by Ultra Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry with Dispersive Solid Phase Extraction[J]. Journal of Tea Science, 2018, 38(6): 589-594.

References 18

[1]	Grove M D, Spencer G F, Rohwedder W K, et al.A unique plant growth promoting steroid from Brassica napus pollen[J]. Nature, 1979, 281: 216-217.
[2]	王广聚, 董服灿, 董建军. 24-表油菜素内酯的合成及应用研究[J].Pesticides, 2001, 40(6) : 12-15.
[3]	朱永兴. 茶树生长调节剂的研究与应用[J]. 中国茶叶, 1998, 20(2) : 12-13.
[4]	陆剑飞. 四种植物生长调节剂对茶叶产量与品质的影响[J]. 浙江农业学报, 2006, 18(3) : 192-194.
[5]	Lee K J, Johnson V, Blakley B.The effect of exposure to a commercial 2,4-D formulation during gestation on the immune response in CD-1 mice[J]. Toxicology, 2001, 165(1) : 39-49.
[6]	Erin N, Afacan B, Ersoy Y.Gibberellic acid, a plant growth regulator, increases mast cell recruitment and alters Substance P levels[J]. Toxicology, 2008, 254(1/2) : 75-81.
[7]	Tuluce Y, Celik I.Effects of indole-3-acetic acid and kinetin on the adenosine deaminase activity in various tissues of rats[J]. Asian Journal of Chemistry, 2006, 18(1) : 528-532.
[8]	Cao L D, Yang J, Li X H, et al.Determination of brassinolide analogs by high-performance liquid chromatography with evaporative light scattering detection[J]. Analytical Letters, 2015, 48(4): 575-585.
[9]	Lv T, Zhao X E, Zhu S Y.et al.Development of an efficient hplc fluorescence detection method for brassinolide by ultrasonic-assisted dispersive liquid-liquid microextraction coupled with derivatization[J]. Chromatographia, 2014, 77(23/24): 1653-1660.
[10]	Oklestkova J, Tarkowska D, Eyer L, et al.Immunoaffinity chromatography combined with tandem mass spectrometry: A new tool for the selective capture and analysis of brassinosteroid plant hormones[J]. Talanta, 2017, 170: 432-440.
[11]	Ding J, Mao L J, Wang S T, et al.Determination of endogenous brassinosteroids in plant tissues using solid-phase extraction with double layered cartridge followed by high-performance liquid chromatography- tandem mass spectrometry[J]. Phytochemical Analysis, 2013, 24(4): 386-394.
[12]	Huo F F, Wang X, Han Y H, et al.A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry[J]. Talanta, 2012, 99: 420-425.
[13]	Jiao W T, Xiao Y, Qian X S, et al.Optimized combination of dilution and refined QuEChERS to overcome matrix effects of six types of tea for determination eight neonicotinoid insecticides by ultra performance liquid chromatography- electrospray tandem mass spectrometry[J]. Food Chemistry, 2016, 210: 26-34.
[14]	Rahman M M, Abd El-Aty A M, Choi J H, et al. Consequences of the matrix effect on recovery of dinotefuran and its metabolites in green tea during tandem mass spectrometry analysis[J]. Food Chemistry, 2015, 168: 445-453.
[15]	Zhang K, Wong J W, Yang P, et al.Protocol for an electrospray ionization tandem mass spectral product ion library: development and application for identification of 240 pesticides in foods[J]. Analytical Chemistry, 2012, 84(13): 5677-5684.
[16]	诸力, 王晨, 陈红平, 等. 超高效液相色谱-串联质谱法同时测定茶叶中11种植物生长调节剂及吡虫啉、啶虫脒的残留[J]. 分析化学, 2017, 45(4): 529-536.
[17]	虞冰, 刘莉, 汪志威, 等. QuEChERS-超高效液相色谱-串联质谱法快速测定草莓中2,4-表芸苔素内酯残留量[J]. 浙江农业科学, 2017, 58(9): 1538-1540, 1545.
[18]	Kittlaus S, Schimanke J, Kempe G, et al.Assessment of sample cleanup and matrix effects in the pesticide residue analysis of foods using postcolumn infusion in liquid chromatography-tandem mass spectrometry[J]. Journal of Chromatography A, 2011, 1218(46): 8399-8410.

Determination of 2,4-Epibrassinolide in Different Teas by Ultra Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry with Dispersive Solid Phase Extraction

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 18

Related Articles 15

Metrics

Comments

Recommended 10

[1]	GAO Jianjian, CHEN Dan, PENG Jiakun, WU Wenliang, CAI Liangsui, CAI Yawei, TIAN Jun, WAN Yunlong, SUN Weijiang, HUANG Yan, WANG Zhe, LIN Zhi, DAI Weidong. Comparison on Chemical Components of Yunnan and Fuding White Tea Based on Metabolomics Approach [J]. Journal of Tea Science, 2022, 42(5): 623-637.
[2]	CHEN Qiyu, MA Jianqiang, CHEN Jiedan, CHEN Liang. Genetic Diversity of Mature Leaves of Tea Germplasms Based on Image Features [J]. Journal of Tea Science, 2022, 42(5): 649-660.
[3]	LI Yanchun, WANG Hang, LI Zhaowei, YE Jing, WANG Yixiang. Ameliorative Effect of Several Measures on Soil Physicochemical Properties and Microbial Community Structures in Acidified Tea Gardens [J]. Journal of Tea Science, 2022, 42(5): 661-671.
[4]	SUN Yue, WU Jun, WEI Chaoling, LIU Mengyue, GAO Chenxi, ZHANG Lingzhi, CAO Shixian, YU Shuntian, JIN Shan, SUN Weijiang. Screening of Tea Germplasm Resistant to Matsumurasca onukii and Dendrothrips minowai Priesner and Analysis of Resistance-related Factors [J]. Journal of Tea Science, 2022, 42(5): 689-704.
[5]	CHEN Yuhong, GAO Ying, HAN Zhen, YIN Junfeng. Analysis of the Saponin Contents and Composition in Tea Seeds of Different Germplasms [J]. Journal of Tea Science, 2022, 42(5): 705-716.
[6]	CHEN Hui, YANG Liling, CHEN Jinhua, HUANG Jian'an, GONG Yushun, LI Shi. Effect of Temperature-controlled Pile-fermentation on Aroma Quality of Primary Dark Tea [J]. Journal of Tea Science, 2022, 42(5): 717-730.
[7]	CHEN Ke, WANG Yuanzhu, YANG Xiaoying, ZHANG Dongying, ZHU Qiangqiang. Preparation of Nanoparticules with Chitosan Complexed β-lactoglobulin Loaded EGCG and their Effects on Blood Glucose in Diabetic Mice [J]. Journal of Tea Science, 2022, 42(5): 731-739.
[8]	LI Zheng, LIU Ding, HUO Zenghui, CHEN Fuqiao. Analysis on the Competitiveness and Complementarity of Tea Trade between China and RCEP Members [J]. Journal of Tea Science, 2022, 42(5): 740-752.
[9]	WANG Yuyuan, LIU Renjian, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Expression Analysis and Functional Identification of CsTT2 R2R3-MYB Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2022, 42(4): 463-476.
[10]	LI Jing, LIN Cairong, HUANG Yan, DENG Xuming, WANG Yiqing, SUN Weijang. Effects of Tea Polyphenols on Agrobacterium-mediated Plant Genetic Transformation System [J]. Journal of Tea Science, 2022, 42(4): 477-490.
[11]	ZHAO Dongwei. Nomenclature, Typification, and Natural Distribution of Camellia sinensis var. assamica (Theaceae) [J]. Journal of Tea Science, 2022, 42(4): 491-499.
[12]	LIU Jianjun, ZHANG Jinyu, PENG Ye, LIU Xiaobo, YANG Yun, HUANG Tao, WEN Beibei, LI Meifeng. Effects of Light Waves on the Aroma Substances of Fresh Tea Leaves in Summer and Autumn During Spreading and the Quality of Final Green Tea [J]. Journal of Tea Science, 2022, 42(4): 500-514.
[13]	ZHANG Yinggen, XIANG Lihui, CHEN Lin, LIN Qingxia, SONG Zhenshuo, WANG Lili. Effects of Air Conditions Preset for Withering on Flavor Quality and Chemical Profiles of White Teas [J]. Journal of Tea Science, 2022, 42(4): 525-536.
[14]	LI Ziqiang, YANG Mei, ZHANG Xinzhong, LUO Fengjian, ZHOU Li, LOU Zhengyun, SUN Hezhi, WANG Xinru, CHEN Zongmao. Residue Determination of Sixteen Pesticides in Green Tea by UPLC-MS/MS [J]. Journal of Tea Science, 2022, 42(4): 537-548.
[15]	FANG Mengrui, LÜ Jun, RUAN Jianyun, BIAN Lei, WU Chuanyu, YAO Qing. Tea Buds Detection Model Using Improved YOLOv4-tiny [J]. Journal of Tea Science, 2022, 42(4): 549-560.