茶树两个MYB转录因子基因的克隆及功能验证

doi:10.13305/j.cnki.jts.2014.01.005

Abstract

Abstract: R2R3-MYB in Sg4 subgroup probably participated in the regulation of lignin biosynthesis, which possibly play an important physiological role in plant growth and development. Two MYB transcription factors CsMYB4-5 and CsMYB4-6 in Sg4 subgroup were cloned by RACE technology. Investigation showed that the amino acid sequence of CsMYB4-5 showed 48.45% identity to AmMYB330 from Antirrhinum majus and 44.79% to AtMYB3 from Arabidopsis thaliana, while CsMYB4-6 showed 69.80% identity to AmMYB308 from Antirrhinum majus and 62.41% to AtMYB4 from Arabidopsis thaliana by bioinformatics analysis. The result of real-time fluorescent quantitative PCR showed that these two genes had all high expression levels in root while low expression levels in stem. The analysis of prokaryotic expression revealed that the recombinant CsMYB4-5 and CsMYB4-6 were expressed with a molecular weight of about 32 kD and 27 kD respectively. Compared with wild tobacco, transgenic tobacco with CsMYB4-6 gene appeared following symptoms: the veins of leaf tighten, lamina surface between veins with sags and crests, with white freckles in old leaf, but in transgenic tobacco with CsMYB4-5 gene the elder leaves turned yellow.

Key words: Camellia sinensis (L.), MYB transcription factor, expression analysis, prokaryotic expression analysis, transgenic tobacco analysis

CLC Number:

S571.1
Q52

GONG Niandi, GUO Lili, WANG Hongxue, ZHAO Lei, WANG Jie, WANG Wenzhao, LIU Yajun, WANG Yunsheng, GAO Liping, XIA Tao. Cloning and Functional Verification of Two MYB Transcription Factors in Tea Plant [Camellia sinensis (L.) ][J]. Journal of Tea Science, 2014, 34(1): 36-44.

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Cloning and Functional Verification of Two MYB Transcription Factors in Tea Plant [Camellia sinensis (L.) ]

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