茶树CsRAV2转录因子基因的克隆与表达特性分析

doi:10.13305/j.cnki.jts.2014.03.013

Abstract

Abstract: The RAV transcription factor, one subfamily of AP2/ERF family transcription factor, includes several genes that encode proteins involved in the development and regulation of abiotic/biotic resistance in higher plant. The CsRAV2 genes, which encoding to the RAV transcription factor, were cloned from tea plant (Camellia sinensis) cultivars ‘Anjibaicha’ and ‘Yingshuang’ by PCR and RT-PCR using DNA and cDNA as template, respectively. Then, nucleic acid and deduced amino acid sequence, phylogenetic tree, and molecular modeling were predicted and analyzed. The lengths of CsRAV2 genes from the two tea plant cultivars were 1 089 bp, encoding 362 amino acids. No intron was found in the CsRAV2 gene. The transcription factor of CsRAV2 contained two distinct DNA domains mainly found in higher plants RAV family factors, one AP2 domain together with one B3 domain. The CsRAV2 were hydrophilic protein. The protein of CsRAV2 from tea plant and AtRAV from Arabidopsis had similar three-dimension structure. Quantitative real-time PCR analysis of the expression profiles showed that the CsRAV2 gene was tissue-specific expressed in the two tea plant cultivars. The highest expression levels of the CsRAV2 gene were found in the root. The CsRAV2 gene was induced by high temperature, low temperature, PEG and high-salinity treatment, respectively. There were differences profiles between different teacultivars.

Key words: Camellia sinensis, transcription factor, RAV family factors, phylogenetic analysis, three-dimensional structure, expression analysis

CLC Number:

S571.1
S324

WU Zhijun, LI Xinghui, FANG Wanping, ZHOU Lin, ZHAO Zhen, ZHUANG Jing. Isolation of CsRAV2 Transcription Factor Gene of Tea Plant and its Expression Analysis[J]. Journal of Tea Science, 2014, 34(3): 297-306.

References 23

[1]	Riechmann J L, Meyerowitz E M.The AP2/EREBP Family of Plant Transcription Factors[J]. Biological Chemistry, 1998, 379(6): 633-646.
[2]	Kagaya Y, Ohmiya K, Hattori T.RAV1, a novel DNA-binding protein, binds to bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plants[J]. Nucleic Acids Research, 1999, 27(2): 470-478.
[3]	Sakuma Y, Liu Q, Dubouzet J G, et al. DNA-Binding Specificity of the ERF/AP2 Domain of Arabidopsis DREBs, Transcription Factors Involved in Dehydration- and Cold-Inducible Gene Expression[J]. Biochemical and Biophysical Research Communications, 2002, 290(3): 998-1009.
[4]	Zhuang J, Cai B, Peng R H, et al. Genome-wide analysis of the AP2/ERF gene family in Populus trichocarpa[J]. Biochemical and Biophysical Research Communications, 2008, 371(3): 468-474.
[5]	Nakano T, Suzuki K, Fujimura T, et al. Genome-Wide Analysis of the ERF Gene Family in Arabidopsis and Rice[J]. Plant Physiology, 2006, 140(2): 411-432.
[6]	Woo H R, Kim J H, Kim J, et al. The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis[J]. Journal of Experimental Botany, 2010, 61(14): 3947-3957.
[7]	HU Y X, WANG Y H, LIU X F, et al. Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development[J]. Cell Research, 2004, 14(1): 8-15.
[8]	刘翠芳. 水稻Hsp17.0、DREBA5-1和RAV1-1基因功能的初步研究[D]. 长沙: 湖南农业大学, 2011: 5-14.
[9]	Je B I, Piao H L, Park S J, et al. RAV-Like1 Maintains Brassinosteroid Homeostasis Via the Coordinated Activation of BRI1 and Biosynthetic Genes in Rice[J]. The Plant Cell, 2010, 22(6): 1777-1791.
[10]	Zhuang J, Sun C C, Zhou X R, et al. Isolation and characterization of an AP2/ERF-RAV transcription factor BnaRAV-1-HY15 in Brassica napus L. HuYou15[J]. Mol Biol Rep, 2011, 38(6): 3921-3928.
[11]	庄静, Jian Z, 熊爱生, 等. 甘蓝型油菜沪油15中BnaRAV- 2- HY15转录因子的克隆及表达分析[J]. 核农学报, 2010, 24(5): 941-947.
[12]	童启庆. 茶树栽培学[M]. 北京: 中国农业出版社, 2007: 4-33.
[13]	Tamura K, Peterson D, Peterson N, et al. MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods[J]. Molecular biology and evolution, 2011, 28(10): 2731-2739.
[14]	Schwede T, Kopp J, Guex N, et al. SWISS-MODEL: an automated protein homology-modeling server[J]. Nucleic Acids Research, 2003, 31(13): 3381-3385.
[15]	Pfaffl M W.A new mathematical model for relative quantification in real-time RT-PCR[J]. Nucleic Acids Research, 2001, 29(9): 2002-2007.
[16]	D. Allen M, Yamasaki K, Ohme-Takagi M, et al. A novel mode of DNA recognition by a β-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA[J]. The EMBO Journal, 1998, 17(18): 5484-5496.
[17]	Yamasaki K, Kigawa T, Inoue M, et al. Solution Structure of the B3 DNA Binding Domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1[J]. The Plant Cell, 2004, 16(12): 3448-3459.
[18]	Sohn K H, Lee S C, Jung H W, et al. Expression and functional roles of the pepper pathogen-induced transcription factor RAV1 in bacterial disease resistance, and drought and salt stress tolerance[J]. Plant Mol Biol, 2006, (61): 897-915.
[19]	Zhao L, Luo Q, Yang C, et al. A RAV-like transcription factor controls photosynthesis and senescence in soybean[J]. Planta, 2008, 227(6): 1389-1399.
[20]	Li C W, Su R C, Cheng C P, et al. Tomato RAV Transcription Factor Is a Pivotal Modulator Involved in the AP2/EREBP-Mediated Defense Pathway[J]. Plant Physiology, 2011, 156(1): 213-227.
[21]	陈林波, 房超, 王郁, 等. 茶树抗逆相关基因ERF的克隆与表达特性分析[J]. 茶叶科学, 2011, 31(1): 53-58.
[22]	常欣. 低温胁迫下茶树CsCBF1调控途径及其体外功能鉴定的初步研究[D]. 合肥: 安徽农业大学, 2012: 1-6.
[23]	陈林波, 李叶云, 王琴, 等. 茶树冷诱导基因RAV的克隆与表达特性分析[J]. 植物生理学通讯, 2010, 46(4): 354-358.

Isolation of CsRAV2 Transcription Factor Gene of Tea Plant and its Expression Analysis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 23

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City [J]. Journal of Tea Science, 2022, 42(5): 601-609.
[2]	ZHOU Hanchen, YANG Jihong, XU Yujie, WU Qiong, LEI Pandeng. Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis [J]. Journal of Tea Science, 2022, 42(5): 638-648.
[3]	WANG Yuyuan, LIU Renjian, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Expression Analysis and Functional Identification of CsTT2 R2R3-MYB Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2022, 42(4): 463-476.
[4]	XING Anqi, WU Zichen, XU Xiaohan, SUN Yi, WANG Genmei, WANG Yuhua. Research Advances of Fluoride Accumulation Mechanisms in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 301-315.
[5]	WANG Tao, WANG Yiqing, QI Siyu, ZHOU Zhe, CHEN Zhidan, SUN Weijiang. Identification and Transcriptional Regulation of CLH Gene Family and Expression Analysis in Albino Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 331-346.
[6]	LIU Fuhao, FAN Yangen, WANG Yu, MENG Fanyue, ZHANG Lixia. Screening and Identification of Chaperone CsHIPP26.1 Chelating Ions in Tea Cultivar ‘Huangjinya’ [J]. Journal of Tea Science, 2022, 42(2): 179-186.
[7]	YAN Yuting, LI Yujie, WANG Qian, TANG Meijun, GUO Huawei, LI Hongliang, SUN Liang. The Expression Profiles of Chemosensory Protein 8 Orthologs in Two Closely Related Tea Geometrid Species, Ectropis obliqua Prout and Ectropis grisescens Warren [J]. Journal of Tea Science, 2022, 42(2): 200-210.
[8]	LIU Yufei, PANG Dandan, LI Youyong, JIANG Huibing, TIAN Yiping, SUN Yunnan, CHEN Linbo. The Screening and Identification of Key Transcription Factor Genes for Theacrine Metabolism [J]. Journal of Tea Science, 2022, 42(1): 41-50.
[9]	WANG Pengjie, YANG Jiangfan, ZHANG Xingtan, YE Naixing. Research Advance of Tea Plant Genome and Sequencing Technologies [J]. Journal of Tea Science, 2021, 41(6): 743-752.
[10]	ZHOU Hanchen, LEI Pandeng. The Functional Identification of Two Alternative Splicing Transcripts of CsNES [J]. Journal of Tea Science, 2021, 41(6): 753-760.
[11]	YAN Minghui, LIU Ke, WANG Man, LYU Ying, ZHANG Qian. Complete Chloroplast Genome of Camellia sinensis cv. Xinyang 10 and Its Phylogenetic Evolution [J]. Journal of Tea Science, 2021, 41(6): 777-788.
[12]	LIU Miaomiao, ZANG Liansheng, SUN Xiaoling, ZHOU Zhongshi, YE Meng. Cloning and Expression Analysis of CsWRKY17 Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2021, 41(5): 631-642.
[13]	LIN Xinying, WANG Pengjie, CHEN Xuejin, GUO Yongchun, GU Mengya, ZHENG Yucheng, YE Naixing. Identification of LOX Gene Family in Camellia sinensis and Expression Analysis in the Process of White Tea Withering [J]. Journal of Tea Science, 2021, 41(4): 482-496.
[14]	WANG Yanding, WANG Huan, LI Nana, WANG Lu, HAO Xinyuan, WANG Yuchun, DING Changqing, YANG Yajun, WANG Xinchao, QIAN Wenjun. Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis [J]. Journal of Tea Science, 2021, 41(4): 497-510.
[15]	GU Mengya, WANG Pengjie, CHEN Xuejin, ZHENG Yucheng, GUO Yongchun, LIN Xinying, GAO Ting, HOU Binghao, YE Naixing. Identification of Alcohol Dehydrogenase Gene Family and Their Expression Analysis in the Withering Process of White Tea [J]. Journal of Tea Science, 2021, 41(3): 302-314.