黑茶对胎牛血清诱导脂肪变性的HepG2细胞的影响

doi:10.13305/j.cnki.jts.2013.01.004

Abstract

Abstract: Fu brick tea extract(FZ), Liubao tea extract(LB), Qing brick tea extract(QZ), Qianliang tea extract(QL) were assessed for the hepatic lipid-lowering potential when administered conditions contained 50% FBS in 48βh to establish the model of hepatic steatosis in vitro. MTT value, total triglyceride (TG), total cholesterol(TC) were used to evaluate the effect of dark tea on HepG2 steatosis. Compared with the model groups, cellular lipid accumulation was significantly suppressed in the treatment groups. Moreover,FZ, LB, QZ, QL all showed highly significant inhibitory effect against TG accumulation(P<0.01). FZ, QZ also significantly reduced the TC accumulation (P<0.01), however, the effect of LB and QL were obvious. These results showed dark tea extracts might reduce lipid accumulation in cell. It showed the suppressive effect on lipid accumulation.

Key words: dark tea, steatosis, total triglyceride, total cholesterol, HepG2 cells

CLC Number:

TS272.5⁺4

LI Su, ZHU Nan-nan, LIU Zhong-hua, JIANG Hua-jun. Effect of Dark Tea on Fetal Bovine Serum Fat Accumulation in HepG2 Cells[J]. Journal of Tea Science, 2013, 33(1): 45-52.

References 31

[1]	Jin HB, Gu ZY, Yu CH, et al. Association of nonalcoholic fatty liver disease with type 2 diabetes: clinical features and independent risk factors in diabetic fatty liver patients[J]. Hepatobiliary Pancreat Dis Int, 2005, 4(3): 389-392.
[2]	方继伟, 范建高. 非酒精性脂肪性肝病的治疗现状[J]. 中华肝脏病杂志, 2003, 11(2): 120-122.
[3]	Lee SM, Kim CW, Kim JK, et al. GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats[J]. Lipids, 2008, 43(5): 419-429.
[4]	Maron DJ, Lu GP, Cai NS, et al. Cholesterol-lowering effect of a theaflavin-enriched green tea extract: a randomized controlled trial[J]. Archives of internal medicine, 2003, 163(12): 1448.
[5]	Martins F, Noso TM, Porto VB, et al. Maté tea inhibits in vitro pancreatic lipase activity and has hypolipidemic effect on high-fat diet-induced obese mice[J]. Obesity, 2009, 18(1): 42-47.
[6]	Tanida M, Tsuruoka N, Shen J, et al. Effects of oolong tea on renal sympathetic nerve activity and spontaneous hypertension in rats[J]. Metabolism, 2008, 57(4): 526-534.
[7]	Yang YC, Lu FH, Wu JS, et al. The protective effect of habitual tea consumption on hypertension[J]. Archives of internal medicine, 2004, 164(14): 1534.
[8]	Yung L, Leung F, Wong W, et al. Tea polyphenols benefit vascular function[J]. Inflammopharmacology, 2008, 16(5): 230-234.
[9]	施兆鹏. 茶叶加工学[M]. 北京:中国农业出版社, 1997.
[10]	佐野満昭, 竹中康晴, 小島令子, 等. Effects of pu-erh tea on lipid metabolism in rats[J]. Chemical & pharmaceutical bulletin, 1986, 34(1): 221-228.
[11]	肖文军, 任国谱, 傅冬和, 等. 茯茶辅助调节血脂作用研究[J]. 茶叶科学, 2007, 27(3): 211-214.
[12]	Donati G, Stagni B, Piscaglia F, et al. Increased prevalence of fatty liver in arterial hypertensive patients with normal liver enzymes: role of insulin resistance[J]. Gut, 2004, 53(7): 1020-1023.
[13]	欧健. 大黄素对脂肪变性LO2肝细胞UCP2 mRNA表达的影响[D]. 广州: 暨南大学, 2009.
[14]	刘爱玲, 施兆鹏. 四川黑茶品质化学成分的研究[J]. 茶叶科学, 2004, 24(4): 266-269.
[15]	樊希承, 黄颖, 黄亦琦, 等. 脂肪肝肝脂与血脂的相关性研究[J]. 实用医技杂志, 2006, 13(20): 3531-3533.
[16]	傅冬和, 刘仲华, 黄建安, 等. 高通量筛选研究茯砖茶降脂功效[J]. 茶叶科学, 2006, 26(3): 209-214.
[17]	邓放明, 龚淑俐, 赵荣, 等. 冠突散囊菌胞外多糖的分离纯化[J]. 湖南农业大学学报: 自然科学版, 2008, 33(6): 662-666.
[18]	袁勇, 黄建安, 徐小江, 等. 茯茶中“金花”孢子粉提取物对体外诱导的非酒精性脂肪肝细胞内甘油三酯代谢的影响[J]. 茶叶科学, 2011, 31(2): 129-135.
[19]	Yen GC, Duh PD, Tsai HL.Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid[J]. Food Chemistry, 2002, 79(3): 307-313.
[20]	Sakagami H, Satoh K.Prooxidant action of two antioxidants: ascorbic acid and gallic acid[J]. Anticancer research, 1997, 17(1): 221-224.
[21]	吕海鹏, 林智, 谷记平, 等. 普洱茶中的没食子酸研究[J]. 茶叶科学, 2007, 27(2): 104-110.
[22]	折改梅, 张香兰, 陈可可, 等. 茶氨酸和没食子酸在普洱茶中的含量变化[J]. 云南植物研究, 2005, 27(5): 572-576.
[23]	胡绍德, 陈畅畅, 李大祥, 等. 黑茶中多酚组分和多酚总量分析[J]. 蚕桑茶叶通讯, 2011(2): 26-28.
[24]	刘仲华, 王增盛, 黄建安, 等. 黑茶初制中主要色素物质的变化与色泽品质的形成[J]. 茶叶科学, 1991, 11(3): 34-41.
[25]	齐桂年, 田鸿, 刘爱玲, 等. 四川黑茶品质化学成分的研究[J]. 茶叶科学, 2004, 24(4): 266-269.
[26]	Yeh C, Chen W, Chiang C, et al. Suppression of fatty acid synthase in MCF-7 breast cancer cells by tea and tea polyphenols: a possible mechanism for their hypolipidemic effects[J]. The pharmacogenomics journal, 2003, 3(5): 267-276.
[27]	Lin JK, Lin‐Shiau SY. Mechanisms of hypolipidemic and anti‐obesity effects of tea and tea polyphenols[J]. Molecular nutrition & food research, 2006, 50(2): 211-217.
[28]	Okamoto Y, Tanaka S, Haga Y.Enhanced GLUT2 gene expression in an oleic acid-induced in vitro fatty liver model[J]. Hepatology research, 2002, 23(2): 138-144.
[29]	Hwang LS, Lin LC, Chen NT, et al. Hypolipidemic effect and antiatherogenic potential of Pu-Erh tea[J]. ACS Publications, 2003, 859(5): 87-103.
[30]	吕海鹏, 谷记平, 林智, 等. 普洱茶的化学成分及生物活性研究进展[J]. 茶叶科学, 2007, 27(1): 8-18.
[31]	Imajo K, Fujita K, Yoneda M, et al. Hyperresponsivity to Low-Dose Endotoxin during Progression to Nonalcoholic Steatohepatitis Is Regulated by Leptin-Mediated Signaling[J]. Cell Metabolism, 2012, 16(1): 44-54.

Effect of Dark Tea on Fetal Bovine Serum Fat Accumulation in HepG2 Cells

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