低咖啡碱茶树遗传群体的咖啡碱含量与分子变异分析

doi:10.13305/j.cnki.jts.2012.03.013

Abstract

Abstract: The caffeine content of 60 individuals of a low-caffeine population of tea plant was analyzed using HPLC and the molecular variance was studied using 42 EST-SSR markers, respectively. The results showed that the caffeine content of fresh weight ranged from 0.38% to 1.08%. Five individuals had lower caffeine content than female parent, and they can be used as breeding materials for further screening low-caffeine tea cultivars. One hundred and twenty-nine alleles were detected, each pair of primers could detect 2 to 7 alleles, an average of 3.86. The average number of Shannon-Weaver index (I) was 0.65. The polymorphism information content (PIC) of EST-SSR markers varied from 0.03 to 0.68, with average of 0.33. Three SSR markers, TM089, TM200 and TM211, related to variation of caffeine content were preliminary identified. It would be of important significance for screening excellent genetic resources and breeding new low-caffeine tea cultivars.

Key words: tea plant, caffeine content, molecular variance, SSR

CLC Number:

S571.1
Q347

WANG Xue-min, YAO Ming-zhe, JIN Ji-qiang, MA Chun-lei, CHEN Liang. Analysis of Caffeine Content and Molecular Variance of Low-caffeine Tea Plants[J]. Journal of Tea Science, 2012, 32(3): 276-282.

References

[1] 宛晓春. 茶叶生物化学: 第三版[M]. 北京: 中国农业出版社, 2003: 336.
[2] Bode AM, Dong ZG.The enigmatic effects of caffeine in cell cycle and cancer[J]. Cancer Letters, 2007, 247(1): 26-39.
[3] 吴命燕, 范方媛, 梁月荣, 等. 咖啡碱的生理功能及其作用机制[J]. 茶叶科学, 2010, 30(4): 235-242.
[4] Grosso LM, Bracken MB.Caffeine metabolism, genetics, and perinatal outcomes: A review of exposure assessment considerations during pregnancy[J]. Annals of Epidemiology, 2005, 15(6): 460-466.
[5] Tsuang YH, Sun JS, Chen LT, et al. Direct effects of caffeine on osteoblastic cells metabolism: the possible causal effect of caffeine on the formation of osteoporosis[J]. Journal of Orthopaedic Surgery and Research, 2006, 1(7): 1-7.
[6] 许勇泉, 尹军峰, 袁海波, 等. 茶叶脱咖啡因技术研究进展[J]. 茶叶科学, 2008, 28(1): 1-8.
[7] Gupta PK, Balyan HS, Sharma PC, et al. Microsatellites in plants: A new class of molecular markers[J]. Current Science, 1996, 70: 45-54.
[8] Hamwieh A, Udupa SM, Choumane W, et al. A genetic linkage map of Lens sp. based on microsatellite and AFLP markers and the localization of fusarium vascular wilt resistance[J]. Theoretical and Applied Genetics, 2005, 110: 669-677.
[9] Ausubel FM, Kingston RE, Seidman JG, et al.精编分子生物学实验指南: 第四版[M]. 马学军, 舒跃龙, 译. 北京: 科学出版社, 2004, 54-55.
[10] 乔婷婷, 马春雷, 周炎花, 等. 浙江省茶树地方品种与选育品种遗传多样性和群体结构的EST-SSR分析[J]. 作物学报, 2010, 36(5): 744-753.
[11] Ma JQ, Zhou YH, Ma CL, et al. Identification and characterization of 74 novel polymorphic EST-SSR markers in the tea plant, Camellia sinensis (Theaceae)[J]. American Journal of Botany, 2010, 97, e153-e156.
[12] Yao MZ, Ma CL, Qiao TT, et al. Diversity distribution and population structure of tea germplasms in China revealed by EST-SSR markers[J]. Tree Genetics & Genomes, 2012, 8: 205-220.
[13] 刘振, 王新超, 赵丽萍, 等. 基于EST-SSR的西南茶区茶树资源遗传多样性和亲缘关系分析[J]. 分子植物育种, 2008, 6(1): 100-110.
[14] Charters YM, Wilkinson MJ.The use of self-pollinated progenies as ‘in-groups’ for the genetic characterization of cocoa germplasm[J]. Theoretical and Applied Genetics, 2000, 100: 160-166.
[15] Liu K, Muse SV.PowerMarker: an integrated analysis environment for genetic marker data[J]. Bioinformatics, 2005, 21(9): 2128-2129.
[16] Nei M.Analysis of gene diversity in subdivided population[J]. Proceedings of the National Academy of Sciences of the United States of America, 1973, 70(12): 3321-3323.
[17] Botstein D, White RL, Skolnick M, et al. Construction of a genetic linkage maps in man using restriction fragment length polymorphisms[J]. The American Journal of Human Genetics, 1980, 32(3): 314-331.
[18] Kato M, Mizuno K, Fujimura T, et al. Purification and characterization of caffeine synthase from tea leaves[J]. Plant Physiology, 1999, 120: 579-586.
[19] Kato M, Mizuno K, Crozier A, et al. Caffeine synthase gene from tea leaves[J]. Nature, 2000, 406: 956-957.
[20] 冯艳飞, 梁月荣. 茶树S-腺苷甲硫氨酸合成酶基因的克隆和序列分析[J]. 茶叶科学, 2001, 21(1): 21-25.
[21] Keya CA, Crozier A, Ashihara H.Inhibition of caffeine biosynthesis in tea (Camellia sinensis) and coffee (Coffea arabica) plants by ribavirin[J]. FEBS Letters, 2003, 554(3): 473-477.
[22] Mohanpuria P, Kumar V, Ahuja PS, et al. Producing low-caffeine tea through post-transcriptional silencing of caffeine synthase mRNA[J]. Plant Molecular Biology, 2011, 76(6): 523-534.
[23] Mohanpuria P, Kumar V, Ahuja PS, et al. Agrobacterium-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis L[J]. Molecular Biotechnology, 2011, 48(3): 235-243.

Analysis of Caffeine Content and Molecular Variance of Low-caffeine Tea Plants

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City [J]. Journal of Tea Science, 2022, 42(5): 601-609.
[2]	CHEN Qiyu, MA Jianqiang, CHEN Jiedan, CHEN Liang. Genetic Diversity of Mature Leaves of Tea Germplasms Based on Image Features [J]. Journal of Tea Science, 2022, 42(5): 649-660.
[3]	SUN Yue, WU Jun, WEI Chaoling, LIU Mengyue, GAO Chenxi, ZHANG Lingzhi, CAO Shixian, YU Shuntian, JIN Shan, SUN Weijiang. Screening of Tea Germplasm Resistant to Matsumurasca onukii and Dendrothrips minowai Priesner and Analysis of Resistance-related Factors [J]. Journal of Tea Science, 2022, 42(5): 689-704.
[4]	WANG Yuyuan, LIU Renjian, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Expression Analysis and Functional Identification of CsTT2 R2R3-MYB Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2022, 42(4): 463-476.
[5]	LIU Renjian, WANG Yuyuan, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants [J]. Journal of Tea Science, 2022, 42(3): 347-357.
[6]	YANG Ni, LI Yimin, Li Jingwen, TENG Ruimin, CHEN Yi, WANG Yahui, ZHUANG Jing. Effects of Exogenous 5-ALA on the Chlorophyll Synthesis and Fluorescence Characteristics and Gene Expression of Key Enzymes in Tea Plants under Drought Stress [J]. Journal of Tea Science, 2022, 42(2): 187-199.
[7]	CHEN Xiaomin, ZHAO Feng, WANG Shuyan, SHAO Shuxian, WU Wenxi, LIN Qin, WANG Pengjie, YE Naixing. Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea [J]. Journal of Tea Science, 2022, 42(1): 18-28.
[8]	TANG Lei, XIAO Luodan, HUANG Yifan, XIAO Bin, GONG Chunmei. Cloning and Magnesium Transport Function Analysis of CsMGTs Genes in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2021, 41(6): 761-776.
[9]	LI Changle, GE Yue, YAN Meilin, LI Hui, LIN Qingqing, WANG Pu, ZHAO Hua, WANG Mingle, WANG Yu, GUO Fei, NI Dejiang. Analysis of Genetic Diversity and Population Structure of 32 Tea Landraces in China [J]. Journal of Tea Science, 2021, 41(5): 619-630.
[10]	MAO Juan, JIANG Hongjian, YANG Rubing, LI Chongxing, MA Chengying, CHEN Liang, MA Jianqiang. Genetic Diversity and Population Structure of Wild and Cultivated Camellia taliensis Populations [J]. Journal of Tea Science, 2021, 41(4): 454-462.
[11]	WU Wanfu, LYU Shidong, YANG Xuefang, LI Yue, ZHANG Jiguang. Study of Characteristic Indexes and Fatty Acid Composition in Big-leaf Tea Plant (Camellia sinensis var. assamica) Seed Oils from Yunnan Province [J]. Journal of Tea Science, 2021, 41(4): 463-470.
[12]	LIU Dingding, WANG Junya, TANG Rongjin, CHEN Liang, MA Chunlei. Genome-wide Identification of PPR Gene Family and Expression Analysis of Albino Related Genes in Tea Plants [J]. Journal of Tea Science, 2021, 41(2): 159-172.
[13]	ZHANG Qunfeng, NI Kang, YI Xiaoyun, LIU Meiya, RUAN Jianyun. Advances of Magnesium Nutrition in Tea Plant [J]. Journal of Tea Science, 2021, 41(1): 19-27.
[14]	CHEN Yao, ZHANG Weifu, REN Hengze, XIONG Fei, ZHANG Haojie, YAO Lina, LIU ying, WANG Lu, WANG Xinchao, YANG Yajun, HAO Xinyuan. Genome-wide Investigation and Expression Analysis of DNA Demethylase Genes in Tea Plant (Camellia Sinensis) [J]. Journal of Tea Science, 2021, 41(1): 28-39.
[15]	JIN Ke, HUANG Jian'an, XIONG Ligui, LIU Shuoqian, QIN Xiaohong, PENG Jing, LI Yinhua, LI Juan. Research of Theanine-related Genes Expressed in Etiolated Tea Plant (Camellia Sinensis) [J]. Journal of Tea Science, 2021, 41(1): 40-47.