茶叶花青素合成、调控及功能的研究进展

doi:10.13305/j.cnki.jts.2018.06.007

Abstract

Abstract: Anthocyanins, a type of polyphenols and natural antioxidants, can benefit human health such as lowering blood lipids, pressure and against cancer. Purple tea cultivars enriched in anthocyanins, attract increasing attention in the market. Based on recent studies of anthocyanins, the types, biosynthesis pathway and physiological functions of anthocyanins in tea plants, as well as the internal and external factors affecting the biosynthesis of anthocyanins in tea plant were reviewed, which would provide theoretical foundation for the development and utilization of tea anthocyanins.

Key words: tea plant (Camellia sinensis), anthocyanins, biosynthesis, function

CLC Number:

S571.1

PANG Dandan, ZHANG Fen, ZHANG Yazhen, WEI Kang, WANG Liyuan, CHENG Hao. Research Advance on Biosynthesis, Regulation and Function of Anthocyanins in Tea Plant[J]. Journal of Tea Science, 2018, 38(6): 606-614.

References 76

[1]	Rashid K, Wachira F N, Nyabuga J N, et al.Kenyan purple tea anthocyanins ability to cross the blood brain barrier and reinforce brain antioxidant capacity in mice[J]. Nutritional Neuroscience, 2014, 17(4): 178-185.
[2]	Yang L, Ling W, Du Z, et al.Effects of Anthocyanins on cardiometabolic health: a systematic review and meta-analysis of randomized controlled trials[J]. Advances in Nutrition, 2017, 8(5): 684-693.
[3]	Hsu C P, Shih Y T, Lin B R, et al.Inhibitory effect and mechanisms of an anthocyanins-and anthocyanidins-rich extract from purple-shoot tea on colorectal carcinoma cell proliferation[J]. J Agric Food Chem, 2012, 60(14): 3686-3692.
[4]	David V, Ana R M, Giulia C, et al.Polyphenols and human health: prevention of disease and mechanisms of action[J]. Nutrients, 2010, 2(11): 1106-1131.
[5]	潘亚燕, 吴华玲, 李家贤, 等. 红紫芽茶利用研究进展[J]. 广东农业科学, 2015, 42(1): 8-12.
[6]	梅菊芬, 徐德良, 汤茶琴, 等. 茶树花青素及其种质资源的研究和利用进展[J]. 热带农业工程, 2013, 37(1): 42-46.
[7]	Martin C, Gerats T.Control of pigment biosynthesis genes during petal development[J]. Plant Cell, 1993, 5(10): 1253-1264.
[8]	Ogata J, Kanno Y, Itoh Y, et al.Plant biochemidtry: anthocyain biosynthesis in roses[J]. Nature, 2005, 435(7043): 757-758.
[9]	Jiang L, Shen X, Shoji T, et al.Characterization and activity of anthocyanins in Zijuan tea (Camellia sinensis var. kitamura)[J]. Journal of Agricultural and Food Chemistry, 2013, 61(13): 3306-3310.
[10]	Lai Y S, Li S, Tang Q, et al.The dark-purple tea cultivar 'Ziyan' accumulates a large amount of delphinidin-related anthocyanins[J]. J Agric Food Chem, 2016, 64(13): 2719-2726.
[11]	Wang L, Pan D, Liang M, et al. Regulation of anthocyanin biosynthesis in purple leaves of Zijuan tea (Camellia sinensis var. kitamura) [J]. International Journal of Molecular Sciences, 2017, 18(4): 833. https://doi.org/10.3390/ijms18040833.
[12]	谷记平, 赵淑娟. 功能型(特种茶)茶产品的开发和研究现状[J]. 中国茶叶, 2014, 36(11): 10-13.
[13]	王秋霜, 凌彩金, 刘淑媚, 等. 红紫芽茶叶花青素提取分离及pH稳定性初探[J]. 中国农学通报, 2014, 30(9): 291-296.
[14]	Jez J M, Bowman M E, Dixon R A, et al.Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase[J]. Nature Structural Biology, 2000, 7(9): 786-791.
[15]	Gensheimer M, Mushegian A.Chalcone isomerase family and fold: no longer unique to plants[J]. Protein Science, 2004, 13(2): 540-544.
[16]	Punyasiri P A, Abeysinghe I S, Kumar V, et al.Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathways[J]. Archives of Biochemistry and Biophysics, 2004, 431(1): 22-30.
[17]	Onyilagha J C, Grotewold E, Pandalai S G.The biology and structural distribution of surface flavonoids[J]. Recent Research Developments in Plant Science, 2004, 136(2): 53-71.
[18]	Harborne J B.The flavonoids: recent advances [M]//Goodwin T W. Plant Pigments. London: Academic Press, 1988: 299-343.
[19]	Nakayama T, Suzuki H, Nishino T.Anthocyanin acyltransferases: specificities, mechanism, phylogenetics, and applications[J]. Journal of Molecular Catalysis B Enzymatic, 2003, 23(2): 117-132.
[20]	Springob K, Nakajima J, Yamazaki M, et al.Recent advances in the biosynthesis and accumulation of anthocyanins[J]. Natural Product Reports, 2003, 20(3): 288-303.
[21]	Winkel-Shirley B.Evidence for enzyme complexes in the phenylpropanoid and flavonoid pathways[J]. Physiologia Plantarum, 1999, 107(1): 142-149.
[22]	Alfenito MR, Souer E, Goodman CD, et al.Functional complementation of anthocyanin sequestration in the vacuole by widely divergent glutathione S-transferases[J]. The Plant cell, 1998, 10(7): 1135-1149.
[23]	Larsen ES, Alfenito MR, Briggs WR, et al.A carnation anthocyanin mutant is complemented by the glutathione S-transferases encoded by maize Bz2 and petunia An9[J]. Plant Cell Reports, 2003, 21(9): 900-904.
[24]	张亚真, 张芬, 王丽鸳, 等. 植物谷胱甘肽转移酶在类黄酮累积中的作用[J]. 植物生理学报, 2015, 51(11): 1815-1820.
[25]	Zhao J, Dixon RA.The ‘ins’ and ‘outs’ of flavonoid transport[J]. Trends in Plant Science, 2009, 15(2): 72-80.
[26]	Kitamura S, Shikazono N, Tanaka A.TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis[J]. Plant Journal for Cell and Molecular Biology, 2004, 37(1): 104-114.
[27]	Loïc Lepiniec, Isabelle Debeaujon, JeanMarc Routaboul, et al. Genetics and biochemistry of seed flavonoids[J]. Annual review of plant biology, 2006, 57(1): 405-430.
[28]	Mueller LA, Goodman CD, Silady RA, et al.AN9, a petunia glutathione S-transferase required for anthocyanin sequestration, is a flavonoid-binding protein[J]. Plant physiology, 2000, 123(4): 1561-1570.
[29]	张亚真, 韦康, 王丽鸳,等. 基于转录组测序对茶树GST基因表达的研究[J]. 茶叶科学, 2016, 36(5): 513-522.
[30]	Wei K, Zhang Y, Wu L, et al.Gene expression analysis of bud and leaf color in tea[J]. Plant Physiology and Biochemistry, 2016, 107: 310-318.
[31]	Weiss D.Regulation of flower pigmentation and growth: multiple signaling pathways control anthocyanin synthesis in expanding petals[J]. Physiologia Plantarum, 2010, 110(2): 152-157.
[32]	Mano H, Ogasawara F, Sato K, et al.Isolation of a regulatory gene of anthocyanin biosynthesis in tuberous roots of purple-fleshed sweet potato[J]. Plant Physiology, 2007, 143(3): 1252-1268.
[33]	Gonzalez A, Zhao M, Leavitt J M, et al.Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings[J]. Plant Journal, 2008, 53(5): 814-827.
[34]	Koes R, Verweij W, Quattrocchio F.Flavonoids: a colorful model for the regulation and evolution of biochemical pathways[J]. Trends in Plant Science, 2005, 10(5): 236-242.
[35]	S A Goff, K C Cone, V L Chandler.Functional analysis of the transcriptional activator encoded by the maize B gene: evidence for a direct functional interaction between two classes of regulatory proteins[J]. Genes & development, 1992, 6(5): 864-875.
[36]	Hernandez J, Heine G, Irani NG, et al.Different mechanisms participate in the R-dependent activity of the R2R3 MYB transcription factor C1[J]. Journal of Biological Chemistry. 2004, 279: 48205-48213.
[37]	Jiang X, Huang K, Zheng G, et al.CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis[J]. Plant Science, 2018, 270: 209-220.
[38]	He X, Zhao X, Gao L, et al. Isolation and characterization of key genes that promote flavonoid accumulation in purple-leaf tea (Camellia sinensis L.) [J]. Scientific Reports, 2018, 8(1): 130. www.nature.com/articles/s41598-017-18133-z.
[39]	El-Sharkawy Islam, Liang Dong, Xu Kenong.Transcriptome analysis of an apple (Malus×domestica) yellow fruit somatic mutation identifies a gene network module highly associated with anthocyanin and epigenetic regulation[J]. Journal of experimental botany, 2015, 66(22): 7359-7376.
[40]	Singh R, Low E T, Ooi L C, et al.The oil palm VIRESCENS gene controls fruit colour and encodes a R2R3-MYB[J]. Nature Communications, 2014, 5: 4106. doi: 10.1038/ncomms5106.
[41]	Wang Z, Meng D, Wang A, et al.The methylation of the PcMYB10 promoter is associated with green-skinned sport in max red bartlett pear[J]. Plant Physiology, 2013, 162(2): 885-896.
[42]	Sun B, Zhu Z, Cao P, et al. Purple foliage coloration in tea (Camellia sinensis L.) arises from activation of the R2R3-MYB transcription factor CsAN1 [J]. Scientific Reports, 2016, 6: 32534. www.nature.com/articles/srep32534.
[43]	Li M, Li Y, Guo L, et al.Functional characterization of tea (Camellia sinensis) MYB4a transcription factor using an integrative approach[J]. Frontiers in Plant Science, 2017, 8: 943. doi: 10.3389/fpls.2017.00943.
[44]	E Grotewold.The genetics and biochemistry of floral pigments[J]. Annual review of plant biology, 2006, 57(1): 761-780.
[45]	马春雷, 姚明哲, 王新超, 等. 利用基因芯片筛选茶树芽叶紫化相关基因[J]. 茶叶科学, 2011, 31(1): 59-65.
[46]	赵磊. 茶树类黄酮合成转录因子筛选及ANR基因功能验证[D]. 合肥: 安徽农业大学, 2013.
[47]	Borevitz J O, Xia Y, Blount J, et al.Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis[J]. Plant Cell, 2000, 12(12): 2383-2393.
[48]	Jin-Seog Kim, Byung-Hoi Lee, So-Hee Kim, et al.Responses to environmental and chemical signals for anthocyanin biosynthesis in non-chlorophyllous corn ( Zea mays, L.) leaf[J]. Journal of Plant Biology, 2006, 49(1): 16-25.
[49]	Mol J, Jenkins G, SchãFer E, et al. Signal perception, transduction, and gene expression involved in anthocyanin biosynthesis[J]. Critical Reviews in Plant Sciences, 1996, 15(5/6): 525-557.
[50]	李智. 不同环境因子调控茶树紫色芽叶形成的分子机制研究[D]. 泰安: 山东农业大学, 2014.
[51]	孙彬妹. 茶树MYB转录因子CsAN1调控花青素的作用机制研究[D]. 广州: 华南农业大学, 2016.
[52]	Dong Y H, Beuning L, Davies K, et al.Expression of pigmentation genes and photo-regulation of anthocyanin biosynthesis in developing Royal Gala apple flowers[J]. Functional Plant Biology, 1998, 25(2): 245-252.
[53]	Chatterjee M, Sharma P, Khurana J P.Cryptochrome 1 from Brassica napus is up-regulated by blue light and controls hypocotyl/stem growth and anthocyanin accumulation[J]. Plant Physiology, 2006, 141(1): 61-74.
[54]	张泽岑, 王能彬. 光质对茶树花青素含量的影响[J]. 四川农业大学学报, 2002, 20(4): 337-339.
[55]	Christie P J, Alfenito M R, Walbot V.Impact of low-temperature stress on general phenylpropanoid and anthocyanin pathways: Enhancement of transcript abundance and anthocyanin pigmentation in maize seedlings[J]. Planta, 1994, 194(4): 541-549.
[56]	Antonio Leyva, Julio Salinas, José Miguel Martinez-Zapater. Low temperature induces the accumulation of Phenylalanine Ammonia-Lyase and Chalcone Synthase mRNAs of Arabidopsis thaliana in a light-dependent manner[J]. Plant physiology, 1995, 108(1): 39-46.
[57]	Shaked-Sachray L, Weiss D, Reuveni M, et al.Increased anthocyanin accumulation in aster flowers at elevated temperatures due to magnesium treatment[J]. Physiologia Plantarum, 2002, 114(4): 559-565.
[58]	Solfanelli C, Poggi A, Loreti E, et al.Sucrose-specific induction of the anthocyanin biosynthetic pathway in Arabidopsis[J]. Plant Physiology, 2006, 140(2): 637-646.
[59]	Weiss D, Halevy A H.Stamens and gibberellin in the regulation of corolla pigmentation and growth in Petunia hybrid[J]. Planta, 1989, 179(1): 89-96.
[60]	Feyissa D N, Løvdal T, Olsen K M, et al.The endogenous GL3, but not EGL3, gene is necessary for anthocyanin accumulation as induced by nitrogen depletion in Arabidopsis rosette stage leaves[J]. Planta, 2009, 230(4): 747-754.
[61]	Fritz C, Palacios-Rojas N, Feil R, et al.Regulation of secondary metabolism by the carbon-nitrogen status in tobacco: nitrate inhibits large sectors of phenylpropanoid metabolism[J]. Plant Journal for Cell & Molecular Biology, 2006, 46(4): 533-548.
[62]	Lovdal T, Olsen K M, Slimestad R, et al.Synergetic effects of nitrogen depletion, temperature, and light on the content of phenolic compounds and gene expression in leaves of tomato[J]. Phytochemistry, 2010, 71(5/6): 605-613.
[63]	Wolf-Rüdiger Scheible, Mark Stitt.Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen[J]. Plant Physiology, 2004, 136(1): 2483-2499.
[64]	Li-Li Zhou, Ming-Zhu Shi, De-Yu Xie.Regulation of anthocyanin biosynthesis by nitrogen in TTG1-GL3/TT8-PAP1-programmed red cells of Arabidopsis thaliana[J]. Planta, 2012, 236(3): 825-837.
[65]	Larbat R, Olsen K M, Slimestad R, et al.Influence of repeated short-term nitrogen limitations on leaf phenolics metabolism in tomato[J]. Phytochemistry, 2012, 77(1):119-128.
[66]	刘健伟. 基于组学技术研究氮素对于茶树碳氮代谢及主要品质成分生物合成的影响[D]. 北京: 中国农业科学院, 2016.
[67]	Neill S O, Gould K S.Anthocyanins in leaves: light attenuators or antioxidants?[J]. Functional Plant Biology, 2003, 30(8): 865-873.
[68]	Tattini M, Landi M, Brunetti C, et al.Epidermal coumaroyl anthocyanins protect sweet basil against excess light stress: multiple consequences of light attenuation[J]. Physiologia Plantarum, 2014, 152(3): 585-598.
[69]	Burger J, Edwards G E.Photosynthetic efficiency, and photodamage by UV and visible radiation, in red versus green leaf coleus varieties[J]. Plant & Cell Physiology, 1996, 37(3): 395-399.
[70]	张开明. 四季秋海棠(Begonia semperflorens)叶片中花色素苷的光保护作用和低温诱导机理[D]. 杭州: 浙江大学, 2010.
[71]	王良再, 胡彦波, 张会慧, 等. 植物叶片花青素的光破坏防御机制研究进展[J]. 应用生态学报, 2012, 23(3): 835-841.
[72]	Trevor A.Thorpe. Effect of mannitol and glucose-induced osmotic stress on growth, water relations, and solute composition of cell suspension cultures of poplar (Populus deltoides var. occidentals) in relation to anthocyanin accumulation[J]. In Vitro Cellular & Developmental Biology-Plant, 1994, 30(3): 164-170.
[73]	费旭元. 紫娟茶中花青素的提取分离及抗氧化活性研究[D]. 北京: 中国农业科学院, 2012.
[74]	林志城, 林伯儒, 徐治平, 等. 紫芽茶在抗肠癌保健活性与抑制机制[C]. 海峡两岸茶业学术研讨会. 2010.
[75]	Liang W, Lee A H, Binns C W, et al.Tea consumption and ischemic stroke risk: a case-control study in southern China[J]. Stroke, 2009, 40(7): 2480-2485.
[76]	梁名志, 夏涛. 特种紫茶降压活性物质初探[J]. 云南农业大学学报, 2003, 18(4): 378-381.

Research Advance on Biosynthesis, Regulation and Function of Anthocyanins in Tea Plant

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