“紫娟”普洱茶茶褐素的膜分离及其理化性质的初步研究

doi:10.13305/j.cnki.jts.2012.03.003

Abstract

Abstract: 3500Da decomposed dialysate and 100000Da undecomposed dialysate of the theabrownin in ‘zijuan’ pu-erh tea as the main content were obtained by using membrane technology and their physicochemical properties were studied. The result showed that the total acidic functional group, carboxyl group, phenolic hydroxyl group,tea polyphenols, polysaccharose content of 100000Da undecomposed dialysate were higher than those of 3500Da decomposed dialysate. The polysaccharide isolated from 100000Da undecomposed dialysate was composed of ribose, rhamnose, mannose, glucose and galactose,but these monose were not detected in 3500Da decomposed dialysate. It suggested that the 3500Da decomposed dialysate and 100000Da undecomposed dialysate contained the -OH and -COOH groups. It was suggested that the theabrownin of the ‘zijuan’ pu-erh tea belonged to benzene polyphenolic substance and contained of polyphenolic pigments, lipids, polysaccharide, protein and alkaloids.

Key words: ‘zijuan’, pu-erh tea, theabrownin, physicochemical properties, physicochemical properties, CP-GC-MS

CLC Number:

TS272.5⁺4

ZHANG Qin, DONG Li-xing, LI Gai-qing, GONG Jia-shun. Study on Membrane Separation and Its Physicochemical Properties of Theabrownin in ‘Zijuan’ Pu-erh Tea[J]. Journal of Tea Science, 2012, 32(3): 189-196.

References

[1] 周红杰, 龚加顺, 王星银, 等. 云南普洱茶: 第一版[M]. 昆明: 云南科技出版社, 2004.
[2] Sano M, Takenaka Y, Kojima R, et al. Effects of pu-erh tea on lipid metabolism in rats[J]. Chem Pharm Bull, 1986, 34(1): 221-228.
[3] Duh PD, Yen GC, Yen WJ, et al. Effects of pu-erh tea on oxidative damage and nitric oxide scavenging[J]. Journal of Agricultural and Food Chemistry, 2004, 52: 8169-8176.
[4] Wang BS, Yu HM, Chang LW, et al. Protective effects of pu-erh tea on LDL oxidation and nitric oxide generation in macrophage cells[J]. LWT-Food Science and Technology, 2008, 41, 1122-113.
[5] Kuo KL, Weng MS, Chiang CT, et al. Comparative studies on the hypolipidemic and growth suppressive effects of oolong, black, pu-erh, and green tea leaves in rats[J]. J Agric Food Chem, 2005, 53(2): 480-489.
[6] Cao ZH, Gu DH, Lin QY, et al. Effect of pu-erh tea on body fat and lipid profiles in rats with diet-induced obesity[J]. Phytotherapy Research, 2011, 25(2): 234-238.
[7] Chiang Chun-Te, Weng Meng-Shih, Lin-Shiau, et al. Pu-erh tea supplementation suppresses fatty acid synthase expression in the rat liver through downregulating akt and jnk signalings as demonstrated in human hepatoma HepG2 cells[J]. Oncol Res, 2006, 16(3): 119-128.
[8] Lu CH, Hwang SL.Polyphenol contents of Pu-Erh teas and their abilities to inhibit cholesterol biosynthesis in Hep G2 cell line[J]. Food Chem, 2008, 111: 67-71.
[9] Hou Y, Shao WF, Xiao R, et al. Pu-erh tea aqueous extracts lower atherosclerotic risk factors in a rat Hyperlipidemia model[J]. Experimental Gerontology, 2009, 44: 434-439.
[10] Ohe T, Marutani K, Nakase S.Catechins are not major components responsible for anti-genotoxic effects of tea extracts against nitroarenes[J]. Mutation Research, 2001, 496: 75-81.
[11] 包云秀, 夏丽飞, 李友勇, 等. 茶树新品种‘紫娟’[J]. 园艺学报, 2008, 35(6): 93.
[12] 杨大鹏. 云南普洱茶茶褐素主要化学成分的分离及结构鉴定[D]. 昆明: 云南农业大学, 2009.
[13] 秦谊, 龚加顺, 张惠芬, 等. 普洱茶茶褐素提取工艺及理化性质的初步研究[J]. 林产化学与工业, 2009, 29(6): 95-98.
[14] 黄意欢. 茶学实验技术[M]. 北京: 中国农业出版社, 1997: 125-127.
[15] HOSOYA T, KAWAMOTO H, SAKA S.Oxime-trimethylsilyl method for analysis of wood pyrolysat[J]. J Appl Anal Pyrolysis, 2006, 77: 121-126.
[16] POUWELS A D, EIJKEL G B, BOON J J.Curie-point pyrolysis-capillary gas chromatography-high-resolution mass spectrometry of microcrystalline cellulose[J]. J Anal Appl Pyrolysis, 1989, 14: 237-280.
[17] RAMIAH M V.Thermogravimetric and differential thermal analysis of cellulose,hemicellulose,and lignin[J]. Journal of Polymer Science, 1970, 14: 1323-1337.
[18] ELDER T J, SOLTES E J.Pyrolysis of lignocellulosic material phenolic constituents of a wood pyrolytic oil[J]. Wood and Fibre Science, 1980, 12(4): 217-226.
[19] FAIX O, MEIER D, FORTMANN I.Thermal degradation products of wood:Gas chromatographic separation and mass spectrometric characterisation of monomeric lignin derived products[J]. Holz als Roh-und Werkstoff, 1990, 48: 281-285.
[20] FAIX O, MEIER D, FORTMANN I.Thermal degradation products of wood:A collection of electron-impact(EI)mass spectra of monomeric lignin derived products[J]. Holz als Roh-und Werkstoff, 1990, 48: 351-354.

Study on Membrane Separation and Its Physicochemical Properties of Theabrownin in ‘Zijuan’ Pu-erh Tea

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	GONG Shouji, TENG Cuiqin, LIANG Dongyi, CAO Huiyi, MENG Yanfei, ZHANG Junwei, XIE Jiashi. In Vitro Study on Hypolipidemic Effects of Theabrownins in Liupao Tea [J]. Journal of Tea Science, 2020, 40(4): 536-543.
[2]	ZHENG Chengqin, MA Cunqiang, ZHANG Zhengyan, LI Xiaohong, WU Tingting, ZHOU Binxing. A Preliminary Study on the Degradation Pathway of Caffeine in Tea Microbial Solid-state Fermentation [J]. Journal of Tea Science, 2020, 40(3): 386-396.
[3]	MA Yan, CHEN Lijiao, LYU Caiyou, ZHAO Ming. Review on the Studies about Safety Assessment of Pu-erh Tea [J]. Journal of Tea Science, 2018, 38(3): 221-226.
[4]	LI Yali, XING Qianqian, TU Qing, ZHOU Hongjie. Study on the Safety of Pu-erh Tea Contaminated by Exogenous Aspergillus flavus [J]. Journal of Tea Science, 2017, 37(5): 513-522.
[5]	MA Cunqiang, ZHOU Binxing, WANG Hongzhen, WANG Pan. Screen and Identification of Fungi Strain Degrading Caffeine in Pu-erh Tea during Solid-state Fermentation [J]. Journal of Tea Science, 2017, 37(2): 211-219.
[6]	LIU Jian, PENG Chunxiu, SHENG Jun, GAO Bin, GONG Jiashun. Metabolomic Analysis of Rat Urine after Intragastric Infusion of Pu-erh Theabrownins [J]. Journal of Tea Science, 2016, 36(5): 491-499.
[7]	ZHU Shengyi, TONG Yuling, ZHAO Yi, LÜ Haipeng, LIN Zhi, SHEN Guoli, SUN Ting, SONG Zhenya. Effect of Pu-erh Tea on Long Chain Fatty Acid Metabolism and Expression of Tight Junction Proteins in the Rat Model of Non-alcoholic Fatty Liver Disease [J]. Journal of Tea Science, 2016, 36(3): 237-244.
[8]	YUE Suijuan, LIU Jian, GONG Jiashun. Effects of Theabrownin Extracted from Pu-erh Tea on the Intestinal Flora [J]. Journal of Tea Science, 2016, 36(3): 261-267.
[9]	HE Guowen, ZHONG Tongsheng, PENG Xiaoyun, LONG Liping, ZHAO Yunlin, ZHANG Weimin. Comparative Study on Component Characteristics and Inhibitory Activities on α-glucosidase of Theabrownin from Fuzhuan Brick Tea [J]. Journal of Tea Science, 2016, 36(1): 102-110.
[10]	ZHAO Zhenjun, LIU Qinjin. Advances on the Research of Fungi in Pu-erh Tea [J]. Journal of Tea Science, 2014, 34(3): 205-212.
[11]	REN Hongtao, ZHOU Bin, QIN Taifeng, XIA Kaiguo, ZHOU Hongjie. Study on Antioxidant Activity of Volatile Components from Pu-erh Tea [J]. Journal of Tea Science, 2014, 34(3): 213-220.
[12]	CHEN Meichun, CHEN Zheng, SHI Huai, LIU Bo, PAN Zhizhen, ZHU Yujing. Analysis on Characteristic Flavor Components of Aged Pu-erh Tea [J]. Journal of Tea Science, 2014, 34(1): 45-54.
[13]	LV Haipeng, ZHONG Qiusheng, SHI Jiang, ZHANG Yue, LIN Zhi. Study on Fingerprint on Volatile Constituents of Pu-erh Tea [J]. Journal of Tea Science, 2014, 34(1): 71-78.
[14]	LV Hai-peng, LIN Zhi, ZHANG Yue, LIANG Yue-rong. Study on the Content of the Major Mineral Elements in Pu-erh Tea [J]. Journal of Tea Science, 2013, 33(5): 411-419.
[15]	LI Ya-li, BI Ming, WEI Zhen-zhen, ZHOU Hong-jie. Application of Candida parapsilosis GPT-5-11 for Pu-erh Tea Fermentation [J]. Journal of Tea Science, 2013, 33(5): 436-442.