茶树紫色芽叶花青苷组分分析及结构推测

doi:10.13305/j.cnki.jts.2014.03.010

Abstract

Abstract: A rapid method for analysis of anthocyanins in purple buds and leaves of tea plant was established by high-performance liquid chromatography (HPLC) based on the screening of mobile phase and the optimization of elution gradient. Using the established HPLC method, nine major chromatographic peaks were separated from crude extracts of anthocyanins from purple buds and leaves of tea plant. Further, the ultraviolet-visible spectrum and mass spectrum data of seven out the nine components were obtained by HPLC-DAD-MS/MS analysis. Combined with the literature data, five major components of anthocyanins were identified as delphindin-3-galactoside, cyanidin-3-gal-actoside, delphindin-3-rutinoside, cyanidin-3-rutinoside, and pelargonium-3-rutinoside. Additionally, each component was quantitatively analyzed using an external standard method, and crude extracts of anthocyanins were obtained at the purity of 32%. The proposed method is convenient and rapid with high accuracy and reproducibility, which can be applied for determining anthocyanins in purple buds and leaves of tea plant.

Key words: purple leaves of tea plant, anthocyanins, component, HPLC

CLC Number:

LI Zhi, WANG Riwei, ZHANG Lixia, HAN Xiaoyang. Analysis and Structural Identification of Anthocyanin Components in Purple Buds and Leaves of Tea Plant[J]. Journal of Tea Science, 2014, 34(3): 279-287.

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Analysis and Structural Identification of Anthocyanin Components in Purple Buds and Leaves of Tea Plant

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