茶叶提取物对B16小鼠黑色素瘤细胞的生物学效应及机理研究

doi:10.13305/j.cnki.jts.2014.05.007

Abstract

Abstract: The Bio-efficacy and mechanism of tea extract on mouse B16 melanoma cell in vitro were studied in this paper. Cells were treated with different concentration of TF1, EGCG and TF40, then the cell morphology was observed. The tyrosinase activity was determined by MTT method. The synthesis of melanin was determined using DOPA as substrate, and proliferation rate of B16 cells was determined by colorimetry, then analyzing the effects of drugs to these indexes. The results showed that the cell morphology was changed by the treatment of drugs. The tyrosinase activity, the synthesis of melanin and proliferation rate of B16 cells were all negatively related to the drugs concentration. Tea extracts and Vc can cause the inhibition of the synthesis of melanin, and resulted to white skin. The whiting effect of tea extracts is better than that of Vc.

Key words: tea extract, B16 melanoma cell, melanin, tyrosinase, mechanism

CLC Number:

Q946.84⁺1

CHEN Zhenchun, JIA Lingyan, MAO Zufa, KIM Eunhye, WANG Guangwei, TU Youying. Bio-efficacy of Tea Extract on Mouse B16 Melanoma Cell in Vitro and Its Mechanism[J]. Journal of Tea Science, 2014, 34(5): 465-472.

References

[1] 王自强. 皮肤黑素成因分析与美白祛斑功效评价体系的研究[J]. 化学工程与装备, 2008(2): 14-18.
[2] Yang CS.Cancer prevention by tea: animal studies, molecular mechanisms and human relevance[J]. Nature, 2009, 6: 429-439.
[3] Tu YY, Tang AB, Watanabe N.The Theaflavin Monomers Inhibit the Cancer Cells Growth in Vitro[J]. Acta Biochimica et Biophysica Sinica, 2004, 36(7): 508-512.
[4] Xu Y, Jin YX, Wu YY, et al. Isolation and purification of four individual theaflavins using semi-preparative high performance liquid chromatography[J]. Journal of Liquid Chromatography & Releted Technologies, 2010, 33(20): 1791-1801.
[5] Chang TS, Lin CH.Melanogenesis inhibitory activity of two generic drugs: Cinnarizine and trazodone in mouse B16 cells[J]. International Journal of Molecular Sciences, 2011, 12: 8787-8796.
[6] Mei X, Wu YY, Mao X, et al. Antagonism of phenanthrene cytotoxicity for human embryo lung fibroblast cell line HFL-I by green tea polyphenols[J]. Envitonmental Pollution, 2011, 159: 164-168.
[7] Maeda K, Fukuda M, Arbutin. Mechanism of its depigmenting action in melanocyte culture[J]. J Pharmacol Exp Ther, 1996, 276(2): 765.
[8] 康琰琰, 张美英, 刑少璟, 等. 几种天然活性物对黑色素细胞毒性及美白功效的比较[J]. 日用化学品科学, 2005, 35(6): 361-363.
[9] Yamaguchi Y, Brenner M, Hearing VJ.The regulation of skin pigmentation[J]. Boil Chem, 2007, 282(38): 27557-27561.
[10] 宋琦如, 金锡鹏. 黑素代谢的分子生物学机制与美白剂的研究评价[J]. 日用化学品科学, 2000, 23(5): 193.
[11] 贾爱群, 孙洋, 王建新. 美白剂的发展现状与其黑色素抑制机理的研究进展[J]. 日用化学工业, 2001, 31(2): 41-44.
[12] Lin J, Hsiumei C, Lin Y, ,et al. Natural products with skin—whitening effects [J]. Journal of food. Natural products with skin—whitening effects [J]. Journal of food and drug analysis, 2008, 16(2): l-10.
[13] Wang KH, Lin RD, Hsu FL, et al. Cosmetic application of selected traditional Chinese herbal medicines[J]. Journal of Ethnopharmacology, 2006, 106(3): 353-359.
[14] 屠幼英. 茶与健康[M]. 西安: 世界图书出版社, 2011: 190-192.
[15] 张玮, 高静, 张敏, 等. 7种美白化合物对皮肤黑素细胞的体外生物学效应[J]. 药学实践杂, 2011, 29(1): 24-26, 40.

Bio-efficacy of Tea Extract on Mouse B16 Melanoma Cell in Vitro and Its Mechanism

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	SUN Yue, WU Jun, WEI Chaoling, LIU Mengyue, GAO Chenxi, ZHANG Lingzhi, CAO Shixian, YU Shuntian, JIN Shan, SUN Weijiang. Screening of Tea Germplasm Resistant to Matsumurasca onukii and Dendrothrips minowai Priesner and Analysis of Resistance-related Factors [J]. Journal of Tea Science, 2022, 42(5): 689-704.
[2]	FANG Mengrui, LÜ Jun, RUAN Jianyun, BIAN Lei, WU Chuanyu, YAO Qing. Tea Buds Detection Model Using Improved YOLOv4-tiny [J]. Journal of Tea Science, 2022, 42(4): 549-560.
[3]	XING Anqi, WU Zichen, XU Xiaohan, SUN Yi, WANG Genmei, WANG Yuhua. Research Advances of Fluoride Accumulation Mechanisms in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 301-315.
[4]	ZHANG Zhipeng, CHENG Qinghua, XIE Jingkang, WAN Yuhe, TONG Huarong, CHEN Yingjuan. The Antifungal Effect and Mechanism of Berberine on Different Colletotrichum Species Causing Tea Brown Blight Disease [J]. Journal of Tea Science, 2022, 42(3): 367-375.
[5]	WANG Wei, CHEN Lin, WANG Weiwei, ZHANG Jianyong, JIANG Heyuan. Inhibition of Active Compounds in Tea on Melanin Formation [J]. Journal of Tea Science, 2021, 41(1): 7-18.
[6]	DONG Yanru, CAO Yanyan, LU Jiachun, MEI Sifan, HE Run, CHEN Ping. Crystallographic Study on the Complex of Catechins and Caffeine [J]. Journal of Tea Science, 2021, 41(1): 80-89.
[7]	OUYANG Jian, ZHOU Fang, LU Danmin, LI Xiuping, HUANG Jian'an, LIU Zhonghua. Research Progress of Tea Polysaccharides in Regulating Obesity [J]. Journal of Tea Science, 2020, 40(5): 565-575.
[8]	ZENG Jie, DENG Zhihui, FU Hongjuan, LIU Chang, GU Yi, ZOU Yixin, CHANG Hui. Theaflavin Activates Nrf2/HO-1 Pathway to Alleviate Oxidative Stress Injury in Vascular Endothelial Cells [J]. Journal of Tea Science, 2020, 40(5): 632-640.
[9]	LI Pan, ZHOU Hui, CAI Meisheng, KIM Eunhye, LIU Xiaobo, HE Puming, TU Youying. Improvement of White Tea on Cigarette Smoke-induced Chronic Obstructive Pulmonary Disease in Mice [J]. Journal of Tea Science, 2020, 40(5): 641-655.
[10]	GAO Chenxi, HUANG Yan, SUN Weijiang. Research Progress of Proanthocyanidins in Tea [J]. Journal of Tea Science, 2020, 40(4): 441-453.
[11]	PARK Soomi, KIM Eunhye, CHEN Xinghua, WANG Qianchao, HE Puming, TU Youying. The Inhibitory Role and Mechanism of White Tea Extracts on Pulmonary Fibrosis Induced by Nano-sized SiO₂ in Rats [J]. Journal of Tea Science, 2020, 40(2): 157-164.
[12]	XU Jiajia, GUANG Min, SHI Shulin, GAO Hongjian. Physiological and Molecular Mechanisms of Transmembrane Fluoride Uptake by Tea Roots [J]. Journal of Tea Science, 2019, 39(4): 365-371.
[13]	LIU Sai, LIU Shuoqian, LONG Jinhua, WU Dunchao, CHEN Yuhong, LIU Liping, LIU Zhonghua, TIAN Na. Functional Analysis of Glutathione Peroxidase Encoding Gene CsGPX1 in Camellia sinensis [J]. Journal of Tea Science, 2019, 39(4): 382-391.
[14]	LIU Zhonghua. The Development Process and Trend of Chinese Tea Comprehensive Processing Industry [J]. Journal of Tea Science, 2019, 39(2): 115-122.
[15]	ZENG Li, XIAO Wenjun. Advances in Brain Diseases Prevention and Treatment of L- theanine [J]. Journal of Tea Science, 2019, 39(2): 193-202.